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Citations for Qiagen :
251 - 300 of 1879 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... cultured and then isolated using a Hi-speed Midiprep kit (Qiagen). Expression was first examined by transfection of N2A cells in vitro ...
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bioRxiv - Cell Biology 2021Quote: ... and His6- Vps4 was detected using Penta-His Antibody (Qiagen, Germany) per manufacturer instruction ...
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bioRxiv - Microbiology 2022Quote: ... Antibodies against His-tag and S-tag were purchased from Qiagen and GenScript respectively ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Microbiology 2019Quote: His Tag-Alexa 647 (5 ug/mL) (Qiagen, catalog no. 35370)
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bioRxiv - Cell Biology 2021Quote: ... was from List Biological Laboratories and the mouse monoclonal antibody against the His-tag from Qiagen.
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bioRxiv - Biophysics 2022Quote: The following anti-His fluorophore-conjugated antibodies were purchased from Qiagen and used in UV-Bind assays for His-tagged proteins ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Microbiology 2021Quote: Total RNAs from HC69 or HIV-infected IMG cells with different treatments were isolated by using the RNeasy Plus Mini kit from Qiagen (Cat#74134). The purified total RNAs were converted to first-strand cDNAs by using a reverse transcription kit (Bio-Rad ...
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bioRxiv - Genetics 2020Quote: HCV and HIV RNA were extracted from 200 μL plasma using a QIA amp® Viral RNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacture’s instruction and 50 μL of eluted RNA was stored at −70 °C until use ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Electroblotted membranes were incubated with penta-His antibody (Qiagen, Valencia, CA, USA). The antigen–antibody complex was visualized with alkaline phosphatase-linked to anti-rabbit IgG followed by staining with 5-bromo-4-chloroindol-2-yl phosphate and nitro blue tetrazolium [36].
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The primary and secondary antibodies used were anti-penta His (Qiagen #34660) and goat anti-mouse IgG (LiCOR #926-68070 ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Microbiology 2019Quote: ... His-cyAbrB1 was purified with the Ni-NTA Fast Start kit (Qiagen). The elution fractions containing the purified protein were loaded onto a PD MidiTrap G-25 column (GE healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... His-NaERF2-like were expressed and purified with Ni-NTA agarose (QIAGEN). Biotin labeled probe EM13 (5’-tagattATCTaattctact-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-TRIM39 proteins were purified on Ni-NTA agarose beads (Qiagen, #1018244) and then eluted in a buffer containing 0.5 M imidazole before dialysis in PBS.
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bioRxiv - Cell Biology 2022Quote: ... and 6×His-Flag-Ub was conducted using Ni-NTA agarose (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... His-tag proteins were purified using a Ni-NTA purification system (Qiagen) by following the specification of the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... a conjugate of mouse monoclonal penta-His antibody and horseradish peroxidase (Qiagen) was used ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transiently transfected with HA-tagged RILP or ORP1L constructs using Effectine (Qiagen), harvested at 24 hours post-transfection and fixed in 3.7% formaldehyde in PBS for 15-20 min ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged Lem27 or its truncation mutants was purified using Ni2+-NTA columns (Qiagen). After washing with a buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Microbiology 2019Quote: ... the fusion protein was purified using His-Bind columns (Qiagen, Venlo, The Netherlands) and analyzed by SDS-PAGE using gels containing 12% polyacrylamide ...
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bioRxiv - Microbiology 2020Quote: ... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP was first isolated using Ni-NTA beads (Qiagen, Valencia, CA) using a buffer with an imidazole gradient (20 mM Bis-Tris ...
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Biochemistry 2021Quote: Assay mixes consisted of 25 nM ALEXA488-conjugated penta-His antibody (Qiagen # 35310), 50 μM DTT ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Target protein was recovered and purified with a His-Trap affinity column (QIAGEN) according to manufacturers’ protocols ...
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bioRxiv - Cancer Biology 2024Quote: rEDA was produced intracellularly using the pQE-30 His tag purification system (Qiagen) in E ...
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bioRxiv - Molecular Biology 2019Quote: ... His6- and GST-tagged proteins were affinity-purified in batch on Ni-NTA agarose (Qiagen) and glutathione Sepharose 4 Fast Flow (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged AC was secreted into the medium and purified by nickel affinity chromatography (Qiagen) with elution in 100 mM citrate pH 4.0.
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bioRxiv - Microbiology 2020Quote: ... The histidine-tagged proteins MprA and MprA* were purified using Ni-NTA Agarose beads (Qiagen) using standardized protocol(Chadha et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant lysates were purified on Ni-NTA agarose beads (for His6-tagged proteins, Qiagen) or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 6xHis tagged proteins were purified from the clarified lysates by using Ni-NTA beads (Qiagen), and incubated with end-over-end rotation overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...