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Citations for Qiagen :
401 - 450 of 10000+ citations for QuantiFluo Protein Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Microbiology 2022Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... His6-tagged protein was affinity purified using NiNTA resin (Qiagen), which was washed with binding buffer ...
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bioRxiv - Immunology 2019Quote: ... Proteins were purified by affinity chromatography on NiNTA agarose (Qiagen), followed by FPLC on MonoQ (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was isolated by Ni-NTA affinity chromatography (Qiagen) and eluted with 50 mM Tris buffer pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: Protein purification was done using Ni-NTA Spin Columns (Qiagen) following the supplier recommendations ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (Qiagen) and eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-NTA agarose resin (Qiagen), washed with 20 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: ... and His6-tagged protein was captured on NiNTA agarose (Qiagen). Following extensive washes with core buffer supplemented with 20 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were affinity purified on Ni-NTA agarose beads (Qiagen). Proteins eluted from Ni-NTA beads by stepwise addition of increasing concentrations of 50-300 mM imidazole in a buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Immunology 2021Quote: ... and the resulting proteins were purified using Ni-resins (Qiagen). The vector encoding a hexa-His tag fused to a single chain homotrimeric mLIGHT extracellular domain (G73-V239 ...
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bioRxiv - Bioengineering 2022Quote: ... Pan-Fzd protein was purified using Ni-NTA Agarose (Qiagen), followed by biotinylation and size-exclusion chromatography with a Superdex S75 column (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... KASH5 fusion proteins were purified through consecutive Ni-NTA (Qiagen), amylose (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were bound by Ni-NTA agarose (Qiagen) and eluted with 500 mM imidazole in HSB ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... for GST-tagged protein or Ni-NTA Agarose beads (QIAGEN) for His-tagged protein ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for GST-tagged protein or on Nickel beads column (Qiagen) for 6XHIS-tagged protein ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Plant Biology 2023Quote: ... Protein alignment was performed with CLC Genomics Workbench 22.0.1 (Qiagen) using MUSCLE v3.8.425 (Edgar ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with Ni-NTA Agarose (Qiagen, Cat#30230) resin for 30 min at 4 °C in a beaker with stirring ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by Ni2+-affinity (Ni-NTA agarose, Qiagen) then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Microbiology 2023Quote: Plasmids used for protein purification were based on pQE70 (Qiagen). For purification of EsaDEG ...
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bioRxiv - Immunology 2024Quote: ... The PELO protein was purified with Ni2+-NTA-agarose (Qiagen) and followed by further purification by Superdex 200 10/30 prepacked column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The protein was isolated using a Ni-NTA column (Qiagen). 25 μg of isolated protein was used to immunize mice by subcutaneous injection with complete Freund’s adjuvant and boosted three times with incomplete Freund’s adjuvant to generate PfATP4 antibody.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by affinity-purification with amylose resin (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... kit (Qiagen). Phylogenetic groups were determined as described in (Clermont ...
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bioRxiv - Microbiology 2019Quote: ... Kit (QIAGEN) followed with 16S-ITS PCR as previously described (64 ...
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bioRxiv - Genetics 2020Quote: ... Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Kit (Qiagen). Whole-genome sequencing was performed using the Illumina Nextera XT library protocols and sequenced at 2 x 300bp read length on the MiSeq platform (Ramaciotti Centre for Functional Genomics ...
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bioRxiv - Microbiology 2024Quote: ... Kit (Qiagen) using 5 mL culture of V_227 which was grown in MMT-YE medium (20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The kits for RNA extraction were from Qiagen (RNeasy Mini Kit and RNeasy Micro Kit). The reverse transcription kit ...
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bioRxiv - Molecular Biology 2021Quote: ... These assays were run against cDNA replicates with the miRCURY SYBR Green Mastermix (Qiagen) on a Qiagen RotorGene Q qPCR instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... The primers were designed by use of the Pyromark Assay Design Software package (Qiagen), and 10 CpG sites of the FOXP3 intron region were selected for quantitative DNA methylation analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... and miRNA qPCR was performed using miRCURY LNA miRNA PCR Assays (QIAGEN, Germantown, MD) in QuantStudio 6 Real-Time PCR cycler system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... All data were normalized to the housekeeping gene 18S (18S QuantiTect Primer Assay; Qiagen).
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bioRxiv - Biochemistry 2022Quote: ... IL −6 and TNFα mRNA were detected by validated QuantiTect primer assays 144 (Qiagen).
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bioRxiv - Immunology 2023Quote: ... was performed in duplicates using predesigned primer sets (Quantitect Primer Assays, Qiagen, Germantown, MD). Relative mRNA expression between groups was analyzed by the delta-delta Ct method and normalized to housekeeping genes ...
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bioRxiv - Genetics 2023Quote: ... with biotin-labeled methylation-specific primers designed with Pyromark Assay Design Software 2.0 (Qiagen). The primer sequences are shown in Supplemental Table S1 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... resuspended in nuclease- free water and quantified using Qubit DNA Broad Range assay (Qiagen). The duplex ddPCR quantification assay was based on the co-amplification of two target genes each detected with specific Taqman probes ...
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bioRxiv - Cell Biology 2024Quote: ... All qPCR assays were performed on Rotor-Gene Q thermocycler (Qiagen, Toronto, ON, Canada). The relative expression of mRNA and miRNA was normalized to ACTB and snRNA U6 ...
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bioRxiv - Microbiology 2024Quote: The multiplex real-time qPCR assays were carried out in Rotor-Gene Q (Qiagen). A primer mix (200 µl ...
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bioRxiv - Immunology 2024Quote: ... gapdh (Assay ID: Mm99999915_g1) on the Rotor-Gene Q real-time PCR cycler (Qiagen). Thermal cycling conditions included an initial denaturation step at 95°C for 10 min followed by 40 cycles of 95°C for 15s and 60°C for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... Using a commercial kit (QIAGEN DNeasy isolation kit #69506), DNA was isolated from a tissue punch taken from the external ear ...