Labshake search
Citations for Qiagen :
1 - 50 of 1254 citations for Protease Inhibitor Cocktail EDTA Free 100X in DMSO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... and complete mini EDTA free protease inhibitor followed by mechanical homogenation by Tissue Ruptor (Qiagen). The samples were centrifuged at 13000 rpm for 15 mins and protein concentration calculated by BCA ...
-
bioRxiv - Physiology 2021Quote: ... 1X protease inhibitor cocktail) using TissueRuptor (Qiagen). Isolated epithelial cells were also lysed in RIPA buffer ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing protease inhibitor cocktail using PowerLyser 24 (Qiagen) and mixed gently at 4 °C for one hour ...
-
bioRxiv - Genetics 2020Quote: ... supplemented with protease inhibitors and EDTA) volume using TissueLyzer equipment (Qiagen). Lysates were incubated on ice 20 minutes followed by centrifugation at 20,000xg for 20 minutes at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... protease inhibitor tablet (Qiagen) and the nuclease 0.0002% Benzonase (Qiagen ...
-
bioRxiv - Immunology 2019Quote: ... protease inhibitor tablet (Qiagen) and the nuclease 0.0002% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
-
Glutaric aciduria type 3 is a naturally occurring biochemical trait in inbred mice of 129 substrainsbioRxiv - Biochemistry 2020Quote: ... were homogenized in ice-cold RIPA buffer supplemented with protease inhibitor cocktail (∼20 mg tissue per 1 mL lysis buffer) using a TissueLyser II apparatus (Qiagen) and the homogenates were centrifuged at 1,000xg ...
-
bioRxiv - Immunology 2020Quote: ... 50 mM Tris-HCl pH 8.0) with 0.1 μL Protease Inhibitor Cocktail VI (Astral Scientific No. P-1515) using the TissueLyser II (Qiagen No. 85300) at 25 Hz for 2 min twice ...
-
bioRxiv - Pathology 2023Quote: ... tissue and cells were lysed in RIPA lysis buffer supplemented with orthovanadate and a cocktail of protease inhibitors at 4°C (using a TissueLyser (Qiagen) to homogenize liver tissue ...
-
bioRxiv - Neuroscience 2024Quote: ... with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and TissueRuptor II (Qiagen) homogenization ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 mg of frozen brain was taken per mouse sample and lysed with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and was homogenised using a TissueRuptor II (Qiagen). Zebrafish larvae were similarly extracted ...
-
bioRxiv - Biochemistry 2024Quote: ... and 1% Protease Inhibitor using TissueLyser II (Qiagen) and 5 mm stainless steel beads (Qiagen ...
-
bioRxiv - Physiology 2019Quote: ... 1μM MG132 and protease inhibitors) using a TissueLyser II (Qiagen). After centrifugation at 13000 rpm for 15 min ...
-
bioRxiv - Genomics 2019Quote: Total RNA was isolated from Arabidopsis seedlings grown for 10 days and exposed to DMSO or splicing inhibitors for 6 or 24 hours with RNeasy Plant Mini Kit (Qiagen) according to manufacturers’ instructions ...
-
bioRxiv - Genomics 2019Quote: KARPAS-422 cells treated with DMSO or EZH2 inhibitor were harvested and total RNA was isolated with Qiagen RNeasy Plus Mini kit (Qiagen 74136). Downstream sample processing ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.1 % SDS) containing protease inhibitors and 0.1mM of 1,10- phenanthroline using a TissueLyser II (QIAGEN). The samples were then quantified ...
-
bioRxiv - Neuroscience 2021Quote: ... and Halt Protease inhibitor (Thermo-Scientific) (1:100) for 1min at 30Hz using the TissuelyserLT (Qiagen). Homogenates were transferred to chilled Eppendorf tubes (−20°C ...
-
bioRxiv - Microbiology 2019Quote: ... protease and phosphatase inhibitors) or RLT buffer supplemented with “- mercaptoethanol (RNeasy mini kit, Qiagen, Hilden, Germany) for western blot analysis and total RNA extraction ...
-
bioRxiv - Genomics 2023Quote: ... After ligation nuclei were pelleted and resuspended in cold PBS with DAPI to a final concentration of 300nM and GFP+ cells were FAC-sorted into a 96 well plate with 2ul lysis buffer (20mM Tris pH 8, 20mM NaCl, 0.15% Triton X-100, 25mM DTT, 1mM EDTA, 500nM Carrier ssDNA, and 15ug/mL Qiagen Protease) and lysed for 1 hour at 50°C and inactivated 15 minutes at 70°C ...
-
bioRxiv - Genomics 2023Quote: ... then stopped with transposome removal buffer (300nM NaCl, 45 mM EDTA, 0.01% Triton X-100 with 100ug/mL Qiagen Protease) and incubated at 50°C for 40 minutes and 70°C for 20 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.5% deoxycholic acid with a protease inhibitor) and pre-cooled tissue homogenizer (TissueLyser LT, Qiagen GmbH, Hilden, Germany) for 2-4 min at 45 Hz ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue was homogenized in 150uL of RIPA buffer with protease and phosphatase inhibitors by bead homogenizer (Tissue LyserII, Qiagen) for 3 mins at a frequency of 30 hz/s and immediately cooled for 5 mins on ice ...
-
bioRxiv - Pathology 2020Quote: 200 microgram of kidney tissue were homogenized and lysed by 8M urea in 20mM HEPES (pH=8.0) buffer with protease and phosphatase inhibitors using Tissue Lyser II (QIAGEN). Samples were reduced and alkylated followed by MS-grade trypsin digestion ...
-
bioRxiv - Microbiology 2020Quote: ... and an RNase-free DNase-free Kit (Qiagen). The RNA sample was reverse transcribed with Transcriptor Universal cDNA Master (Roche Diagnostics ...
-
bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Brain and DRG samples from up to four donors per species were homogenized in Tissue Protein Extraction Reagent (T-PER) supplemented with HALT protease inhibitor and benzonase endonuclease using a Tissue Lyser Mixer Mill Grinder (Qiagen), while serum and CSF were directly diluted in supplemented T-PER ...
-
bioRxiv - Immunology 2020Quote: ... BSA free (Qiagen; 34670 ...
-
bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors (PMSF, Aprotinin, Pepstatin, Leupeptin) and homogenised with autoclaved metal beads in a TissueLyser II instrument (Qiagen, Hilden, Germany), followed by ultrasonication (8 sec ...
-
bioRxiv - Molecular Biology 2023Quote: ... DMSO or 5-aza-dC-treated cells (51304, Qiagen, DUS, GER). 200 ng DNA was dropped onto a positively charged Nylon membrane (Amersham Biosciences ...
-
bioRxiv - Genomics 2022Quote: ... 10 μL of protease mix (100 μg protease (Qiagen, 19155), 1x GC reaction buffer ...
-
bioRxiv - Genomics 2022Quote: ... 400 nL of protease mix (6 μg protease (Qiagen, 19155), 6.25x NEBuffer 4 (NEB ...
-
bioRxiv - Molecular Biology 2019Quote: ... with iTAqSYBR Green Supermix with ROX (Bio-Rad).RNase-free DNase (Qiagen)-treated total RNA was used for cDNA synthesis using iScript cDNA synthesis kit (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: ... RNase-free DNase (Qiagen) treatment was performed on each sample ...
-
bioRxiv - Microbiology 2022Quote: ... DMSO-treated or MTX-treated RAW264.7 cells with RNeasy MinElute Kit (Qiagen) and reverse transcribed using SuperScript III First-Strand Synthesis SuperMix (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2020Quote: ... and Qiagen Protease (Qiagen), then incubating the samples at 50°C for 2 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... CpdG or DMSO treated cells with the RNAeasy Mini kit (Qiagen cat #: 74104) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Endotoxin-free plasmid maxipreps (Qiagen) were made for rAAV preparations.
-
bioRxiv - Immunology 2021Quote: ... and RNase-free DNase (Qiagen). All samples showed an A260/280 ratio > 1.99 ...
-
bioRxiv - Microbiology 2021Quote: ... and RNase-Free DNase (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... and Nuclease-free water (Qiagen) were mixed to total volume 12 μl ...
-
bioRxiv - Plant Biology 2022Quote: ... RNase-Free DNase Set (Qiagen) was used to remove DNA ...
-
bioRxiv - Neuroscience 2021Quote: ... Endotoxin free plasmid maxipreps (Qiagen) were used for AAV preparations ...
-
bioRxiv - Immunology 2022Quote: ... RNase-free DNase Set (Qiagen) was used to remove potential DNA contamination ...
-
bioRxiv - Cell Biology 2021Quote: ... with RNase-free DNase (Qiagen). RNA was amplified ...
-
bioRxiv - Plant Biology 2021Quote: ... RNase-Free DNase Set (Qiagen) was used according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... in RNAse free water (Qiagen)) ...
-
bioRxiv - Neuroscience 2022Quote: ... RNAse-free DNAse I (Qiagen) was used to remove traces of genomic DNA.