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Citations for Qiagen :
501 - 550 of 975 citations for Recombinant Human GH1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C overnight coupled with dialysis into buffer A.
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bioRxiv - Cancer Biology 2023Quote: ... before RNA isolation using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Next RNA and protein were digested and DNA was recovered by Qiagen PCR purification kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged protein was purified on a 1ml Ni-NTA column (Qiagen) prewashed in buffer A containing 500mM imidazole w/o β−mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then purified with Ni-NTA agarose beads (QIAGEN; 30210) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... His10 proteins were purified by using Ni-NTA agarose (QIAGEN, Catalog # 30230), followed by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The 6x-His-tagged proteins were captured using Ni-NTA Superflow (QIAGEN) and the His-tag was cleaved at 4 °C for 16-18 hours by human thrombin (Biopharm laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA agarose, Qiagen) and then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Biochemistry 2023Quote: ... for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins (2 hr ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: SacSm-containing protein sample was loaded on a Ni-NTA Agarose (Qiagen) column equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2024Quote: ... After the separation of nanodiscs and soluble proteins on Ni-NTA (Qiagen), the same proportions of unbound material and imidazole-eluted material were analyzed (Fig.S5) ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). Eluted protein was concentrated and passed over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tagged proteins were purified with HisBind NiNTA-agarose (Qiagen) according to the recommendations of the supplier ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Cancer Biology 2019Quote: ... and human BON1 cell line [8] were isolated with RNeasy Plus Universal Kits (Qiagen Cat no. 73404, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384 well plate). PCR plates were read the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... miScript miRNA PCR array of Human Neurological Development and Disease was performed using miScript SYBR green PCR kit (Qiagen). The PCR array contains 84 miRNAs which are differentially expressed during neuronal development and are responsible for progression of neurological diseases.
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: The human VCP cDNA was reverse transcribed from total RNA by using Omniscript RT kit (Qiagen Japan, Tokyo, Japan) extracted from A549 cells (RIKEN Cell Bank ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Immunology 2021Quote: ... followed by amplification and quantification using RT2 Profiler Human Innate and Adaptive Immune Response 96-well Array (Qiagen, 330231) with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequenced reads were mapped with the hg38 human genome and sequence analysis was done by the CLC genomics workbench (12.0.3, Qiagen Bioinformatics). Additionally ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from human tissue and cyst fluid using the QIAamp Mini kit (Qiagen, Hilden, Germany). Extractions were performed per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Raw FASTQ reads were aligned to the GRCh38 human genome using CLC Genomics Workbench version 22.0.2 software (Qiagen, USA). Differential gene expression was determined using the built-in tool in CLC Genomics workbench ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from human tissue derived organoids and neutrophils using the RNeasy® Plus Mini Kit (Qiagen, #74104) with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified using a Ni-NTA Superflow column (Qiagen, Netherlands, 30761) on a Äkta Express fast protein liquid chromatography system (FPLC ...
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bioRxiv - Microbiology 2019Quote: ... the fusion protein was purified using His-Bind columns (Qiagen, Venlo, The Netherlands) and analyzed by SDS-PAGE using gels containing 12% polyacrylamide ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transiently transfected with mammalian protein expressing plasmids using Effectene (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged proteins were purified on Ni-NTA agarose (Qiagen; catalog no. 30761) and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare ...
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bioRxiv - Genomics 2019Quote: ... RNA attached to purified protein complexes was isolated with the miRNeasy kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2020Quote: ... and the soluble protein fractions were purified by Ni-NTA resin (Qiagen, # 30230). The purified fraction was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen), amylose (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Whole DNA and proteins were extracted by the ALLprep Kit (Qiagen, Hilden, Germany), following manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen) following the instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Plant Biology 2020Quote: ... and the GST fusion proteins were purified by Glutathione HiCap Matrix columns (Qiagen) and eluted using 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...