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Citations for Qiagen :
1 - 50 of 10000+ citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... with beta-actin as a reference gene (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from ≈10 mg of MCT rat lung tissues using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen) following to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT (with 143mM beta-mercaptoethanol) and RNA purified using the RNeasy kit (Qiagen). 0.8-2μg of RNA were reverse transcribed using the High-Capacity cDNA reverse transcription kit and quantitative real-time PCR was carried out with the ViiA 7 Real-time PCR system (both Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... + 5% beta-mercaptoethanol using a bead mill (Qiagen). Samples were heated at 95° for 5 minutes to denature proteins ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted in RLT buffer supplemented with beta-mercaptoethanol and processed with the RNeasy Micro Kit (QIAGEN) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RLT buffer with Beta-mercaptoethanol and RNA was extracted using Qiagen RNEasy kit (Qiagen; #74134). Extracted RNA was then converted to cDNA using the one-step qSCRIPT cDNA solution ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized in Buffer RLT+ beta-mercaptoethanol (Qiagen). Tissue homogenate was then centrifuged through a QIAshredder homogenizer (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... and beta-mercaptoethanol using the TissueLyser II (Qiagen, Australia). DNA was extracted using the AllPrep DNA/RNA Mini Kit following the manufacturer guidelines (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was isolated from 1×106 BMMCs that were untreated or treated with IgE receptor crosslinking using the RNeasy mini kit (Qiagen). RNA was treated with DNase I according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... All plasmids were verified by sequencing of the full receptor cDNA and purified with an EndoFree Plasmid Maxi Kit (Qiagen). cDNA sequences of all GPCR variants cloned in pcDNA3.1 are summarized in Data S2.
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bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNAs were isolated from cerebral cortices (P15 rats) using Mini RNeasy kit (Qiagen) then converted to cDNA using 1 µg RNA and a QuantiTect Reverse Transcription kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
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bioRxiv - Immunology 2020Quote: A RT2 profiler array for mouse inflammatory cytokines and receptors (Qiagen, USA) was used according to manufacturer recommendations to measure the expression of 84 inflammatory genes (Table S1) ...
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bioRxiv - Neuroscience 2020Quote: Total hippocampus RNA was isolated from 4-month olds rats using RNeasy Lipid Tissue Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... males n=11) and Ob (females n=10, males n=10) offspring using a commercially available kit (RNeasy Plus mini Kit, Qiagen) then reverse transcribed to cDNA (High Capacity cDNA Reverse Transcription kit ...
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bioRxiv - Neuroscience 2022Quote: We isolated genomic DNA from rat tail tips using Qiagen genomic DNA extraction kit (Qiagen Inc., USA). We performed genotyping via PCR ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
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bioRxiv - Immunology 2021Quote: Total DNA was extracted from Wistar rat tissues samples using the DNeasy Blood & Tissue Kit (Qiagen, Germany) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from rat lower esophageal tissues using the RNeasy Fibrous Tissue Kit (Qiagen, Germantown, MD). Each sample was homogenized in 400 µL of Buffer RLT with beta-mercaptoethanol for 3 – 10 second pulses with the homogenizer (Pro-Scientific Inc. ...
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bioRxiv - Developmental Biology 2023Quote: ... genomic DNA was extracted from rat PSC cell pellets using the DNeasy Blood and Tissue kit (Qiagen) and PCR was performed using the Platinum PCR SuperMix High-Fidelity kit (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
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bioRxiv - Immunology 2023Quote: ... ∼1000 macrophages were sorted into 75µL of RLT buffer (Qiagen, containing 1% beta-mercaptoethanol), vortexed for 1 min and immediately frozen (–80°C) ...
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bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... mRNA was prepared from 10 dissected brains or 10 testes using the RNeasy kit (Qiagen). cDNA was then generated using the QuantiTect Reverse Transcription kit (Qiagen).
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bioRxiv - Immunology 2022Quote: ... For RT2 Profiler PCR Array Human Interferons and Receptors (Cat# PAHS064ZC-12, Qiagen, Germantown, MD), RNA extraction (RNeasy Pls Mini kit ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded into the Pig Inflammatory Cytokines & Receptors RT2 ProfilerTM PCR Array (Qiagen, Hilden, Germany). The qPCR reactions were performed using a Qiagen Rotor-Gene Q real-time cycler ...
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bioRxiv - Neuroscience 2020Quote: RNA from rat Schwann cell cultures or mouse nerve tissue was extracted using an RNeasy Micro Extraction Kit (Qiagen). RNA quality and concentration was determined after extraction using a nanodrop 2000 machine (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... TCRαβ+CD4-CD45RClow/- T cells from Il34+/+ and Il34-/- rats were extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and protocol of 3’ DGE RNA sequencing was performed as previously described (16) ...
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bioRxiv - Genomics 2021Quote: ... Then 37.5μl of beta-mercaptoethanol (0.375% final) and 10μl RNAse A (Qiagen® 100mg/mL) were added ...
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bioRxiv - Immunology 2020Quote: ... cDNA was added to the Human Toll-like receptor signaling pathway RT2 Profiler PCR array (Qiagen) and run on an iCycler MyiQ (Bio-Rad) ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Genomics 2019Quote: ... FSM sub-groups and 12 rats in the CS sub-group) using the RNeasy Plus Mini Kit (Qiagen, Hilden Germany). RNA extraction ...
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bioRxiv - Bioengineering 2020Quote: ... 100 ng equivalent of pooled total RNA (5 slices per animal; 7 rats) was used to synthesize cDNA using an RT First Strand Kit (Qiagen). Pre-validated primers targeting rat CXCR4 ...
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bioRxiv - Neuroscience 2021Quote: Total brain RNA from 21 days old Itm2bD/D and Itm2bww/ rats (2 male and 2 females per each genotype) was extracted with RNeasy RNA Isolation kit (Qiagen). Standard RNA-Seq procedures and data analysis was performed by Genewiz following proprietary methods (https://cdn2.hubspot.net/hubfs/3478602/NGS/RNA-Seq/GENEWIZ_RNA-Seq_Technical_Specifications_US.pdf) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: DNA and RNA were extracted from rat liver using the All Prep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany) using the manufacturer’s instructions for ‘Simultaneous Purification of Genomic DNA and Total RNA from Animal and Human Tissues’ with no alterations ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from 700,000 to 1.25 million RGCs from 4 independent rat litters per developmental stage was isolated using the Qiagen RNAeasy kit from Qiagen, which included a 15 minutes On-column DNAse step ...
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bioRxiv - Neuroscience 2023Quote: The extraction and purification of adult Sprague-Dawley rat RNA was performed using a RNeasy Mini Kit (Qiagen, Germantown, MD). cDNA was obtained using High-Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from mouse and rat embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen, Düsseldorf, Germany). cDNA constructs were inserted in TOPO II PCR-plasmids and using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: DNA was isolated from 1 mm3 mouse and naked mole-rat back skin biopsies and livers using a modified and combined version of the AllPrep DNA/RNA Micro Kit (Qiagen) and DNeasy Blood and Tissue Kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... DNA was extracted from 10 g of sediment using the PowerMax Soil DNA Isolation Kit (12988-10, QIAGEN) according to the manufacturer’s protocol with minor modifications for the step of homogenization and cell lysis i.e. ...
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bioRxiv - Molecular Biology 2022Quote: Rat epididymal fat and liver tissue samples were lysed in RNA extraction buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD) with stainless-steel disruption beads for 4 minutes at 30.0 Hz using the Qiagen TissueLyser (Germantown ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 ng of cDNA was amplified using Pyromark PCR kit (Qiagen) with final primer concentration of 0.4 uM ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mL of Buffer S3 (From QIAGEN Plasmid Plus Midi Kit) was added to the mixture ...