Labshake search
Citations for Qiagen :
451 - 500 of 10000+ citations for Rat Heme Oxygenase 1 HMOX1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... PCR amplifications were carried out in 50-μL total volumes containing 1 μL of template using a DNA HotStarTaq Master Mix kit (Qiagen). The cycling protocol was as follows ...
-
bioRxiv - Plant Biology 2021Quote: ... and sesame (cv. Kanto-1) using an RNeasy Plant Mini Kit™ according to the manufacturer’s instructions (Qiagen, https://www.qiagen.com). Reverse transcription of RNAs were carried out using PrimeScript II 1st strand cDNA Kit™ (Takara ...
-
bioRxiv - Cell Biology 2021Quote: ... The extracellular RNAs from 1 mL CPB patient plasma samples were isolated using the exoRNeasy Midi kit (Qiagen, Cat# 77044).
-
bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.2 μM gene specific primers pairs (hVDAC1, hVDAC2, hVDAC3, β-actin (Table 1) and 12.5 μl of master mix (QuantiFast SYBR Green PCR kit, Qiagen). Three independent experiments of quantitative real-time were performed in triplicate for each sample ...
-
bioRxiv - Microbiology 2021Quote: ... The digested DNA was separated on a 1% agarose gel and the ∼5 Kb genomic DNA band was harvested with a QIAquick Gel Extraction Kit (Qiagen). The DNA library was prepared according to the manual of the Ligation Sequencing Kit (Nanopore) ...
-
bioRxiv - Pathology 2022Quote: ... and 500 ng to 1 μg of DNA was treated with bisulfite using an EpiTect DNA bisulfite kit (QIAGEN, Germany) according to the manufacturer’s guidelines ...
-
bioRxiv - Immunology 2022Quote: ... and homogenized via sonication at 20 kHz for 1 min before RNA isolation was performed using a RNeasy Mini Kit (Qiagen). For qPCR ...
-
bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
-
bioRxiv - Genomics 2019Quote: ... 2D monolayers were lysed in RLT buffer supplemented with 1% 2-Mercaptoethanol before RNA purification using the RNeasy Mini Kit (QIAGEN) according to the manufacturer’s instructions and including the on-column DNase digestion step ...
-
bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). DNA was PCR amplified for 12 cycles with Illumina primers PE1.0 and PE2.0 and Phusion DNA polymerase (1 unit ...
-
bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 1×10^7 PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany). Following nucleic acid isolation ...
-
bioRxiv - Genomics 2019Quote: Cells aliquoted for RNA processing were spun down and resuspended in 3.2 mL of RLT (1% ß-Mercaptoethanol) from a Qiagen RNeasy Midi kit (Qiagen). Cells were homogenized by passing the lysate through an 18-gauge needle 10 times and were stored at −80°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Eight to ten hundred adherent DLD-1 cells were washed with PBS and lysed directly with RLT buffer from RNAeasy Mini Kit (Qiagen). Extracted RNA was analyzed for 260/280 and RIN value on Bioanalyzer 2100 (Agilent) ...
-
bioRxiv - Microbiology 2020Quote: ... and positive swabs at 3 days post infection (d.p.i.) with a Cq value < 30 (Table 1) using QIAamp viral RNA Mini kit (Qiagen, Hilden, Germany) according to the kit protocol ...
-
bioRxiv - Synthetic Biology 2020Quote: Cells were grown in triplicates on JMM 40 mM glycine and 40 mM pyruvate till mid-log phase and 1-2 mL of cultures were harvested and stabilized directly by RNA Protect Bacteria Kit (Qiagen). Cells were then lysed using lysozyme and beating with glass beads in a Retschmill (MM200 ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA was finally purified by reverse crosslinking at 65°C overnight followed by proteinase K (0.5 or 1 mg/ml, respectively) digestions and purifying with MinElute® PCR purification kit (Qiagen).
-
bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
-
bioRxiv - Microbiology 2020Quote: ... HIV-1 integration was examined in 293T cells and J-Lat cells by isolating genomic DNA from 1 × 106 cells using the DNeasy Tissue Kit (Qiagen). Alu-LTR sequences were amplified during first round PCR from 100 ng of total genomic DNA using the following primers ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
-
bioRxiv - Bioengineering 2021Quote: ... for 1 day prior to extraction of total RNA of the treated leaves with an RNeasy plant mini kit (QIAGEN). Two-step qPCR was performed to quantify GFP gene silencing using the total RNA extracted from leaves ...
-
bioRxiv - Genomics 2021Quote: ... The PCR products were size-selected on a 1% agarose gel and purified using the MinElute PCR Purification Kit (Qiagen). The pSTARR-seq_human vector (kindly provided by Alexander Stark ...
-
bioRxiv - Immunology 2021Quote: ... A total of 1-2 μg of RNA was used to synthesize the first single-strand cDNA using QuantiTect Reverse Transcription kit (Qiagen). For RT-PCR amplification ...
-
bioRxiv - Immunology 2020Quote: RNA was extracted from isolated neutrophils after the 1 and 4 hour EC challenges as well untreated controls using the miRNeasy Micro kit from Qiagen and libraries were generated using KAPA PolyA enrichment mRNA library prep ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were electrophoresed in a 1% agarose gel and the gel bands were isolated using QIA Quick PCR Purification Kit (Qiagen). Isolated DNA was cloned into pCR-TOPO plasmids using the TOPO TA cloning kit (ThermoFisher Scientific) ...
-
bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
-
bioRxiv - Cancer Biology 2019Quote: Genomic DNA was extracted from up to 1 mL whole blood or buffy coat using the Gentra Puregene Blood Kit (Qiagen) as per the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... The template was run on a 1% agarose gel and extracted using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). DNA template concentration was determined by a Qubit 3.0 Fluorometer (Life Technologies ...
-
bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
-
bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
-
bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
-
bioRxiv - Immunology 2020Quote: RNA was isolated from individual pools of 1×104 to 2×104 double-sorted SLAM cells using an RNEasy Micro kit (Qiagen). RNA was quantified and quality checked using an Agilent Bioanalyzer 2100 (Agilent) ...
-
bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
-
bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
-
bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
-
bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
-
bioRxiv - Cancer Biology 2020Quote: ... or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23) ...
-
bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto pools using the QIAsep 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation before sequencing on an Illumina MiSeq platform at the Duchossois Family Institute Microbiome Metagenomics Facility at the University of Chicago ...
-
bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified by ethanol precipitation and 1% agarose gel purified using the QIAquick Gel Extraction Kit (Qiagen). Single-round E ...
-
bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
-
bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...