Labshake search
Citations for Qiagen :
151 - 200 of 1566 citations for Rat TUT1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were purified using QIAquick Spin Miniprep or Plasmid Plus Midi kits (Qiagen) and digest verified prior to use ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was prepared using HiSpeed Plasmid Maxi Kit (QIAGEN, Germantown, MD, USA). DNA purity and concentration were identified using NanoDrop (ThermoFisher SCIENTIFIC ...
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bioRxiv - Bioengineering 2022Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Microbiology 2022Quote: ... Bulk preparation of all plasmids was performed using the plasmid midiprep kit (Qiagen). Cells (1×105 ...
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bioRxiv - Biochemistry 2022Quote: ... and the plasmid was purified using a Qiagen Plasmid Midi Kit (Qiagen 12943).
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bioRxiv - Microbiology 2020Quote: ... The plasmids with inserts were purified by using a Plasmid Midi Kit (Qiagen). The plasmid concentration was determined by photospectroscopy at 260 nm using the BioDrop Duo™ spectrophotometer (BioDrop ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was prepared using the Plasmid Midi kit (Qiagen, Montréal, QC, Canada). HEK293T cells were seeded into 10 cm culture dishes and transfected the next day using polyethyleneimine (PolyScience ...
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bioRxiv - Immunology 2022Quote: ... The plasmid DNA was prepared with an Endofree Plasmid Maxi Kit (Qiagen, 12362). For the validation library ...
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bioRxiv - Immunology 2022Quote: ... and endotoxin-free plasmid stocks were prepared (Qiagen EndoFree Plasmid Maxi Kit; #12362). RV was generated for each construct as previously described (60).
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bioRxiv - Genetics 2019Quote: ... Colonies were harvested and plasmids were extracted using a Plasmid Maxiprep kit (Qiagen).
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bioRxiv - Cancer Biology 2019Quote: ... All construct plasmids were prepared using the Plasmid Plus Midi Kit (Qiagen, 12943). To generate lentivirus for each construct ...
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bioRxiv - Genetics 2019Quote: ... The sgRNA library plasmids were extracted with a Plasmid Plus Maxi Kit (QIAGEN). The library plasmids were amplified using PrimeSTAR GXL DNA Polymerase (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid DNA for transfections was prepared using a HiSpeed Plasmid Midi Kit (Qiagen). Vero E6 cells were seeded into 24 well trays at 105 cells per well ...
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bioRxiv - Immunology 2021Quote: ... The plasmids were extracted with 12 columns of EndoFree plasmid Maxi kit (Qiagen). To prepare the virus library ...
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bioRxiv - Evolutionary Biology 2021Quote: Plasmids from UCI38 were extracted using QIAfilter Plasmid Midi Kit (Qiagen, Cat.# 12243). Extracted plasmids were then transformed into other clinical isolates and MGH21Δcas through electroporation ...
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bioRxiv - Genomics 2021Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids from Lactobacillus were prepared using QIAGEN® Plasmid Midi Kit (Qiagen, Germany) following supplier’s direction with a few modifications to cell wall lysis ...
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bioRxiv - Immunology 2021Quote: ... Library plasmid has been isolated using the Qiagen Plasmid Plus MaxiKit (Qiagen 12963) and sequenced for sgRNA representation as described under “Genome-wide CRISPRa and CRISPRi screens”.
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bioRxiv - Genetics 2021Quote: ... Plasmid DNA was extracted using the QIAGEN Plasmid Plus 96 Kit (Qiagen 16181) and guide cloning confirmed by Sanger sequencing ...
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bioRxiv - Genetics 2020Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Genetics 2022Quote: ... Plasmid DNA for the retrotransposition assays was obtained using Plasmid Maxi kit (Qiagen). The absence of mutations was verified by capillary sequencing ...
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bioRxiv - Physiology 2022Quote: ... then amplified and purified the plasmids using an EndoFree Plasmid Maxi Kit (Qiagen). By our established methods (26) ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNAs were purified using QIAfilter Plasmid kits (Midi pre kit, Qiagen #12243) following manufacturer protocol ...
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bioRxiv - Systems Biology 2022Quote: ... and plasmid pools were extracted using the Qiagen Plasmid Maxi Kit (Qiagen #12162). To assess library quality and representation bias ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids with inserts were purified by using a Plasmid Midi Kit (Qiagen). Plasmid concentration was determined by photospectroscopy at 260 nm using the BioDrop DuoTM spectrophotometer (BioDrop ...
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bioRxiv - Genomics 2023Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid DNA was prepared using the Plasmid Plus Midi kit (Qiagen, Hilden, Germany) except that three additional wash steps were performed before DNA elution from the column ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids for the transfection were purified using an endotoxin-free Plasmid Kit (Qiagen). Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cultures were pelleted and plasmids were purified using a Plasmid Maxiprep Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA plasmid was extracted from positive colonies by EndoFree Plasmid Maxi kit (QIAGEN) and double-digested with HindIII (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA cloning and purification was performed using QIAGEN Plasmid Midi Kit (Qiagen) according to manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Genomics 2023Quote: ... The plasmid library was extracted using QIAGEN Plasmid Plus Maxi Kit (QIAGEN 12963). The product was further purified using ethanol precipitation and resuspended in pure H2O ...
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bioRxiv - Cell Biology 2023Quote: ... All plasmids used for transfection were purified using the Plasmid Midi kit (Qiagen). Cells were harvested 36-48 h post-transfection for further analyses.
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted using the QIAGEN Plasmid Maxi Kit (Qiagen, cat.no. 12165). All cloned sequences were validated by Sanger sequencing.
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bioRxiv - Biophysics 2023Quote: ... plasmid extraction was carried out using the Plasmid Plus Midi Kit (QIAGEN®) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Colonies were scraped and plasmid DNA (pDNA) was prepared (HiSpeed Plasmid Maxi, Qiagen). To confirm library representation and distribution ...
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bioRxiv - Cell Biology 2023Quote: ... The PB-TAC plasmid library was extracted using the Plasmid Midi Kit (Qiagen).
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bioRxiv - Genomics 2024Quote: ... Plasmid libraries were then purified with a Plasmid Plus Midi Kit (Qiagen 12943) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: All the plasmids were prepared endotoxin free using the EndoFree Plasmid Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg plasmid DNA (1 μg/μL; prepared by QIAGEN Plasmid Midi Kit) in 30 μL MMG solution was used to transform 200 μL protoplasts by gently mixing with 230μL 40% PEG transformation buffer ...
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bioRxiv - Biochemistry 2020Quote: Total RNAs were extracted from Wildtype MIN6 and MIN6 stably expressing shRNAs (non-specific/NS, C, D) using RNeasy kit (QIAGEN, Germantown, MD) and cDNAs were prepared using qScript cDNA SuperMix (Quantabio ...
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bioRxiv - Cancer Biology 2021Quote: ... The plasmids were purified using QIAquick Spin Miniprep or Plasmid Plus Midi kits (Qiagen) and verified by Sanger sequencing (Genewiz ...
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bioRxiv - Genetics 2021Quote: ... All plasmids used for mammalian cell experiments were purified using plasmid Miniprep kits (Qiagen).
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bioRxiv - Evolutionary Biology 2021Quote: ... Plasmid mutant libraries were purified from the bacteria colonies using Plasmid Midi Kit (Qiagen).
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bioRxiv - Genomics 2020Quote: ... bacteria were harvested and plasmid DNA purified with an EndoFree Plasmid Maxi kit (Qiagen). Dilution plates of electroporated cells confirmed a colony count of >100X relative to the size of the gRNA library ...
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bioRxiv - Genomics 2020Quote: ... the colonies were scraped and plasmids were purified using a Plasmid Maxiprep kit (Qiagen).
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bioRxiv - Systems Biology 2019Quote: ... and plasmid DNA extracted using a QIAfilter Plasmid Maxi Prep kit (Qiagen, Valencia, CA). DNA was standardized to 500 ng/µL ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were purified using a Midiprep plasmid DNA purification kit (Cat. No. 12143; Qiagen). All constructs were verified via DNA sequence analyses.
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bioRxiv - Genomics 2020Quote: ... pooled and the plasmid library was extracted using a Plasmid Plus Mega Kit (Qiagen). Transfection of U2OS-AR or U2OS-GR cells ...