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Citations for Qiagen :
501 - 550 of 927 citations for Recombinant Human Long Arg3 Insulin like Growth Factor I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... to extract RNA based on manufacturer instructions with an on-column DNase I digestion (Qiagen) for minimizing genomic DNA contamination ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was extracted using RNeasy Plant Mini Kit with on-column DNase I digestion (Qiagen). cRNA labeling and ATH1 hybridization were performed according to manufacturer’s instructions at GeneCore Facility ...
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bioRxiv - Plant Biology 2021Quote: ... and digested by DNAse I to eliminate DNA contamination according to the supplier’s recommendations (Qiagen). RNAseq analysis included 3 (exp1 and 3 ...
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bioRxiv - Plant Biology 2021Quote: ... All samples were treated with DNase I using a RNase-Free DNase Set (Qiagen, 79254) and quantified ...
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bioRxiv - Plant Biology 2019Quote: ... 10X diluted cDNA and SYBER Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen) was used to perform qRT-PCR on 7500 Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... On-column DNase I digestions were performed with the Qiagen RNase-free DNase Set (Qiagen). RNA was eluted with 200 μL RNAse-free water (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... An on-column DNase I digestion was carried out using RNase-free DNase set (QIAGEN). RNA was eluted in 30 μL of RNase-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by on-column incubation with DNase I to remove DNA (RNeasy Mini kit, Qiagen), and processing with a RiboZero rRNA removal kit (Epicentre/Illumina ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using the RNeasy kit with on-column DNase I digest (QIAGEN). 4 μg total RNA samples were reverse-transcribed in 40 μl reactions using the High-Capacity RNA-to-cDNA™ Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA contamination was removed in column using an RNase-free DNase I solution (Qiagen). RNA deep sequencing for SK36 and the manL mutant was carried out by Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was purified from organs with the RNeasy Mini Kit and DNase I (Qiagen)/cDNA was synthesized with iScript™ Reverse Transcriptase (Bio Rad) ...
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bioRxiv - Physiology 2022Quote: ... The washed pellet was resuspended in Buffer B with 300 μg/ml DNase I (QIAGEN) and 5 mM MgCl2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... by adding DNase I directly to the column from the RNase-free DNase kit (Qiagen) following kit protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Residual genomic DNA was eliminated from the total RNA fraction by DNase I treatment (Qiagen), according to the manufacturer’s protocol (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... samples were treated on-column with the Qiagen RNase-free DNase I set (Qiagen, 79254) according to the method described in appendix D of the Qiagen RNeasy mini kit manual (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... following the manufacturer’s instructions and treated twice with DNase I (1 U/μg RNA, Qiagen). The RNA concentration was quantified using the Nanodrop2000 (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... and RNA extracted by Qiagen RNeasy Plant Mini Kit with on-column DNA digestion with RNase-free DNase I (Qiagen).
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bioRxiv - Cancer Biology 2024Quote: ... and on-column DNase I treatment was performed using the RNase-Free DNase set (Qiagen). Tumor mRNA samples were submitted to the Montreal Clinical Research Institute (IRCM ...
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bioRxiv - Immunology 2024Quote: ... RNA was treated first with DNase I and purified with the miRNeasy mini kit (Qiagen), concentration measured by Nanodrop ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in growth medium for one hour before lysis and RNA purification with the RNeasy Mini Plus Kit (Qiagen). Control cells were treated identically except for the five-minute addition of dissociating enzyme.
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from 2 mL of overnight growth in BHI broth using the DNeasy Blood and Tissue kit (Qiagen). Genome sequencing of the two isolates was performed on a Miseq platform using Illumina technology ...
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bioRxiv - Microbiology 2019Quote: Strains were grown under agitating growth conditions in LB medium and total RNA isolation was performed using the RNeasy Mini kit (Qiagen). For removal of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from one 96-well microtiter plate of the 105-cell cryopreserved seawater inoculum cultures that exhibited growth using the DNEasy 96 Blood & Tissue Kit (Qiagen) in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and grown in serum-free medium for 24 hr and released into growth by addition of serum for 14 hr) by using RNA purification kit (QIAGEN). Purified RNAs were sequenced by GENEWIZ ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from triplicate samples at mid-log phase growth when succinate concentrations reached approximately 25 mM using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from cells recovered from the biofilms after 48 h of incubation on glass and two weeks of growth on the graphite electrode using the RNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Three individual colonies of WT and Pcu-shc::Δshc growth with and without 25 µM cumate were cultured to early exponential phase and total RNAs were extracted by Qiagen RNeasy Mini Kit as described previously (44) ...
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bioRxiv - Immunology 2021Quote: Conditioned medium of HEK293 cells producing recombinant sema3A fused with 6xHistidine tag in C-terminal was collected and purified using Ni-NTA agarose beads (QIAGEN). The protein activity was assessed using the cytoskeleton collapse assay (19 ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Molecular Biology 2019Quote: ... The expression construct was transformed into DH10MultiBacY cells and recombinant bacmid was purified with a QIAprep Spin Miniprep Kit (Qiagen). V0 and V1 virus generation was performed using SF9 cells as previously described 44 ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared via centrifugation and recombinant PfCen3-6xHis were purified from the soluble fraction using Ni-NTA agarose beads (QiaGen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... 6XHis tagged-Trx1 and Trr1 recombinant proteins were cloned in PET24 vectors and purified by column affinity onto Ni-NTA beads (Qiagen). The precursor was imported in 50 μg of the indicated yeast mitochondria in the presence of 2 mM ATP and 2.5 mM NADH at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Microbiology 2019Quote: ... The resulting 6xHis-tagged expression constructs were utilized to generate recombinant enzymes that were purified via Ni-NTA agarose (Qiagen). IDS enzyme assays were carried out in triplicate as previously described [45] ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Microbiology 2020Quote: ... cells were either left untreated or treated with 1000 IU/ml recombinant IFN-α treatment and incubated for 6 h or transfected overnight with polyI:C (10 μg/ml) using Polyfect (Qiagen). Luciferase assays were carried out ...
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bioRxiv - Molecular Biology 2023Quote: ... the large stocks of recombinant T/F LTR-pGL3 reporter plasmids were generated using Plasmid Maxi kit (Qiagen, Valencia, CA) for immediate use or longer storage at -80 °C.
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding the recombinant RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). On Day 0 ...
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bioRxiv - Biophysics 2023Quote: ... coli strain M15-[pREP4] (for transformation of recombinant plasmids) and RNAse (for protein purification) were purchased from Qiagen (Valencia, CA). All other chemicals were obtained from Sigma-Aldrich (St ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant protein was partially purified by affinity chromatography on nickel-nitrilotriacetic acid (Ni-NTA) agarose beads (Qiagen, http://www.qiagen.com). Purification steps were carried out as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Cell pellets from 6 mL samples collected at T1-T4 during growth of two biological replicates of the original XG-degrading culture were supplemented with RNAprotect Bacteria Reagent (Qiagen, USA) following the manufacturer’s instructions and kept at −80 °C until RNA extraction ...
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bioRxiv - Synthetic Biology 2022Quote: ... After overnight growth colonies were scraped into a collection bottle and plasmid pools were extracted using a plasmid maxiprep kit (Qiagen #12662). 2 smaller plates of LB with carbenicillin were also prepared with 1:20 diluted cells to count colonies and confirm transformation efficiency ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from initial and exponential growth phase cultures grown on different N sources using a DNeasy PowerSoil Pro DNA isolation kit (QIAGEN, Germany) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the RT2 Profiler Array “Type I Interferon Response” (Cat. No. 330231 PAMM-016ZA) (Qiagen, Hilden, Germany) was used ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenates were processed according to the RNeasy Mini Kit protocol and included a DNase I (Qiagen) treatment step to avoid contamination from genomic DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenates were processed according to the RNeasy Mini Kit protocol and included a DNase I (Qiagen) treatment step to avoid contamination from genomic DNA ...
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bioRxiv - Neuroscience 2021Quote: ... together with a genomic DNA eliminator column and a 30-minute incubation with DNAse I (Qiagen). Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μg of the isolated total RNA was treated with 30 U of DNase I (QIAGEN) and purified using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Microbiology 2019Quote: ... and the isolated RNA was treated with DNase I until the samples were DNA-free (Qiagen).