Labshake search
Citations for Qiagen :
251 - 300 of 926 citations for Recombinant Influenza A virus HA protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: DNase/RNase treated VLPs were used for genomic DNA extraction using Qiagen QIAamp DSP Virus Spin Kit (Qiagen, MD, U.S.A) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
-
bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted from 140 µl of each sample and also from the original virus dilutions with the “QIamp viral RNA extraction kit” (Qiagen) and eluted in 60 µl elution buffer according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... A single colony was picked with an inoculation loop and DNA extraction was performed on the QIAsymphony using the DSP Virus/Pathogen Mini Kit (Qiagen). Library preparation performed using Nextera XT (Illumina Inc. ...
-
bioRxiv - Microbiology 2024Quote: 200µL of lung of infectious cell supernatant (virus stock and HAE) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
-
bioRxiv - Microbiology 2021Quote: Recombinant-construct with N-terminal His-tag was purified through IMAC-based Ni-NTA (Qiagen). IPTG induced ...
-
bioRxiv - Immunology 2020Quote: ... The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen). After virus rescue was observed via plaque formation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... All recombinant DNA was isolated and purified through Miniprep kits according to manufacturer’s instructions (Qiagen). Sequences were confirmed by Sanger sequencing.
-
bioRxiv - Plant Biology 2022Quote: ... Size selection of libraries contained the desired insert size has been performed using SparQ beads (Qiagen). Libraries were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems) ...
-
bioRxiv - Cell Biology 2021Quote: ... UAS-CG9253(porthos)::FLAG::HA and srpHemo-GAL4 constructs using Effectene Tranfection Reagent (Qiagen, Hilden, Germany) following the manufacturer’s protocol (Table S3) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and S2R+-NPT005/MT-NLS-Cas13d-NLS-HA-T2A-GFP_CloneF11 cells were transfected using Effectene (Qiagen) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the respective TurboID-HA fusion constructs using Effectene™ (Qiagen, Hilden, Germany) following the manufacturer’s protocol and incubated for ∼24 hours in a petri-dish containing a coverslip ...
-
bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
-
bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
-
bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: A 200 μL aliquot of Copan UTM® was processed through the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Cat# 937036) following manufacturer’s instructions on the QIAsymphony SP instrument ...
-
bioRxiv - Molecular Biology 2020Quote: The RNA isolation was performed with 50 µl of the crude extract on the QIAsymphony with the DSP Virus/Pathogen Kit (Qiagen, #937055). The RT-PCR was performed using 5 µl of 85 µl eluate with TIB MolBiol Lightmix® MODULAR SARS AND WUHAN CoV E-Gene Kit ...
-
bioRxiv - Genomics 2020Quote: Viral RNA was extracted from nasopharyngeal swabs of patients with COVID-19 using the EZ1 DSP Virus Kit (Qiagen, Hilden, Germany), optimized for viral and bacterial nucleic acids extractions from human specimens using magnetic bead technology ...
-
bioRxiv - Microbiology 2021Quote: ... we collected the cell culture supernatants after 24 h of infection for viral RNA extraction with a QIAamp 96 Virus QIAcube HT Kit (Qiagen, 57731) and for viral RNA copy number detection in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid was extracted from the positive transformant using Qiagen® Plasmid Mini (Qiagen, USA) and sequenced (Advanced Innovative Trusted Products & Solutions ...
-
bioRxiv - Neuroscience 2023Quote: ... were used to analyse the levels of PrPC in vitro after has-miR-519a-3p Mimic (Qiagen) transfection ...
-
bioRxiv - Microbiology 2019Quote: ... viral RNA was extracted from the egg-isolate of the virus from the nasal swab using the QIAmp viral RNA mini-kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesized from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
-
Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomesbioRxiv - Genomics 2020Quote: RNA was extracted from nasopharyngeal swabs using the QIAamp Viral RNA Mini or the 213 EZ1 DSP Virus Kits (Qiagen, Hilden, Germany). All patients in this study tested positive for SARS-CoV-2 by RT-qPCR performed at Dubai Health Authority Hospitals ...
-
bioRxiv - Molecular Biology 2020Quote: ... The entire 200-µL reaction was used as input for ssAAV gDNA extraction using a MinElute Virus Spin Kit (Qiagen Cat#57704). 50-ng of ssDNA from each sample were bisulfite converted using the EZ Methylation Gold kit (Zymo Cat#D5005 ...
-
bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from virus library and ferret nasal wash samples using the QIAamp® Viral RNA mini kit (Qiagen, Germany). Next generation sequencing was carried out twice on the nasal washes ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA was purified using the QIAamp 96 virus QIAcube HT Kit and processed on the QIAcube robotic extraction platform (QIAgen, Hilden, Germany)
-
bioRxiv - Microbiology 2022Quote: ... A wild-type virus stock NL4-3 was prepared by transfection of the pNL4-3 plasmid (purified using the Qiagen Plasmid Maxikit) into HeLa cells ...
-
bioRxiv - Immunology 2019Quote: ... RNA was isolated from plasma samples using the QIAsymphony Virus/Bacteria Midi kit on the QIAsymphony SP automated sample preparation platform (Qiagen, Hilden, Germany). RNA was extracted manually if plasma volumes were limited ...
-
bioRxiv - Genomics 2020Quote: All 49 COVID-19 patients tested positive for SARS-CoV-2 by RT-qPCR using RNA extracted from nasopharyngeal swabs following the QIAamp Viral RNA Mini or the EZ1 DSP Virus Kits (Qiagen, Hilden, Germany). RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego ...
-
bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from 200 ul of oral swab material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA and DNA were extracted from fecal and urine samples using the QIAamp MinElute Virus Spin Kit (Qiagen, Valencia, CA, USA), but using 20μg of linear polyacrylamide (VWR ...
-
bioRxiv - Microbiology 2022Quote: Viral RNA was extracted from 200 ul of nasopharyngeal swab and BAL material using the Qiagen EZ1 DSP Virus kit on the automated EZ1 XL Advance instrument (Qiagen, Valencia, CA). Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was extracted from stock virus in allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen Inc., Valencia, CA, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... whereupon DNA was subsequently purified from 200 μl of the lysate using the QIAsymphony DSP Virus/Pathogen extraction kit (Qiagen Cat# 937036) on the QIAsymphony automated platform ...
-
bioRxiv - Molecular Biology 2023Quote: Total viral genomic DNA was extracted from 100 μL stored plasma samples with the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), according to manufacturers’ instructions ...
-
bioRxiv - Immunology 2024Quote: Virus RNA from in vitro or in vivo experiments was isolated using the QIAamp® Vira RNA Mini Kit (Qiagen, Germantown, MD) or the MagMAX-96 AI/ND viral RNA isolation kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid pENTR-CTDSP1 was extracted from positive colonies using the QIAprep Spin Miniprep Kit (Qiagen) and proper orientation of the cloned fragment was verified by PCR and DNA sequencing ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant plasmids were extracted from cell pellets using the QIAprep Spin Miniprep kit (Qiagen, Valencia, CA, USA). The sequence of the inserts was confirmed by Sanger sequencing (W ...
-
bioRxiv - Molecular Biology 2020Quote: ... pMK33/NLS-Cas13d-NLS-HA-T2A-GFP was transfected into S2R+-NPT005 cells (DGRC # 229) using Effectene (Qiagen) according to manufacturer’s protocol and selected in 200 ng/mL Hygromycin B (Calbiochem ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were co-transfected with 0.6 µg HA-ACKR3-FlAsH-CFP and 1.2 µg K44A dynamin per dish using the Effectene reagent (Qiagen) and following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
-
bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
-
bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
-
bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
-
bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
-
bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
-
bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...