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Citations for Qiagen :
351 - 400 of 1566 citations for Rat CPS1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... coli colonies were collected with scrapers and the library plasmids were purified using Plasmid Maxi Kit (Qiagen). Lentiviral libraries were prepared as described above in complete DMEM and harvested using PEG-it Virus Precipitation Solution (System Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... a control plasmid containing the FKBP12F36V insert was purified from a bacterial clone (QIAGEN Plasmid Maxi kit). Six serial dilutions of the plasmid were generated ...
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bioRxiv - Biochemistry 2022Quote: ... bacteria were cultured and plasmids were harvested according to the QIAGEN Plasmid Plus Maxi Kit protocol (QIAGEN). For mammalian expression and purification of KWOCAs and other secreted proteins ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria were cultured in lysogeny broth overnight and plasmid was harvested with Plasmid Plus Midi Kit (QIAGEN). This intermediate library and pJR98 were digested using BsmBI and the insert from pJR98 was ligated into the intermediate library to insert constant region CR3* for the first sgRNA and the hU6 promoter for the second sgRNA ...
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bioRxiv - Genomics 2023Quote: ... the epegRNA lentiviral plasmid libraries were extracted using a Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143). Extracted plasmid DNA from the four libraries was pooled to generate a pool with 3,825 epegRNAs and used for lentivirus generation.
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA was extracted directly from the pooled colonies using a QIAGEN Plasmid Maxi Kit (QIAGEN, #12162).
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bioRxiv - Developmental Biology 2023Quote: ... Colonies were scraped and plasmid DNA (pDNA) of the sgRNA library was prepared (HiSpeed Plasmid Maxi, Qiagen). Deep-sequencing confirmed that all sgRNAs were well represented in the EpiChromo library ...
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bioRxiv - Neuroscience 2023Quote: ... All the plasmids for AAV vector preparations were made using endotoxin free plasmid maxiprep kit (#12663, Qiagen). PEI mediated co-transfection of pAAV plasmid-containing the transgene ...
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bioRxiv - Genetics 2023Quote: ... Plasmids were amplified in competent E.coli and purified by using an Endofree plasmid max kit (#12362, Qiagen). Plasmids DNA were sent for Sanger sequencing to confirm the introduced SIRT5 variants (BaseClear ...
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bioRxiv - Genomics 2023Quote: ... The transfected plasmid DNA was extracted from the cells using QIAGEN Plasmid Plus Midi kit (QIAGEN 12943). The sequencing ready plasmid library was generated by PCR with the extracted plasmid ...
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bioRxiv - Immunology 2023Quote: ... Plasmids with the cloning of synthesized antibody genes were prepared using the Megaprep plasmid plus kit (Qiagen) for transient transfection ...
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bioRxiv - Cell Biology 2019Quote: ... The siRNA targeting rat Psmc1 included Rn_RGD:621097_1 FlexiTube siRNA (Qiagen, Cat# SI02002427) and Rn_RGD:621097_2 FlexiTube siRNA (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from cultured rat embryonic striatal neurons following stimulation (RNeasy, Qiagen) and submitted to the Genomic scorelab at the Heflin Center for Genomic Sciences at the University of Alabama at Birmingham for library preparation as previously described17,47 ...
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bioRxiv - Biophysics 2021Quote: ... coli culture were harvested next morning to extract the plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transformed into XL1-Blue competent cells and plasmid DNA extracted using QIAprep Spin Miniprep Kit (Qiagen). The BUB1 K821R and BUB1C D946N alleles were generated by single-base substitution using site-directed mutagenesis (Strategene ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids and PCR products were purified with QIAprep plasmid Miniprep kit and QiaQuick PCR purification kit respectively (Qiagen). Plasmid Sanger sequencing was performed by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
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bioRxiv - Microbiology 2019Quote: ... Coli Top10 strain was transformed with plasmids followed by their amplification and isolation using Plasmid Midi Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... plasmids were isolated from 200 mL of these cultures using Qiagen Plasmid Plus Purification kits (Qiagen #12963, #12965). The remaining 200 mL of the overnight cultures were spun down ...
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bioRxiv - Cancer Biology 2022Quote: ... Colonies were harvested using a cell scraper and plasmids were purified using the HiSpeed Plasmid Maxi Kit (QIAGEN). We confirmed homogeneous library representation by next-generation sequencing (Figure S2C).
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids used for in ovo electroporation were purified using the 16 Qiagen Plasmid Plus Midi Kit (Qiagen). The total concentration of DNA ranged for each set of experiments between 0.5–1μg/μl ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were purified from positive clones with a QIAprep Spin Miniprep plasmid kit as instructed (Qiagen; Germantown, MD), and verified by Sanger automated sequencing (ACGT ...
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bioRxiv - Microbiology 2020Quote: ... Plasmid DNA from the Dub-seq library samples was extracted either individually using the Plasmid miniprep kit (Qiagen) or in 96-well format with a QIAprep 96 Turbo miniprep kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: The recombinant plasmid DNA derived from positive clones was isolated by using the QIAGEN plasmid mini kit (QIAGEN) and digested by PmlI (vector PFLD ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were prepared using a Qiagen Plasmid Plus Midi Kit and a QIAvac vacuum manifold (Qiagen, Cat#: 12145).
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bioRxiv - Genomics 2021Quote: ... The bacterial cells were harvested by centrifugation and plasmid DNA was isolated using EndoFree Plasmid Giga kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... The bacterial cells were harvested by centrifugation and plasmid DNA was isolated using EndoFree Plasmid Giga kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... Each plasmid was first propagated in DH5α cells and later isolated utilizing the plasmid isolation kit from Qiagen. SH-SY5Y cells were transiently overexpressed with respective plasmids via standard protocol provided for Lipofectamine3000 mediated transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were grown using ByLss cell transformation procedures and extracted using the EndoFree Plasmid Maxi Kit (Qiagen, 12362). Final nucleic acid concentrations were determined using a NanoDrop (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... Colonies were scraped and plasmid DNA was prepared using the HiSpeed Plasmid Maxi kit (Qiagen, catalog no. 12662) with the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was purified from 130 mL of liquid culture using a HiSpeed Plasmid Maxi Kit (Qiagen 12663).
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bioRxiv - Biophysics 2022Quote: ... coli culture were harvested next morning to extract the plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN).
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bioRxiv - Genomics 2023Quote: ... coli culture were harvested next morning to extract the plasmid library using the QIAfilter Plasmid Midi Kit (QIAGEN). Finally ...
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmid DNA extracted via miniprep (Qiagen). The resulting plasmid pool was used for downstream analysis via junction PCR and long-read sequencing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... or a HiSpeed Plasmid Maxi Kit (Qiagen). To examine the functionality of select putative AHR or GR binding sites ...
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bioRxiv - Neuroscience 2021Quote: ... and purified with Plasmid Plus Kit (QIAGEN). The lentiviruses were packaged in Lenti-X 293T cell line (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by plasmid miniprep (Qiagen MiniPrep Kit). Correct assembly of the HC and LC sequences into expression vectors were confirmed by sanger sequencing of the purified plasmids (Eurofins Genomics).
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Neutralizing PD-L1 and PD-L2 Enhances the Efficacy of Immune Checkpoint Inhibitors in Ovarian CancerbioRxiv - Cancer Biology 2020Quote: ... and isolated using plasmid miniprep kit (Qiagen). Sequencing was performed by Sequetech Corp.
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bioRxiv - Immunology 2019Quote: ... and used EndoFree plasmid maxi kit (QIAGEN) to isolate DNA for the booster immunizations ...
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bioRxiv - Neuroscience 2020Quote: ... and EndoFree Plasmid Maxi Kit (Qiagen, 12362), and verified them via Sanger sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmid maxi prep kit was from QIAGEN. HaloTag® Alexa Fluor® 488 Ligand was from Promega ...
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bioRxiv - Neuroscience 2020Quote: ... The cloned plasmids were purified by Qiagen purification kit and were then injected into one-cell-stage zebrafish oocytes at 40 ng/μl together with 30 ng/μl Tol2 transposase mRNA ...
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bioRxiv - Biophysics 2020Quote: ... and after plasmid amplification (QIAGEN, Valencia, CA). All constructs were in pcDNA3.1(+ ...
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bioRxiv - Genomics 2022Quote: ... Plasmid DNA was extracted (Qiagen Miniprep Kit) and analyzed by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... We transformed purified plasmids (QIAgen mini prep) into B ...
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bioRxiv - Cell Biology 2022Quote: ... and EndoFree Plasmid Maxi Kit (#12362, Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... All batches of purified plasmids (Qiagen, 12165) were confirmed by DNA sequencing.
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bioRxiv - Genomics 2019Quote: ... and plasmid isolation (Qiagen Mini-Prep Kit).
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bioRxiv - Synthetic Biology 2020Quote: ... and Plasmid Plus Maxi Kit (Qiagen, 12965) respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... prepared using the Plasmid Midi Kit (Qiagen), and transfected into 293FT cells using the Virapower packaging mix (Invitrogen ...