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Citations for Qiagen :
601 - 650 of 2279 citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed using miScript II (218073; Qiagen) or miRCURY LNA (339371 ...
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bioRxiv - Microbiology 2023Quote: ... and worms were lysed in TissueLyser II (Qiagen) at 30 cycles sec-1 for 20 min ...
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bioRxiv - Developmental Biology 2024Quote: ... After bead beating with a TissueLyser II (QIAGEN) and 0.5 mm stainless steel beads (Next Advance ...
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bioRxiv - Microbiology 2022Quote: ... The solubilized membrane fraction was loaded on a column containing 0.5 mL Strep-tactin Superflow plus resin (50% suspension; Qiagen, Hilden, Germany) equilibrated with 4 mL of the Wash buffer ...
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bioRxiv - Genetics 2021Quote: ... Biotinylated RNA was released from beads using 100 mM DTT and cleaned using RNeasy MinElute Kit (QIAGEN 74204). Half of eluted RNA or 600 ng of depleted RNA was ligated to 5 µM of 50 adenylated ...
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bioRxiv - Genomics 2022Quote: ... and then PCR-amplified with biotinylated primers and pyrosequenced for allele quantification on a Pyromark Q24 system (Qiagen). The same PCR was done on no-reverse transcription control samples to confirm absence of genomic DNA contamination ...
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bioRxiv - Cancer Biology 2019Quote: ... The reverse transcription step exactly followed SMART-seq2 protocol with 5’-biotinylated TSO (Qiagen, primers see Table S9). The first round of 24-cycle PCR started with 10µl reverse transcription product ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2022Quote: ... Preparations of recombinant plasmids were conducted using the QIAprep Spin Miniprep Kit from QIAGEN, and then these plasmids were sequenced by BGI Group (Genome Sequencing Company) ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant MBP-Dnmt5(345-747)-6xHis was purified using Ni-NTA agarose resin (Qiagen) and measured by A280 (ε = 139,790 cm-1 M-1).
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant GST-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 66,030 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant MBP-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 89,590 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant TDP-43 was purified using the Ni-NTA SpinColumn purification kit (31014, Qiagen), essentially according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant plasmids from transformants were isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequence-verified via Sanger sequencing (Microsynth ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant plasmids from transformants were isolated using the QIAprep® Spin Miniprep Kit (Qiagen) and sequence verified via Sanger sequencing (Microsynth ...
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bioRxiv - Cell Biology 2023Quote: ... 20 mM imidazole and the recombinant V0c was purified over Ni-NTA beads (QIAGEN).
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bioRxiv - Microbiology 2023Quote: ... The recombinant plasmid was purified using QIAgen mini prep kit (cat no.27106, Qiagen) as per manufacture’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Recombinant plasmids were extracted by using the EndoFree Plasmid Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... The JAK3 PCR fragment was purified and cloned into the pQE-Tri System His-Strep2 vector (Qiagen). The final pQE-JAK3 construct was used to transfect GC using the Xfect transfection kit (Takara Bio ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Microbiology 2019Quote: ... soluble and insoluble fractions were separated by centrifugation and analyzed by SDS-PAGE and western blot using anti-Strep (Qiagen, Hilden, Germany) and anti-His (GE Healthcare ...
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bioRxiv - Developmental Biology 2019Quote: ... Biotinylated PCR products were mixed with streptavidin beads and converted to single strands using PyroMark Q96 Vacuum Workstation (Qiagen). Following annealing of sequencing primers ...
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bioRxiv - Cancer Biology 2020Quote: ... mito-roGFP and Myc-epitope-tagged murine Serpin E13,22,23 were purified with Plasmid Plus Midi columns (Qiagen, Inc., Germantown, MD) and administered to 6–8-week-old FVB mice via HDTVI (10 μg each ...
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bioRxiv - Neuroscience 2021Quote: ... 350 µl of 100% ethanol was added to the tube (“TRAP” fraction: enriched in transcriptome associated to EGFP-tagged ribosomes) and then loaded onto a RNeasy MinElute column (Qiagen). RNA was isolated using RNeasy Mini Kit (#74104 ...
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bioRxiv - Bioengineering 2021Quote: ... The histidine-tagged TEV and MBP were extracted from the solution using a Ni-NTA agarose resin (Qiagen, Hilden, Germany) as described in[20].
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bioRxiv - Biophysics 2020Quote: ... Cells were transiently transfected with cDNA encoding the mouse Piezo1 channel or its mutants tagged with GFP on its N-terminus in the pCDNA3 vector using the Effectene reagent (QIAGEN). Cells were then trypsinized and re-plated on poly-D-lysine-coated round coverslips 24 hours after transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 [60] and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 (Klueg et al., 2002) and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: Immunoprecipitation against HA-tagged exosomes was performed using Abcam Immunoprecipitation protocol on pan-exosomes isolated using the exoEasy kit (Qiagen). Protein G beads (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Immunology 2020Quote: ... The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen). After virus rescue was observed via plaque formation ...
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bioRxiv - Synthetic Biology 2023Quote: ... All recombinant DNA was isolated and purified through Miniprep kits according to manufacturer’s instructions (Qiagen). Sequences were confirmed by Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA synthesized with miScript II RT kit (Qiagen) using miScript HiFlex buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Leaf tissue was ground using the TissueLyser II (Qiagen) and DNA was extracted using the Invisorb DNA Plant HTS 96 Kit (cat 7037300400 ...
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bioRxiv - Neuroscience 2019Quote: ... purified (Qiaex II gel extraction kit, Qiagen, Hilden, Germany) and ligated into pGEM-T (Promega protocol) ...
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bioRxiv - Microbiology 2020Quote: ... Frozen seedlings were ground using a TissueLyzer II (Qiagen), then homogenized in a buffer containing 400 μL of Z6-buffer ...
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bioRxiv - Genetics 2020Quote: ... were ground into fine powder with TissueLyser II (QIAGEN). The powder was washed by adding 0.9 mL of Extraction Buffer I (Additional File 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor samples were homogenized with Tissue Lyser II (Qiagen) and digested with protease K in a concentration of 20 mg/ml (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The microtubes were loaded in the TissueLyser II (Qiagen) support plates (24 × 2 ...
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bioRxiv - Neuroscience 2021Quote: ... by using a rotor-stator homogenizer (TissueRutpor II, Qiagen). We purified RNA with RNeasy Micro columns (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then homogenized using a TissueLyser II (Qiagen) at 25Hz for 2m ...
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bioRxiv - Plant Biology 2021Quote: ... Leaf material was disrupted using the TissueLyser II (Qiagen). On-column DNase digestion was performed using the RNase Free DNase Set (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... or the miSCRIPT II RT kit (Qiagen, Valencia, CA). Primers to NFKB1 and 18s were designed using the Roche Universal Probe Library assay design center and qPCR was performed using Quanta PerfeCTa Supermix ...
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bioRxiv - Physiology 2021Quote: ... tissue was homogenized with TissueLyser II (Qiagen, Hilden, Germany), and total RNA was extracted with TRIzol (Invitrogen ...