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Citations for Qiagen :
101 - 150 of 10000+ citations for Rat Isocitrate Dehydrogenase 1 Soluble IDH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... total RNA was isolated from the hippocampi of IBU-treated and untreated transgenic rats using the RNeasy Mini Kit (Qiagen cat# 74104). The integrity of total RNA was examined by the Agilent 4200 TapeStation System ...
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bioRxiv - Microbiology 2019Quote: ... Cignal EGR-1 reporter kit (Qiagen) was transfected in HEK293T following manufacturer protocol ...
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bioRxiv - Neuroscience 2020Quote: ... rat MPG were harvested and kept in RNAlater (Qiagen) immediately after cavernosometry ...
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bioRxiv - Cell Biology 2019Quote: ... then mixed with BioMag goat-rat IgG beads (Qiagen) and Ter119+ cells were depleted using a Dynal magnet (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Next the lysate was clarified by centrifugation and the soluble fraction was incubated with equilibrated Ni-NTA resin (Qiagen cat#4561) for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble 6His-tagged proteins were purified from the supernatant by affinity chromatography using Ni2+-NTA agarose resin (Qiagen, Hilden, Germany). After several washing steps ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: Viral RNA was extracted from clarified cell lysates or samples obtained from cotton rats as described [55] by using the Mini Viral RNA Kit (Qiagen Sciences, Germantown, Maryland) and automated platform QIAcube (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... DNA from a male adult SHR/OlaIpcv rat was extracted from brain tissue using DNEasy Blood and Tissue Kit (Qiagen, Cat no. 69504) and amplified using Phusion High-Fidelity PCR Kit (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... and the 6xHis-SUMO tagged proteins were purified from the soluble protein fraction after centrifugation using an Ni2+-NTA Superflow column (Qiagen, Venlo, Netherlands). Next ...
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bioRxiv - Microbiology 2019Quote: ... soluble and insoluble fractions were separated by centrifugation and analyzed by SDS-PAGE and western blot using anti-Strep (Qiagen, Hilden, Germany) and anti-His (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting supernatant containing soluble LCC was subjected to purification by immobilized metal ion chromatography (IMAC) using Ni-NTA Superflow (Qiagen, Hilden, Germany). Protein elution using 250 mM imidazole was then dialyzed against 100 mM potassium phosphate buffer (pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... His-tag fusions were purified by incubating the bacterial soluble fraction with pre-equilibrated Ni-NTA agarose bead slurry (Qiagen, Hilden, Germany) for 1 hour at 4°C and eluting with 500 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... 1×106 cells using RNeasy Kit (Qiagen), followed by DNase digestion using TURBO DNase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1) DNeasy PowerLyzer PowerSoil Kit (QIAGEN®), 2 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... containing 1 × Master Mix (Qiagen Multiplex Kit), 0.4 μg/μL of BSA ...
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bioRxiv - Genetics 2020Quote: ... Whole brain tissue from 3-grouped rats containing SNpc were coronal sectioned and the genomic DNAs were extracted using a DNeasy® Blood & Tissue Kit (QIAGEN, Valencia, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... followed by magnetic depletion using goat anti-rat beads (QIAGEN). For adoptive transfer experiments ...
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Immune profiling in M. tuberculosis infection enables stratification of patients with active diseasebioRxiv - Immunology 2019Quote: Supernatants from QFT and TruC tubes were analyzed for IFNγ by standard ELISA (Qiagen) and values were expressed in IU/mL ...
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bioRxiv - Cell Biology 2020Quote: ... cells were mixed with anti-rat Ig magnetic beads (Qiagen BioMag), incubated for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated with anti-rat Ig magnetic beads (Qiagen BioMag), and subjected to magnetic separation ...
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bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
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bioRxiv - Cancer Biology 2022Quote: ... three kits were used: (1) Qiagen DNeasy Blood & Tissue Kits (Qiagen, cat. no. 69504) for DNA extraction and (2 ...
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bioRxiv - Genomics 2023Quote: ... gracilis (1 male and 1 female) using the Rneasy Mini Plant Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and native rat tissue samples were homogenized in lysis Buffer RLT (Qiagen). Total RNA was isolated using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with goat anti-rat beads (QIAGEN, cat. no. 310107). For intracellular staining ...
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bioRxiv - Physiology 2021Quote: 1 μg purified extracted RNA (RNeasy Mini Kit, Qiagen) was reverse transcribed using qScript Reverse Transcriptase (Quanta Biosciences ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL RTL buffer (Qiagen RNeasy Kit) and lysed by bead beating (2 × 1 min ...
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bioRxiv - Cell Biology 2019Quote: ... The siRNA targeting rat Psmc1 included Rn_RGD:621097_1 FlexiTube siRNA (Qiagen, Cat# SI02002427) and Rn_RGD:621097_2 FlexiTube siRNA (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from cultured rat embryonic striatal neurons following stimulation (RNeasy, Qiagen) and submitted to the Genomic scorelab at the Heflin Center for Genomic Sciences at the University of Alabama at Birmingham for library preparation as previously described17,47 ...
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bioRxiv - Neuroscience 2021Quote: ... A customized RT2 Profiler PCR array for rat angiogenesis markers (PARN 042Z, 330231, Qiagen) containing SYBR Green RT PCR assays for 84 genes of interest ...
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bioRxiv - Cell Biology 2022Quote: Alox12b and Tgm1 shRNA knockdown in rat epidermal keratinocytes using SureSilencing shRNA plasmids (Qiagen) has been previously described (O’Shaughnessy et al ...
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bioRxiv - Cell Biology 2022Quote: To synthesize cDNA from 1 μg RNA the Quantitect kit (Qiagen) was used according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RNA (1 µg) was extracted with the RNeasy Kit (QIAGEN, 74106) and treated with DNase I following the manufacturer’s instructions (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... PCR#1 product was purified using Minielute PCR purification kit (Qiagen) and a second round of PCR amplification was performed with gene-specific primers R2 and forward F2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... from 1 x 107 cells using the RNeasy Mini Kit (Qiagen) and the TruSeq Stranded mRNA kit (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 colonies using a DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). We used DNeasy Blood & Tissue Kits (QIAGEN ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
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bioRxiv - Biochemistry 2024Quote: ... ∼8 ml PCR product (azide-DNA:2xbiotin-DNA = 1:1) was purified using HiSpeed Plasmid Maxi kit (Qiagen) in 1 ml NaHCO3 (pH 8.3) ...
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bioRxiv - Cell Biology 2020Quote: RNA was extracted from 1 million cells with RNeasy mini Kit (Qiagen) following the manufacturer’s instructions and with DNase treatment on column ...
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bioRxiv - Plant Biology 2021Quote: ... Genomic DNA (1 μg) isolated using a DNeasy plant mini kit (Qiagen) was used for library construction ...
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bioRxiv - Plant Biology 2021Quote: ... nks1-1 and nks1-2 using RNeasy Plant mini kit (74904, QIAGEN). cDNA was synthesized from 1 μg total RNA using iScript cDNA synthesis kit (1706691 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA (1 μg) was was extracted using miRNeasy Mini Kit (Qiagen) and applied to either miR-based or mRNA based reverse transcription ...
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bioRxiv - Microbiology 2020Quote: ... and IFN-stimulated DF-1 and CEF using the RNeasy kit (Qiagen) according to the manufacturer’s instructions as previously described48 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg RNA was reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg RNA was reverse transcribed using miScript II RT kit (Qiagen) as per protocol before qPCR using miScript SYBR Green kit (Qiagen ...