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Citations for Qiagen :
401 - 450 of 10000+ citations for Rat Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Genomics 2021Quote: ... and grown at 30°C or 37°C for the plasmid DNA preparation (Qiagen miniprep). The resulting plasmids were sequence-verified using Sanger sequencing (Genewiz).
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in sphere-forming cultures for 2 weeks prior to RNA collection (RNAeasy Kit, Qiagen). TGFB1 was replenished when placing mOSE cells in sphere-forming conditions.
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bioRxiv - Cell Biology 2019Quote: ... At least 2×102 cells were lysed and RNA was extracted with the RNAeasy Mini Kit (Qiagen) following manufacturer specifications ...
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bioRxiv - Microbiology 2022Quote: ... 2×109 bacteria were pelleted and DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen). The optional RNase step in the protocol was performed to remove contaminating RNA ...
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bioRxiv - Microbiology 2019Quote: ... The PCR product was purified from a 2% TAE-agarose gel with a Gel Extraction Kit (Qiagen) and phosphorylated for 30 minutes at 37 °C with 4U of T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting amplicon pool was further purified from a 2% agarose gel (QIAquick Gel extraction Kit Qiagen) with GelRed staining and eluted in TE (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1-2 µg of RNA was used for the cDNA synthesis (Omniscript RT Kit (Qiagen, 205111)) ...
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bioRxiv - Molecular Biology 2022Quote: 2 x 106 cells were harvested and RNA was purified using Qiagen RNeasy Mini Kit (Qiagen, #74104) using manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell supernatant was extracted using the QIAamp viral RNA mini kit (Qiagen). A two-step qRT-PCR was used to detect viral RNA released in the cell supernatant ...
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bioRxiv - Developmental Biology 2022Quote: ... and 2 μg of extracted total RNA was reverse-transcribed using the Omniscript RT Kit (205111, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genetics 2021Quote: Total RNA was prepared from AKR and DBA/2 BMDM using miRNeasy Mini Kit (Qiagen; # ID 217004) with on-column DNA digestion according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... DNA was isolated from 1-2 mm tail tissue using the DNeasy Blood and Tissue Kit (Qiagen) following the protocol for isolation of DNA from animal tissues and was eluted in 100 µl H2O ...
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bioRxiv - Paleontology 2019Quote: Human 2 and 3: DNA was extracted from bones using QIAamp® DNA Investigator kit (56504, Qiagen). Bones were thoroughly washed (X5 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 replication was quantified via RT-qPCR using the QuantiTect Multiplex RT-qPCR Kit (Qiagen) with a Rotor Gene Q cycler (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 S mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... Products between 200bp-600bp were purified from 2% agarose gels run in 0.5XTAE using QIAquick kits (Qiagen), and excess adaptors were removed by magnetic beads (HighPrep PCR ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA was extracted from the PBMC obtained from 2 alpacas using standard RNA extraction kit (Qiagen). Recovered mRNA was retrotranscribed to cDNA by using Supercript IV (ThermoFischer ...
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bioRxiv - Cell Biology 2022Quote: Amplicons were separated on 2% agarose-TAE gels and purified using the QIAquick Gel Extraction Kit (Qiagen). Amplicons were sequenced at Quintarabio ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from 2 biological replicates of fecal pellets using DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from pools of 2-3 organoids using RNeasy Plus Mini Kit (Qiagen, #74134) according to the manufacturer’s protocols ...
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bioRxiv - Genetics 2024Quote: ... 2 or 4 days and genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen). Bisulfite converted DNA libraries were prepared using the Accel-NGS Methyl-Seq DNA library kit (SWIFT BIOSCIENCES) ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 2-5 million flash frozen cells using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... 2 g of soil were used for RNA extraction using the RNeasy PowerSoil Kit (Qiagen, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Luciferase reporter constructs were either mock-treated or methylated in vitro with SssI CpG methyltransferase for 4 h at 37 °C and purified with the QIAquick Purification Kit (QIAGEN 28704). Reporter plasmid (500 ng ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The reaction was incubated at 37°C for 30 min and purified using a MinElute Reaction Cleanup Kit (Qiagen, Germantown, MD). The PCR reaction consisted of 30 μl transposed DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Bacterial cells were pelleted by centrifugation at 6,000 RCF at 4°C for 15 minutes and plasmid DNA was extracted using a Qiagen Plasmid Plus Midi Kit (Qiagen #12943). To estimate the total number of unique barcodes present in the plasmid pool ...
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bioRxiv - Genomics 2019Quote: ... The fresh blood sample was immediately brought to the laboratory at 4 °C and genomic DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: We homogenized tongue or ear tissue (stored at −20°C) and extracted genomic DNA using QIAamp Fast Tissue kits (Qiagen, Inc), with verification via gel electrophoresis (2% agarose) ...
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bioRxiv - Genomics 2021Quote: ... The resultant nuclei were then incubated for 30 min at 37°C with an in-house made Tn5 enzyme and purified with the Qiagen MinElute kit (Qiagen, 28004). PCR reactions for each replicate were performed with 15 cycles using Ad1F and Ad2.2R primers and NEBNext High-Fidelity 2X PCR Master Mix (New England Labs Cat #M0541) ...
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bioRxiv - Molecular Biology 2021Quote: ... the PCR products were held at 4°C until PCR purification using the QIAquick PCR purification kit (#28106, Qiagen, Hilden, Germany). DNA concentrations were determined using NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... harvested and stored at −80°C in RLT buffer with β-mercaptoethanol from the RNeasy Mini prep Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions until cDNA synthesis for RT2 Profiler PCR array ...
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bioRxiv - Microbiology 2021Quote: ... Fecal pellets were snapfrozen and stored at −80°C until DNA extraction with the DNeasy Powersoil kit by Qiagen (Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Transposition was performed for 30 minutes at 37°C after which the transposed DNA was purified using MinElute PCR Purification Kit (Qiagen, 28004) and eluted in 10 µl of elution buffer (EB ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Microbiology 2019Quote: ... the supernatant was removed with a micropipet and 350 µL of 4°C Buffer RLT Plus (from Qiagen RNeasy Mini Kit) was added to the pellet to collect the RNA ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2019Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80°C until clean up using a kit (Qiagen 154025593). cDNA was generated using 11 rounds of amplification with 10 ng RNA input.
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Neuroscience 2021Quote: ... Buffer RLT containing immunoprecipitated RNA was then eluted and stored at −80 °C until clean up using a kit (Qiagen 74004). cDNA was generated using 11 rounds of amplification with 10 ng RNA input ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The right pectoral fin was removed and stored in 95% ethanol at −20 °C prior to subsequent DNA extraction using QIAamp Fast DNA Tissue kits (Qiagen, Inc.) following the manufacturer’s protocols.
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bioRxiv - Genomics 2022Quote: ... After overnight incubation at 37 °C colonies were picked for mini-prep cultures and mini-preps were performed using the Qiaprep® Miniprep kit (Qiagen). The insert in the resulting plasmid was sanger sequenced (Source Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
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bioRxiv - Cell Biology 2022Quote: ... Islets were pelleted and stored at −80°C until DNA extraction using the Blood/Tissue DNeasy kit (Qiagen; Germantown, MD, USA) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were incubated at 37°C for 30 min and then purified using the MinElute Reaction Cleanup kit (Qiagen Cat #28204) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... Liver samples were stored at -140°C and prepared as follows: total RNA was extracted using miRNeasy kit (Qiagen; Hilden, Germany). Libraries were prepared using 10 ng/μl with Truseq Stranded RNA ...