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Citations for Qiagen :
1 - 50 of 385 citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and the GFP expression vector pEGFP-N1 were linearized with DraIII and treated with alkaline phosphatase before purifying with a PCR purification kit (Qiagen). Then 500 ng each of circular or linearized pGEM-Dik-LT/O and pEGFP-N1 were co-transfected into 293 cells with Lipofectamine 2000 reagent according to the manufacturer’s instruction and the medium was replaced with fresh DMEM four hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... or 7-recrudescent populations as well as PruQ tachyzoites and alkaline-induced PruQ bradyzoites (pH8.2 media for 72 h) were purified using the RNeasay kit (Qiagen) according to manufacturer protocols ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2022Quote: ... Bacmid DNA was isolated via alkaline lysis (Qiagen buffers P1, P2 and P3) and isopropanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... BAC DNA was purified from 200ml bacterial cultures by alkaline lysis (Qiagen buffers), and circular DNA was separated by CsCl ultracentrifugation ...
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bioRxiv - Genomics 2019Quote: ... the cells were incubated in an alkaline solution (buffer DLB and DTT, Qiagen, Germany) for 5 min at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... we midi-prepped these cultures via alkaline lysis (Qiagen Plasmid Plus Midi-Prep Kit). This first cloning step enabled generation of intermediate products ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were subject to alkaline lysis by adding 250ul of P2 lysis buffer (Qiagen, 19052) for 5 mins and then neutralized by addition of Qiagen N3 neutralization buffer (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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Differences in orthodenticle expression promote ommatidial size variation between Drosophila speciesbioRxiv - Evolutionary Biology 2021Quote: ... 96 h AEL and 120 h AEL and stored in RNALater (Qiagen, Venlo, Netherlands). Three biological replicates for each sample were generated ...
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bioRxiv - Physiology 2021Quote: 500ng mouse or 300ng sheep RNA was reverse transcribed using QuantiTect Reverse transcription kit (Qiagen, Manchester, UK) and gDNA wipe-out (to remove genomic DNA) ...
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bioRxiv - Microbiology 2023Quote: ... RNA was removed by alkaline hydrolysis and cDNA was purified with PCR cleanup columns (Qiagen 28115). Next ...
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit ...
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bioRxiv - Microbiology 2021Quote: ... RNA was removed by alkaline hydrolysis and cDNA was purified with PCR clean-up columns (Qiagen; #28115). Next ...
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bioRxiv - Neuroscience 2020Quote: ... 0.4 U/μL RNase H (Qiagen), and 0.5 U/μL Ampligase (Epicentre ...
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bioRxiv - Neuroscience 2020Quote: ... 0.4 U/μL RNase H (Qiagen), and 0.5 U/μL Ampligase (Epicentre ...
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bioRxiv - Neuroscience 2020Quote: ... 0.4 U/μL RNase H (Qiagen), 0.001 U/μl Phusion DNA polymerase (NEB) ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... B220 (RA3-6B2) and Ly-6C/G (RB6-8C5) and anti-rat IgG-coupled magnetic beads (Qiagen) as previously described [34] ...
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bioRxiv - Microbiology 2021Quote: ... lysed using alkaline lysis in buffer P2 and neutralized with buffer N3 (All reagents from QIAprep kits, QIAGEN). Following addition of buffer N3 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Molecular Biology 2022Quote: ... Isolated colonies were inoculated into 5 mL of LB with appropriate antibiotics and plasmids were purified using the alkaline lysis protocol and Qiaquick miniprep kit (Qiagen). Plasmids were verified by sequencing (GATC ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from 2 h and 10 h RPMI-grown and macrophage-internalized Cg cells using the RNeasy kit (Qiagen), followed by DNase I digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... 0.1 U/μL RNase H (QIAGEN, Düsseldorf, Germany)] ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 h using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Isolates were cultured overnight on sheep blood agar and plasmid DNA was extracted using Qiagen Plasmid Midi kit (Qiagen Inc, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... morulae (48 h post-IVF) and blastocysts (68 h post-IVF) were collected in 5uL of 1x TCL lysis buffer (Qiagen #1031586) containing 1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... phosphatase inhibitors 2 and 3) and then passing through Qiashredder columns (79656, Qiagen). Tissue samples were weighted ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from both initial mixtures (0 h incubation) and end point co-cultures (72 h incubation) using the DNeasy Power Biofilm extraction kit (DNeasy PowerBiofilm, Qiagen, Mississauga, ON) as described previously [5] ...
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bioRxiv - Systems Biology 2021Quote: ... and 200 L of Buffer AL (QIAGEN) were added to each sample before mixing with a pipette ...
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit, Qiagen, Inc., Germany).
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bioRxiv - Microbiology 2022Quote: ... 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8 ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated after 48 h with QIazol (Qiagen) and the Direct-zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 24 h cells were transfected with Lipofectamine 3000 (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... trigynum (Sample size: L. tenue thrum=6, pin=4, L. trigynum homostyle=5) using the RNeasy Plant Mini Kit (QIAGEN, Germany). Libraries were sequenced on an Illumina NovaSeq S1 Sequencing System to produce paired-end 150bp read length reads ...
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bioRxiv - Cell Biology 2019Quote: ... then mixed with BioMag goat-rat IgG beads (Qiagen) and Ter119+ cells were depleted using a Dynal magnet (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... and 72 h after induction using RNeasy Plant Mini Kit (Qiagen). RNA-seq analysis was carried out by Hokkaido System Science Co. ...
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bioRxiv - Microbiology 2019Quote: ... and 66 h post-infection using QIAmp DNA micro kit (QIAGEN). Viral DNA forms of wild-type and mutant HIV-1 were amplified using real-time PCR (Butler et al. ...
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bioRxiv - Microbiology 2022Quote: ... Pellicle biofilms (96 h) were homogenized with TissueLyser LT (QIAGEN, Germany) at 50 kHz for 10 mins ...
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bioRxiv - Plant Biology 2022Quote: ... 6 or 24 h using an RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Total RNA was harvested at 96 h after treatment (RNeasy, Qiagen). MCL1 and PPIB (housekeeping ...
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bioRxiv - Immunology 2022Quote: ... T6BP (L-016892-00-0020 Dharmacon or SI02781296, Qiagen), CANX (SI02663367 and SI02757300 ...
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bioRxiv - Systems Biology 2022Quote: ... FABP (horizon L-042923-01-0005) and HIF1α (Qiagen SI00193032 ...
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bioRxiv - Microbiology 2020Quote: ... 0.01% L-tryptophan) using the RNeasy Mini kit (Qiagen). Ten μg of total RNA was hybridized to 150 nM 32P 5’-end-labeled DNA oligonucleotide complementary to nucleotides +94 to +109 (relative to yfmHG transcription ...
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bioRxiv - Genetics 2022Quote: ... Quantified libraries (GeneRead DNA Library L Core Kit; QIAGEN) were sequenced using an Ion Torrent S5 XL instrument with Ion 530 chips and respective reagents (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... protease and phosphatase inhibitors) or RLT buffer supplemented with “- mercaptoethanol (RNeasy mini kit, Qiagen, Hilden, Germany) for western blot analysis and total RNA extraction ...