Labshake search
Citations for Qiagen :
251 - 300 of 5357 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: gDNA was isolated from human sarcoma models and controls using the QIAamp DNA Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from human sarcoma models and controls using the RNeasy Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
-
bioRxiv - Genetics 2024Quote: ... and aligned to a human reference sequence GRCh38/hg38 by using CLC Genomics Workbench v20 (Qiagen). The TPM (transcript per million ...
-
bioRxiv - Molecular Biology 2021Quote: ... UVSS and CS primary skin fibroblasts was isolated using the QIamp® DNA mini kit (Cat#51304; Qiagen) and used for microarray-based analysis of genome wide DNA methylation levels in two different sets (see Fig.1A for detail of cells used for each set) ...
-
bioRxiv - Cancer Biology 2020Quote: Genomic DNA extraction was performed in 26 primary mammary tumors using a QIAamp FFPE kit (Qiagen, Dusseldorf, Germany) following the manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2019Quote: ... 10-15 μl of primary or re-amplified WTA product was purified using QIAquick PCR Purification Kit (QIAgen) according to the manufacturer’s instruction with several changes ...
-
bioRxiv - Genomics 2022Quote: ... sampled primary leaf of each plant and extracted DNA with DNeasy Plant Mini Kit (Qiagen KK, Tokyo, Japan).
-
bioRxiv - Microbiology 2022Quote: ... Primary-pools of ants were homogenized with 2 ceramic beads (2.8mm) in 350 µl QIAzol Lysis Reagent (Qiagen) for 2×2min at 30Hz in a TissueLyser II (Qiagen) ...
-
bioRxiv - Neuroscience 2020Quote: ... frozen fragments of human cortex were homogenized with a motorized hand pestle in RLT lysis buffer (Qiagen) and qPCR was performed as described in qPCR section.
-
bioRxiv - Cancer Biology 2020Quote: Total genomic DNAs (containing human and viral genomes) were isolated using AllPrep DNA/RNA Mini Kit (Qiagen) from the NPC biopsy ...
-
bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... Genomic human DNA was isolated from whole blood using QIAamp DNA Blood Mini Kit from Qiagen (51106) and phosphodiester DNA from TIB Molbiol ...
-
bioRxiv - Paleontology 2019Quote: Human 2 and 3: DNA was extracted from bones using QIAamp® DNA Investigator kit (56504, Qiagen). Bones were thoroughly washed (X5 ...
-
bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
-
bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... Human ATGL-targeting and cPLA2α-targeting siRNAs and the AllStars Negative Control siRNA were from Qiagen (Germany). T863 (DGAT1 inhibitor) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Rattus norvegicus reference genome Rnor_6.0) or the human reference genome (Homo sapiens reference genome GRCh37) using CLC Genomics Workbench 20.0.4 (Qiagen). Only reads with <2 mismatches and minimum length and a similarity fraction of 0.8 were mapped to the reference genome ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA of human macrophages was isolated according to manufacturer’s instructions using the RNeasy extraction kit (Qiagen). mRNA was extracted using the Next Poly(A ...
-
bioRxiv - Genetics 2024Quote: The RNA quality of human kidney FFPE sample was checked by RNeasy FFPE kit (Qiagen-Cat #73504) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: ... the effect of human rRNA removal was also assessed using a QIAseq FastSelect rRNA removal kit (Qiagen). The rRNA removal reaction was performed after RNA thermal denaturation at 95℃ 5 min according to the instructions.
-
bioRxiv - Neuroscience 2021Quote: RNA was extracted from primary tissue cultures using the RNeasy Plus mini kit (cat no. 74136, Qiagen, Hilden Germany). Reverse transcription was performed using Invitrogen SuperScript IV First Strand Synthesis System with Oligo dT primers according to the manufacturer’s protocol (cat no ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
-
bioRxiv - Genetics 2023Quote: ... The mRNA from tissues or primary myoblast cultures was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen, GmbH). RNA concentration was estimated by measuring the absorbance at 260 nm using Nanodrop (Thermofisher ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sequence reads were mapped against the human reference genome (NCBI build 37/hg19) using CLC Genomics Workbench (version 5.0.1) software (QIAGEN). Mutation analysis was performed under recessive ...
-
bioRxiv - Neuroscience 2021Quote: ... microdissected human brain tissue was flash frozen and lysed to extract proteins using a TissueLyser II system (QIAGEN) and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225) ...
-
bioRxiv - Microbiology 2019Quote: Microbial genomic DNA was extracted from the human stool samples using the DNeasy PowerSoil DNA Isolation Kit (Qiagen). The V4 region of 16S rRNA gene was amplified and sequenced using the Illumina MiSeq platform(67) ...
-
bioRxiv - Molecular Biology 2019Quote: ... in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT) (RT2 qPCR Primer Assays, Qiagen) mRNA when performing siRNA analyses.
-
bioRxiv - Genetics 2019Quote: ... The list of human orthologs was analyzed for pathway enrichment by Ingenuity Pathway Analysis software (Qiagen, Hilden, Germany).
-
bioRxiv - Cell Biology 2019Quote: ... transfected with 100 ng of human TfR (and 100ng of rescue construct if applicable) using the Effectene (QIAGEN) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... human HDL (40nM) or PBS for 48 hours prior to RNA isolation using the RNeasy Mini kit (Qiagen). RNA samples were converted to cDNA libraries by the Northwestern University Genomics Core facility and were then run on the Illumina HT-12 microarray ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from 22 human vaginal samples using the QIAamp UCP Pathogen Mini Kit (QIAGEN, Venlo, Netherlands) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... genomic DNA from murine or human monocytes was isolated using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, 80224). DNA (1 µg ...
-
bioRxiv - Neuroscience 2021Quote: The total RNA from mouse cortex and human PBMC was extracted using the RNeasy® Mini Kit (Qiagen) and real-time PCR was done as described in the Supplementary material.
-
bioRxiv - Neuroscience 2020Quote: ... RNA was isolated from human autopsy brain tissue using the RNeasy Plus Universal Mini Kit (Qiagen Cat# 73404).
-
bioRxiv - Immunology 2020Quote: ... RT2 profiler PCR array of human NF-κB signaling targets was performed as instructed by the manufacturer (Qiagen).
-
bioRxiv - Genomics 2021Quote: ... The manufacturer’s protocol was followed for manual purification of total RNA from human whole blood (Qiagen, cat #762164). RNA was eluted with RNase free water ...
-
bioRxiv - Neuroscience 2021Quote: Microdissected mouse and human brain tissue was flash frozen and homogenized in Trizol using a TissueLyser II (Qiagen). Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN ...
-
bioRxiv - Neuroscience 2023Quote: ... Bulk gDNA and RNA from human retinal organoids were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen) or DNeasy Blood & Tissue Kit (Qiagen ...
-
bioRxiv - Cell Biology 2023Quote: SiRNAs targeting human MSI2 (Supp Table S3) and nonspecific control pool siRNAs were purchased from Qiagen (Frederick, MD). Cultured cells at 50% confluence were transfected with siRNA at final concentrations of 50 nmol/L using the Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2023Quote: Total RNA was extracted from the frozen human stomach tissues using the RNeasy plus Mini Kit (Qiagen, US) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Reads were aligned to the GRCm38 build of the human genome by using CLC Genomics Workbench v10 (Qiagen).
-
bioRxiv - Cell Biology 2023Quote: Total RNA from the human biopsy samples and mice colon tissue was isolated using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen). Transcript abundance was expressed as reads per kilobase of transcript per million mapped reads (RPKM) ...
-
bioRxiv - Cell Biology 2022Quote: Mouse Plin2 homology arms were obtained by extracting DNA from mouse primary mouse NSPCs from the SVZ using DNeasy Blood & Tissue Kit (#69506, Qiagen). The sequences covering the sgRNA target site were extracted from the genomic DNA using PCR ...
-
bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from AAV1-transduced primary hippocampal neurons (DIV12-14) using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Total RNA was polyA selected and sequenced (Hudson Alpha GSL ...
-
bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
-
bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from 100 mg of apical parts of primary inflorescences of exo70a2 and WT plants using the RNeasy kit (Qiagen). Prior to reverse transcription ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA from primary hepatocytes and organoids was isolated following Trizol Reagent RNA extraction method coupled with RNaesy MinElute columns (Qiagen). cDNA was synthetized using an iScript cDNA Synthesis Kit from Bio-Rad ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA from 5-20 mg of melanoma primary and metastatic tissues was extracted using AllPrep DNA/RNA Mini isolation kit (Qiagen). A total of 100 ng of total RNA was used as input for RNA- seq libraries using SMARTer Stranded Total RNA-seq - Pico input (Takara Bio USA ...