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Citations for Qiagen :
1 - 50 of 100 citations for Radio Flow Detectors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Amplification was performed using a Rotor-Gene Q real-time fluorescence detector thermocycler (Qiagen) programmed as follows ...
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR was performed using a DNA Engine Opticon Continuous Fluorescence Detector (MJ Research) and a Quantitect SYBR Green PCR kit (Qiagen). We used the following primer pairs ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Synthetic Biology 2019Quote: ... was added to a 15 mL polypropylene gravity flow column (Qiagen), storage buffer was allowed to flow through by gravity ...
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bioRxiv - Biochemistry 2020Quote: ... The flow through from the second Ni-NTA agarose resin (Qiagen) incubation was spun at 20,000 x g for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Cells sorted by flow cytometry were lysed in RLT buffer (Qiagen). RNA was purified using RNeasy mini kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and stored up to 2mL of the flow-through in RNAprotect (Qiagen). Parasite density was estimated from light microscopy ...
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bioRxiv - Genomics 2020Quote: ... loaded onto a gravity flow column packed with Ni-NTA agarose (Qiagen), and rotated for 2h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Equally amounts of Strep-Tactin super flow plus beads (15 μL, Qiagen) were saturated with DOCKDHR2 proteins (40 μg) ...
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bioRxiv - Microbiology 2021Quote: Proteins were purified by gravity flow using Ni-NTA agarose (Qiagen, 30210) in 2 mL chromatography columns (Bio Rad ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were purified using gravity flow Ni-NTA (Qiagen, Monheim, Germany) affinity chromatography ...
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bioRxiv - Physiology 2023Quote: ... Agarose beads were collected in 1 ml propylene gravity flow columns (Qiagen) and washed with 10 ml Resuspension buffer ...
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bioRxiv - Biochemistry 2023Quote: ... which was then loaded to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB3_2 ...
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bioRxiv - Systems Biology 2021Quote: ... Data graphing and visualization was performed using Partek Flow (Partek) and IPA (QIAGEN) graphing and visualization modules ...
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bioRxiv - Neuroscience 2020Quote: ... Data graphing and visualization was performed using Partek Flow (Partek) and IPA (QIAGEN) graphing and visualization modules ...
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bioRxiv - Biophysics 2021Quote: ... DNA plasmids were purified using anion-exchange gravity flow chromatography (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2020Quote: ... FeCl3-NTA beads were prepared from Ni-NTA super flow slurry beads (QIAGEN) by first stripping the beads by incubating with 100 mM EDTA in a vacuum manifold three times ...
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bioRxiv - Synthetic Biology 2022Quote: ... The centrifuge flow-through was discarded and 400 µL of buffer PE (Qiagen) was added to the column ...
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bioRxiv - Immunology 2020Quote: RNA from flow-cytometry sorted BMDCs was isolated using the RNeasy kit (Qiagen), per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was applied to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB4_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted from the flow-through using the RNeasy Plus Mini Kit (QIAGEN). RNA was aliquoted into at least three tubes to minimize freeze-thaw cycles while allowing for QC analysis using a Bioanalyzer ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... screened by flow cytometry and their plasmidic DNA was isolated using miniprep kit (Qiagen), transformed in DH10B and re-isolated for sequencing.
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bioRxiv - Biophysics 2020Quote: ... Cleaved samples were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN) and flow-through was collected ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted from the flow-through using the RNeasy Mini Kit (Qiagen, 74104) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMCs were used immediately for flow cytometry or stored in RNA Protect (Qiagen) according to the manufacturer’s instructions for RNA isolation ...
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bioRxiv - Microbiology 2023Quote: ... and affinity-purified in a gravity flow column using Ni2+-NTA-agarose beads (Qiagen). After binding of the His-tagged proteins to the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... ∼500 flow sorted cells were resuspended in 2.5 µl of RLT plus buffer (Qiagen). Polyadenylated RNA was captured with oligo-dT conjugated beads and processed using Smart-seq2 protocol(66) ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were loaded onto a gravity-flow column containing Ni–NTA resin (Qiagen) equilibrated in lysis buffer (without PMSF) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocytes purified by flow cytometry and cultured cells were collected in lysis buffer (Qiagen) containing 1% β-mercaptoethanol for optimal template preservation ...
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bioRxiv - Cell Biology 2023Quote: ... flow sorted into 96 well plates containing 2.5ul of RLT plus lysis buffer (Qiagen), collecting only viable singlets ...
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bioRxiv - Genomics 2024Quote: ... DNA was isolated using the gravity flow Genomic Tip kit (Qiagen, cat. no. 10223) and by the phenol-chloroform method 17 ...
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bioRxiv - Genomics 2022Quote: ... Flow-through was discarded from the collection tube and 500 µl of buffer RPE (Qiagen) were added to the column and centrifuged for 30 s ...
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bioRxiv - Systems Biology 2019Quote: ... Unbound (unbiotinylated) RNA from the flow-through was recovered using the RNeasy MinElute kit (Qiagen) and reconstituted in 25 μL of RNase-free water ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from flow cytometry purified cells using RNeasy Mini kit (Qiagen, Hilden, Germany) with on-column DNase digest ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was applied to a gravity flow column packed with Ni-NTA resin (Qiagen), followed by 20ml wash buffer (25mM Tris-HCl pH=8 ...
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bioRxiv - Synthetic Biology 2023Quote: ... All proteins were purified by gravity flow Ni2+ -NTA affinity chromatography (Qiagen, Cat. -No. 30230), except TEV-construct which was purified by gravity flow Strep-Tactin®XT Superflow® high-capacity resin (IBA Cat ...
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bioRxiv - Immunology 2021Quote: ... the supernatant/Sepharose bead slurry was passed through a 5 ml polypropylene gravity flow column (Qiagen). The column was washed with 1 column volume of PBS before being eluted with 9 ml of Elution Buffer (0.1M Glycine/HCl buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... clones were screened by flow cytometry and their DNA was isolated using a miniprep kit (Qiagen), transformed into DH10B and re-isolated for sequencing.
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bioRxiv - Cancer Biology 2020Quote: Total RNA from flow cytometry enriched populations was extracted using RNeasy Mini Kit (Qiagen, Venlo, Netherlands) and reverse transcribed using the QuantiTect Reverse Transcription kit (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto the affinity matrix equilibrated with buffer A by gravity flow (Qiagen, Ni-NTA agarose slurry ...
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bioRxiv - Molecular Biology 2021Quote: ... The flow-through was collected and applied to 0.4 mL volume Ni-NTA Agarose resin (Qiagen) to remove the 7×His-TEV protease ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen, Hilden, Germany). The eluted protein was subjected to MonoQ 10/100 GL (GE Healthcare Lifesciences ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted from flow-sorted CD3+ cells using the RNEasyPlus Micro Kit (Qiagen, 74034), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cells were washed twice with 40 µL/well flow buffer and stained with streptavidin-PE (Qiagen) for 30 min at 4°C ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
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bioRxiv - Systems Biology 2019Quote: ... 390 µl Ethanol (100%) was added to the flow through and loaded on a MinElute column (Qiagen). Columns were washed following the manufacturer’s protocol and RNAs were eluted in 15 µl RNase-free H2O.
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from flow cytometry-sorted cells using the RNeasy Mini kit (Qiagen, Germantown, MD). Two hundred nanograms of total RNA were reverse-transcribed into cDNA using random hexamers with the Taqman Reverse transcription kit (Invitrogen) ...