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Citations for Qiagen :
501 - 550 of 5358 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
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bioRxiv - Molecular Biology 2020Quote: CSF cell precipitates (approximately 5000 cells) was resuspended in 700 μl of QIAzol Lysis Reagent (Qiagen, Germany) and stored at −80°C ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was isolated from 4×107 KPC cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen). NGS libraries were prepared using the following primers:
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bioRxiv - Neuroscience 2022Quote: FACS sorted microglia cells were flash frozen in dry ice into 300 μL RNAprotect cell reagent (Qiagen). Total RNA was extracted using RNeasy mini kit (Qiagen) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293FT cells were transfected using calcium phosphate method and U2OS cells using Effectene Transfection Reagent (Qiagen). To induce primary cilia ...
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bioRxiv - Genomics 2019Quote: ... Cell pellets were either processed immediately or kept at −80°C in RNAProtect Cell Reagent (QIAGEN # 76526).
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bioRxiv - Cell Biology 2020Quote: Cell pellets were thawed for genomic DNA extraction using Blood & Cell Culture DNA Midi/Maxi Kit (Qiagen). PCR was performed with Q5 Hot Start High-Fidelity 2 × Master Mix (#M0494L ...
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bioRxiv - Cancer Biology 2019Quote: Genomic DNA was purified from dissociated tumors or cell pellets using the Gentra Puregene Cell kit (Qiagen). A 1,122 bp amplicon ...
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bioRxiv - Genetics 2020Quote: ... cell pellets (10*106 cells) were collected and RNA was extracted using the RNeasy Mini Kit (Qiagen). RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... Worms were lysed in Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol at 55°C for 2 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of gDNA was extracted from WILD-seq transduced cells (Blood&Cell Culture DNA Kit, Qiagen) and the barcode amplified by PCR using primers containing Illumina-compatible adapters (gDNAWhitelist_Fwd/Rev) ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was harvested by washing cells with PBS and lysing cells with buffer RLT plus (Qiagen, 1053393) supplemented with 1% beta-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were collected for genomic DNA extraction using red blood cell lysis solution (Qiagen, 28606).
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bioRxiv - Microbiology 2022Quote: ... 900µl cell samples were pelleted and RNA was extracted from cells by using the RNeasy kit (Qiagen). The resulting total RNA sample was diluted to 1μg of total RNA in 10μl of H2O and treated with ezDNASE (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: RNA from FACS-isolated cell populations or cell cultures were isolated using the RNeasy mini kit (Qiagen) with on-column DNAse digestion according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... cells were collected for genomic DNA (gDNA) extraction using the Blood & Cell Culture Midi kit (Qiagen, #13343) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cells or HeLa FlpIn cells were transfected with siRNAs at 100 nM using the HiPerFect (Qiagen), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... total DNA from 1×106 cells was extracted using the Blood & Cell Culture DNA Mini Kit (Qiagen). Extracted DNA was diluted to 10 ng/μl and subjected to qPCR analysis ...
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bioRxiv - Cell Biology 2024Quote: HAEC cells from the stretch experiments were harvested by cell scraping and resuspended in RLT buffer (Qiagen) supplemented with β-Mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from CD11b+ cells and LK cells using RNeasy plus mini kit (Qiagen 74134) [source?] ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were isolated from cell pellets using the EndoFree Plasmid Maxi Kit (0.45 g cells/column; Qiagen, 12362). Sequencing-based barcode quantification (see below Quantifying barcodes from plasmids and rabies virus genomes ...
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA from control cells and trRpn13 MM cells were extracted by QIAamp→ DNA Mini Kit (51304; Qiagen). DNA editing status was validated by performing TOPO blunt cloning of PCR amplicons encompassing the guide RNA target sites and Sanger sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ magnetic sorted microglia cells or embryonic neural progenitor cells were homogenized in QIAzol Lysis Reagent (#79306, QIAGEN) either immediately after isolation or after 10 days of in vitro culturing ...
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bioRxiv - Cell Biology 2021Quote: Cell pellets of 100,000 cells were prepared and RNA was extracted using the RNeasy Mini Kit (Qiagen, #74104). Stranded mRNA (polyA-selected ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from cell pellets (<1×107 cells) using the RNeasy Mini kit (QIAGEN, cat. 74134). Cells were lysed with RLT buffer (with no ß-ME ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from MCF-7 cells (1×106 cells/well) was isolated using RNeasy Mini kit (74106; Qiagen), and 500ng cDNA was synthesized with random hexamers by reverse transcription (SuperScript III ...
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bioRxiv - Neuroscience 2022Quote: ... the cell viability was assessed and cells were then resuspended in 350µl RLT Lysis buffer (Qiagen; Cat #: 74136) containing 2-Mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed 3 times with PBS and lifted with 500µL/well of Cell Protect Reagent (Qiagen) for 5 min ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted using the Blood & Cell Culture DNA Mini (<5M cells) Kits (Qiagen, cat. no. 13323) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... cells were harvested and genomic DNA was isolated using the “Blood & Cell Culture DNA Mini Kit” from Qiagen and according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA was prepared from cultured tumor cells or sorted cells from implanted tumors using RNeasy Mini Kit (QIAGEN). cDNA was generated using High-capacity cDNA Reverse Transcription Kit (Bio-Rad) ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Cancer Biology 2019Quote: ... and Treg (DAPIneg, i.v. neg, Thy1.2+CD4+Foxp3-GFP-positive) cells were single-cell sorted into Buffer TCL (Qiagen) plus 1% B-mercaptoethanol in 96-well plates using a MoFlo Astrios cell sorter ...
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bioRxiv - Cell Biology 2020Quote: RNA was collected from siRNA-treated HeLa cells and extracted using the RNeasy PowerLyzer Tissue & Cells Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: DAFhi and DAFlo GC B cells (CD19+ CD20+ CD38+ IgD−) were resuspended in RLT cell lysis buffer (Qiagen) after flow cytometric sorting ...
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bioRxiv - Cancer Biology 2022Quote: ... Each population of filtered cells as well as nonfiltered nontreated cells were lysed using a QIAshredder Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Each population of filtered cells as well as nonfiltered nontreated cells were lysed using a QIAshredder Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the Blood & Cell Culture DNA Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... genomic DNA was isolated from HEK293T cells 3 days after transfections using the Gentra Puregene Cell Kit (Qiagen) and was diluted to 10 ng/μL ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from sorted cell populations of greater than 200,000 cells was extracted using the DNeasy Kit (Qiagen). DNA from cell populations less than 200,000 cells was processed via direct lysis using DirectPCR lysis buffer (Viagen Biotech) ...
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bioRxiv - Physiology 2024Quote: ... The GFP+ and GFP− cells as remaining cells were either kept in QIAzol Lysis Reagent (QIAGEN, Valencia, CA), and stored at −80°C or immediately used for single cell analysis.
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bioRxiv - Cell Biology 2023Quote: ... cells (1 x 106 cells/well) were washed three times with PBS and lysed in Buffer RLT (Qiagen). Lysates were transferred to Eppendorf tubes on ice and stored at -80°C until ready for RNA purification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... regius cells harboring cyanobacterial symbionts was subjected to independent genome amplification using REPLI-g Single Cell Kit (QIAGEN), and the amplified DNA samples were processed with S1 nuclease (TaKaRa ...
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bioRxiv - Cell Biology 2020Quote: ... for MDCK cells and Effectene (Qiagen # 301425) for A6 cells following the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with siRNAs (FlexiTube, Qiagen) using Lipofectamine RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were subjected to RBC lysis (Qiagen) according to the manufacturers protocol and used for the further steps described below ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were lysed with RLT buffer (Qiagen) and frozen at −80 for downstream RNA isolation ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell transfection was performed using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in QIAzol reagent (Qiagen) for RNA isolation or fixed with 4% paraformaldehyde (PFA ...