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Citations for Qiagen :
151 - 200 of 10000+ citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... or HiPerFect Transfection Reagent (QIAGEN).
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bioRxiv - Cell Biology 2023Quote: ... using Effectene Transfection Reagent (Qiagen). After 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... in HiPerFect transfection reagent (Qiagen) using a fast-forward transfection protocol then further siRNA is added 24 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... Effectene transfection reagent from Qiagen; QuickExtract DNA Extraction solution from Lucigen ...
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bioRxiv - Cell Biology 2023Quote: ... using Effectene Transfection Reagent (Qiagen). The fraction of GFP positive cells was scored after 48 h.
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bioRxiv - Developmental Biology 2023Quote: ... The Effectene transfection reagent (Qiagen) was used for cell transfection according to suppliers’ guidelines ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... HotStarTaq plus PCR reagents (Qiagen) were used for PCR amplification ...
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bioRxiv - Genetics 2024Quote: ... Reagent DX (Qiagen, Germantown, MD) was added to the lysis buffer at 0.5% v/v to prevent foaming during homogenization.
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bioRxiv - Microbiology 2024Quote: ... using HiPerFect transfection reagent (Qiagen). Six hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... using Effectene transfection reagent (Qiagen). Virus was collected starting 24 hours after transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were homogenized in QIAzol Lysis Reagent and subjected to RNA isolation using miRNeasy Micro Kit (Qiagen, Cat. No. 217084). Isolated RNA was then purified using RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Physiology 2021Quote: ... 0.5 to 3 million cells were re-suspended in 500 uL TRIzol Reagent and total RNA was extracted using the miRNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... while extraction from tissues was carried out using QIAzol Lysis Reagent and purified using RNeasy Plus Universal Mini Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were spun at 3500 x g for 15 min and RNA was extracted from the bacterial pellet with QIAzol lysis reagent and the miRNeasy Mini Kit (Qiagen). rRNA was removed with the Bacterial RiboMinus Transcriptome Isolation Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted with TRIzol reagent according to the manufacturer’s protocol and cleaned using a kit (QIAGEN, Hilden, Germany). RNA quality was assessed using bioanalyzer ...
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bioRxiv - Genomics 2022Quote: ... Total genomic DNA was extracted from each sample with commercial reagents and following manufacturer guidelines (Qiagen, DNAEasy Blood&Tissue kit). Details on origin ...
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bioRxiv - Genetics 2019Quote: The pyrosequencing genotyping was performed using single-stranded PCR products obtained using the PyroMark Q24 Vacuum Prep Workstation then used in pyrosequencing reactions performed using the PyroMark Gold Q96 reagent kit (Qiagen). The sequencing primer and dispensation order are described in Table 1.
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bioRxiv - Developmental Biology 2019Quote: Total RNA of 2 – 4 dpf zebrafish embryos was extracted using Trizol reagent and purified using the RNeasy Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: RNA was isolated using TRI Reagent® and further purified using the RNeasy Mini Kit (Qiagen, UK, Catalogue No. 74106) which included a DNase digestion step ...
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bioRxiv - Physiology 2020Quote: Total RNA from brown and white adipose tissues was isolated by Qiazol lysis reagent and using the protocol of the RNeasy Kit (Qiagen). Complementary DNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... OR) and total RNA was isolated using QIAzol Lysis Reagent and the RNeasy Lipid Tissue Mini Kit (Qiagen, Valencia, CA). The concentration and purity were determined using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... OR) and total RNA was isolated using QIAzol Lysis Reagent and the RNeasy Lipid Tissue Mini Kit (Qiagen, Valencia, CA). The concentration and purity were determined using NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: Homogenization of human carotid artery plaque tissue was performed in 700 µL Qiazol lysis reagent and total RNA was isolated using the miRNeasy Mini Kit (Qiagen) according to the manufactureŕs instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells seeded into 100 mm dishes were transfected at a confluence of 60 % with 2 µg of cDNA encoding PTH1RFRET with Lipofectamine 3000 Transfection Reagent Kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... and purified DNA was obtained using the DNA Blood 200µl protocol and the QIAsymphony DNA Mini kit reagent cartridge (Qiagen, 931236). Quantitative PCR was performed on the plasma derived DNA using the 7900HT Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: ... aqueous phase from the Trizol reagent and proceeded with RNA mini-prep using the RNeasy Micro Kit (Qiagen Cat. 74004). We next took eluted RNA and proceeded with reverse transcription to create cDNA libraries for RT PCR ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was subsequently fragmented using the Ambion RNA fragmentation reagent and cleaned up with the RNeasy MinElute Cleanup kit (Qiagen). RNA was eluted from the column with 9μl of nuclease-free water.
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bioRxiv - Immunology 2022Quote: ... 50,000 cells were lysed directly into 700 ul of QIAzol reagent and RNA was isolated using the miRNeasy Micro kit (Qiagen) with on-column DNase digestion ...
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bioRxiv - Systems Biology 2022Quote: 2 × 109 cells pretreated with TRIzol reagent were used for preparing RNA reference materials using RNeasy Maxi kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: 40-60 fish embryos or larvae were placed into trizol reagent and total RNA was extracted using the RNeasy Mini Kit (Qiagen). 1 μg of total RNA was treated with RQ1 Rnase-free DNase (Promega ...
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bioRxiv - Microbiology 2022Quote: Samples for bulk RNA sequencing were lysed in Trizol Reagent and total RNA was extracted using the miRNeasy mini kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Cortical regions from ischemic GFAP-IGF-IR KO mice and littermates were dissected and total RNA isolated using Trizol Reagent and RNeasy mini kit (Qiagen). RNA was recovered in RNase free water (Ambion) ...
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bioRxiv - Pathology 2022Quote: Total RNA was harvested from cells and tissues using Qiazol Lysis Reagent and isolated by following the protocol described in the RNeasy Lipid Tissue Mini Kit (Qiagen). Concentration and quality of RNA was determined using a Nanodrop (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed on the second sample of the beads using Trizol reagent and the miRNeasy mini kit (QIAGEN). An RNase-free DNase (QIAGEN ...
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bioRxiv - Physiology 2023Quote: Total RNA from tissues was isolated with the Qiazol lysis reagent and using the protocol of the RNeasy Kit (Qiagen). Complementary DNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: The hippocampus tissue of 44 weeks old mice was collected and lysed in RNeasy Lipid Tissue mini kit reagent (QIAGEN). RNA was extracted according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The hippocampus tissue of 44 weeks old male mice was collected and lysed in RNeasy Lipid Tissue mini kit reagent (QIAGEN). RNA was extracted according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was then used for pyrosequencing following the standard protocol of the PyroMark Gold Q96 Reagents kit (Qiagen). A sequencing cartridge was prepared with all four nucleotides ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was extracted from mixed stage growing populations using reagents from the Puregene Core Kit A (QIAGEN, Valencia, CA) and quantified using Qubit 3.0 fluorometer (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... The resulting lysate was then made up to 3 mL with QIAzol Lysis Reagent and mixed thoroughly before 1 mL lysate aliquots were processed using RNeasy Lipid Tissue Kit 74804 (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and the p5′UTR-ATF4-FLuc (WT ATF4-FLuc) or p5′UTR-ATF4 A235G-FLuc (mut ATF4-FLuc) plasmids using a Transfection reagent kit (Qiagen) as we previously described10 ...
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bioRxiv - Immunology 2022Quote: ... siRNA-transfection reagent complexes were generated using 2 μL HiPerfect transfection reagent (Qiagen, Germantown, MD) and 25-50 nM siRNA (non-specific control siRNA (sc-37007 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from cultured pHCEnC was isolated in TRI Reagent and purified with the RNeasy Clean-Up Kit (Qiagen, Hilden, Germany). Total RNA from cultured MCEnC was isolated and purified using the Qiagen RNeasy Plus Mini Kit.
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bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
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bioRxiv - Neuroscience 2019Quote: ... 0.2 ng of the pCMV-TPBG-sport6 vector was transfected using the Effectene transfection reagent kit (Qiagen; Venlo, Netherlands; Cat# 301525), and expression was assessed by immunostaining approximately 24 hours after transfection.
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bioRxiv - Cancer Biology 2020Quote: Total RNAs were isolated from the A549 cells transfected with PD-L1-lnc overexpressed vector or control vector by TRIzol reagent and purified by RNeasy Mini Kit (Qiagen, USA). RNA samples were performed to Microarray analysis by Agilent SurePrint G3 human gene expression Microarray 8X60K (Agilent Technologies) ...
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bioRxiv - Genomics 2021Quote: ... was transferred using Pasteur blob to a clean Falcon tube containing 4.5 mL HEMAgene®•BUFFY COAT DNA stabilizing reagent (Oragene) and the DNA was extracted using QIAamp DNA Blood Mini Kit (Qiagen). The Coriell-NA12878 DNA sample was purchased from Coriell Cell Repositories ...
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bioRxiv - Neuroscience 2021Quote: ... Total cellular RNA was extracted from amygdala and NAc punches using Qiazol reagent and the RNAeasy Midi kit (QIAGEN, Valencia, CA). Total RNA was reverse transcribed into cDNA using VILO master mix (Invitrogen ...