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Citations for Qiagen :
1 - 50 of 137 citations for REST NRSF Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and Rest 2009 software (Qiagen). All experiments were performed with triplicates as described before (7 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Rest 2009 software (Qiagen). The relative expression level (2-ΔCT ...
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bioRxiv - Neuroscience 2020Quote: ... Promega) and dominant-negative REST (DN-REST)70 or GFP expression plasmids using Polyfect (Qiagen). Luciferase activities were measured 24 hours later ...
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bioRxiv - Cell Biology 2021Quote: ... and data were analyzed by the REST Software (Qiagen). Relative expression of specific genes was normalized to porcine GAPDH as a housekeeping gene ...
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bioRxiv - Cell Biology 2023Quote: ... and data were analyzed by the REST Software (Qiagen). Relative expression of specific genes was normalized to human GAPDH as a housekeeping gene ...
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bioRxiv - Genetics 2020Quote: ... and statistical analyses were performed using REST 2009 software (Qiagen, Hilden, Germany). Genes were defined as downregulated in the mutant strain if the ratio of their transcript level in the mutant strain compared with that in the wild-type strain showed a-fold change > 0 and < 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and reference genes verified using the Pfaffl method and REST software (QIAGEN).
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bioRxiv - Genomics 2020Quote: ... The rest of the sample’s beads were resuspended in 700µL Qiazol (Qiagen), and RNA was extracted using the miRNeasy mini kit ...
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bioRxiv - Molecular Biology 2019Quote: ... the rest of lysate was incubated on a Ni-NTA agarose resin (QIAGEN) at 4 °C with rotation ...
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bioRxiv - Microbiology 2019Quote: ... and rest of the isolation procedure was performed using miRNeasy mini kit (Qiagen).
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bioRxiv - Cell Biology 2020Quote: ... The rest of the supernatant was added to 100 µl Ni-NTA agarose (Qiagen) slurry and incubated for 2.5 hours at 4 °C on a rotary wheel ...
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bioRxiv - Immunology 2022Quote: ... The rest of the extraction was performed using the RNeasy Minikit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... The rest of PCR products were purified using the MinElute PCR Purification Kit (Qiagen, 28004). Sanger sequencing was performed using the OR2W5 forward PCR primer (5’-TCGGCCTGGACTGGAGAAAA-3’) ...
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bioRxiv - Developmental Biology 2020Quote: ... the rest of the embryo was fixed in 4% paraformaldehyde/PBS and stained with DAPI (Qiagen) for easy visualization of the somites under a microscope and characterization of the embryonic stage ...
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bioRxiv - Microbiology 2019Quote: ... The relative expression analysis was performed using the Relative Expression Software Tool (REST, Qiagen Inc., Valencia, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fold changes of N transcript expression with each treatment were calculated corcenning to the Virus control using REST 2009 program (v2.0.13, Qiagen, GbmH) [26] ...
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bioRxiv - Physiology 2019Quote: ... Gene expression data was analyzed using nonparametric tests in REST 2009 Software (M. Pfaffl, Technical University Munich, and QIAGEN). A two-way ANOVA was used to analyze torque as well as fatigue resistance after PGC-1α transfection (group by treatment) ...
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-His6 (34650; IF: 1/500) from Qiagen, mAb anti-GAPDH (GTX28245 ...
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bioRxiv - Bioengineering 2021Quote: The changes in mRNA levels were compared by Relative Expression Software Tool (REST® 2009 v2.013) using 2-ΔΔCT relative quantitative analysis and also confirmed by GeneGlobe Data Analysis Center (Qiagen). Student t test has been used throughout the study ...
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bioRxiv - Developmental Biology 2020Quote: ... Crossing point (Cp) values were obtained and converted to relative mRNA expression levels using the Relative Expression Software Tool (REST) 2009 (Qiagen)52 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The extent of DNA amplification was estimated by dividing the average sequence coverage of the amplifications by the average sequence coverage of the rest of the chromosome in CLC Genomic Workbench (Qiagen) as described previously (Nicoloff et al ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Ct values of the technical replicates were averaged and used for relative gene expression analysis in the REST 2009 software (Qiagen, Germany) [46] ...
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bioRxiv - Immunology 2020Quote: ... the supernatants were collected and a portion the cells were used for flow cytometry analysis and the rest lysed and homogenized in buffer RLT Plus (Qiagen; Hilden, Germany) supplemented with 143 mM β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Recombinant plasmids were then isolated (MiniPrep, Qiagen) and sequenced (DNA Sequencing Facility ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant plasmids were purified with EndoFree Plasmid Maxi Kit (12362, QIAGEN). Oligonucleotide sequences and further details are provided in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Uhrf1 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 40,920 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Swi6 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 45,330 cm-1 M-1) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant HCMVs were reconstituted from HCMV BAC DNA by Superfect (Qiagen) transfection into permissive MRC-5 cells.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was purified by Ni-NTA affinity chromatography column (QIAGEN).
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bioRxiv - Microbiology 2019Quote: ... Recombinant protein in the supernatant was purified via Ni2+-NTA (Qiagen, UK) column [20].
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were purified from lysates on Ni-NTA columns (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Bioengineering 2023Quote: ... Recombinant plasmids were prepared by using the QIAprep Spin Miniprep Kit (QIAGEN) and confirmed by sequencing analysis ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Plant Biology 2020Quote: ... Recombinant proteins were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen) and elution with imidazole ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant His-tagged GAPDH was purified using Ni-NTA Agarose (Qiagen, Valencia, USA) in native conditions according to the manufacturer’s recommendations ...