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Citations for Qiagen :
1 - 50 of 10000+ citations for Rat Heat Shock Protein HSP 90 Beta HSP90AB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... heat-shock and buffer C1 of the DNAeasy powerlyzer Powersoil kit from Qiagen (previously known as the MoBio Powerlyzer PowerSoil DNA isolation kit from MoBio) ...
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bioRxiv - Microbiology 2022Quote: ... Gene expression of 84 heat shock genes in mock-infected or 229E-infected cells was analyzed using Human Heat Shock Proteins & Chaperones RT2 Profiler PCR array (Qiagen). Real-time PCR analyses were performed with specific primers ...
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bioRxiv - Plant Biology 2020Quote: Five-day old seedlings treated with heat-shock as described above were subjected RNA preparation using RNAeasy kit (Qiagen). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... coli following a standard heat shock protocol then isolated by miniprep (Cat. No. 27104, QIAprep Spin Miniprep Kit, Qiagen, Germantown, MD, USA) following the manufacturer’s instructions and eluted in molecular grade water to minimize the salt concentration for electroporation into N ...
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bioRxiv - Genetics 2021Quote: RNA was isolated from shock frozen liver samples using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: RNA was isolated from shock frozen liver samples using the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... HSP90AB1) and fold changes/regulation of gene expression were calculated using the 2^(−ΔΔCT) formula (GeneGlobe, QIAGEN). Differential expression (up and down regulation ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Genetics 2020Quote: ... with beta-actin as a reference gene (QuantiFast SYBR Green PCR Kit; Qiagen) on a Roche LightCycler 480 ...
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bioRxiv - Plant Biology 2023Quote: ... followed by heat inactivation and purification using a gel extraction kit (Qiagen). 50 ng of the DNA were incubated with different combinations of NHEJ proteins (25 nM AtKu70/80 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic DNA was extracted from 90 species (supplementary data) using the DNEasy kit (Qiagen USA) and was then sheared using a timed Fragmentase protocol and assessed with Bioanalyzer ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT (with 143mM beta-mercaptoethanol) and RNA purified using the RNeasy kit (Qiagen). 0.8-2μg of RNA were reverse transcribed using the High-Capacity cDNA reverse transcription kit and quantitative real-time PCR was carried out with the ViiA 7 Real-time PCR system (both Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted from 100 µl of heat-killed bacterial culture using the MagAttract kit (Qiagen). Sequencing libraries were constructed using 1ng of input DNA with a modified Nextera kit (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... + 5% beta-mercaptoethanol using a bead mill (Qiagen). Samples were heated at 95° for 5 minutes to denature proteins ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted in RLT buffer supplemented with beta-mercaptoethanol and processed with the RNeasy Micro Kit (QIAGEN) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in RLT buffer with Beta-mercaptoethanol and RNA was extracted using Qiagen RNEasy kit (Qiagen; #74134). Extracted RNA was then converted to cDNA using the one-step qSCRIPT cDNA solution ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized in Buffer RLT+ beta-mercaptoethanol (Qiagen). Tissue homogenate was then centrifuged through a QIAshredder homogenizer (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... and beta-mercaptoethanol using the TissueLyser II (Qiagen, Australia). DNA was extracted using the AllPrep DNA/RNA Mini Kit following the manufacturer guidelines (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... 80–90% depletion of OGT/OGA mRNAs was verified by RT-PCR using QuantiTect Reverse Transcription Kit (205311, Qiagen), QuantiTect SYBR Green PCR Kit (204143 ...
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bioRxiv - Microbiology 2020Quote: Heat inactivated samples were extracted using the Qiacube HT and the Cador pathogen extraction kit (both from Qiagen). Viral RNA was quantified by RT-qPCR (qRT-PCR EXPRESS One-Step Superscript™ ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Cell Biology 2021Quote: AllPrep DNA/RNA/Protein Mini Kits (Qiagen) were used to extract total proteins from UVA-exposed and non-exposed HTEpC from all experiments ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNAs were isolated from cerebral cortices (P15 rats) using Mini RNeasy kit (Qiagen) then converted to cDNA using 1 µg RNA and a QuantiTect Reverse Transcription kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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bioRxiv - Genetics 2022Quote: ... and sex-matched controls were grown to 80-90% confluency in T75 flasks before total RNA extraction via RNeasy Plus Mini Kit (Qiagen, 74136). Whole blood samples were collected and purified using MagMAX for Stabilized Blood Tubes RNA Isolation Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen). RNA was harvested from three independent cultures as biological replicates per experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... were heat killed in a 95°C water bath for 20 min to produce thermolysates before DNA extraction (MagAttract kit, Qiagen) and whole-genome sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Cancer Biology 2020Quote: The All Prep DNA/RNA/Protein kit (QIAGEN) was used to total RNA from cell lines ...
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bioRxiv - Microbiology 2022Quote: ... Reference plasmids were serially diluted in either PBS buffer or heat-inactivated normal human serum (NHS) and re-extracted using the QIAamp® DNA Blood Mini Kit (Qiagen) per recommended protocol for serum ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from cultured mycobacteria by heat lysis and purified on QIAamp DNA Mini Kit spin columns (Qiagen Düsseldorf, Germany) in the isolating laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was extracted 48 hours post transfection with All Prep RNA/Protein Kit (Qiagen, USA). Protein concentrations were determined by Lowry assay (Bio-Rad ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... protein extraction was performed using the Allprep RNA/Protein Kit (80404 Qiagen Inc., Hilden, Germany). Proteins (10–20 µg ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was isolated from powdered tissues using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein pellets were lysed in HES-SDS buffer (20 mM HEPES [pH 7.4] ...
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bioRxiv - Cell Biology 2023Quote: Cells were collected for total protein using Qproteome Mammalian Protein Prep Kit (Cat. 37901, Qiagen). Lysates were quantified for protein expression utilizing the WES system (ProteinSimple ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and WPMY1 parental cell lines were seeded in 6-well plates and grown for 24 hours to a confluency of 70% to 90% before RNA was extracted using an RNeasy RNA extraction kit (Qiagen, Venlo, the Netherlands). Samples were prepared in triplicate ...
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bioRxiv - Neuroscience 2023Quote: ... 10% of the cells were used for passaging and DNA was extracted on the remaining 90% of the cells using a Qiagen DNA extraction kit (Qiagen; Cat. No. 69504). Clones were screened by PCR (DLK KO primers ...