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Citations for Qiagen :
1 - 50 of 5407 citations for Primary Human Cardiac Fibroblast Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated from cardiac fibroblasts using RNeasy Mini Kit (Qiagen Inc., Valencia, CA). First-strand cDNA was synthesized from total RNA (10ng/μl ...
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bioRxiv - Biochemistry 2020Quote: Total RNA was extracted from cardiac fibroblasts and myocytes using RNeasy® Micro kits (Qiagen Inc.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... primary T cells and BJ-5at fibroblasts using the RNeasy Plus Mini Kit (Qiagen. #74136), reverse transcribed to first-stand cDNAs using iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts harvested in passage 3 using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from primary fibroblasts was extracted using the RNeasy Mini Kit (Qiagen), including DNAse I treatment to remove genomic DNA contamination ...
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bioRxiv - Genetics 2023Quote: ... primary fibroblasts and brain tissue was isolated using the Gentra Puregene kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from human primary cells using AllPrep RNA/RNA/miRNA universal kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from human primary immune cells using the RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... UVSS and CS primary skin fibroblasts was isolated using the QIamp® DNA mini kit (Cat#51304; Qiagen) and used for microarray-based analysis of genome wide DNA methylation levels in two different sets (see Fig.1A for detail of cells used for each set) ...
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bioRxiv - Molecular Biology 2024Quote: DNA was isolated from fibroblast cells using the Puregene Kit (Qiagen) according to instructions ...
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bioRxiv - Molecular Biology 2020Quote: FACS sorted cardiac ECs were homogenized using QIA shredder (Qiagen) and purified using RNeasy Plus Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2019Quote: ... hTERT-immortalized fibroblasts (Qiagen RNeasy) or whole blood (PAXgene Blood RNA Kit ...
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bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from human fibroblasts as well as iNs from the same lines using the miRNeasy kit (Qiagen) followed by Universal cDNA synthesis kit (Fermentas) ...
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bioRxiv - Genomics 2020Quote: ... and (iii) fibroblast cell lines using the DNeasy Blood and Tissue Kit (QIAGEN) that included an RNAse A treatment step (QIAGEN ...
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bioRxiv - Genomics 2020Quote: ... and (iii) fibroblast cell lines using the DNeasy Blood and Tissue Kit (QIAGEN) that included an RNAse A treatment step (QIAGEN ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells using Rneasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from sorted infected primary human hepatocytes using miRNeasy Micro Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from ∼1000 iDANoids (containing 2000 seeded fibroblasts each) or from a T25 flasks (1.2 × 106 fibroblasts plated) using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was isolated from Mouse Embryonic fibroblast cells (MEF) using QIAmp DNA mini kit (Qiagen), PCR amplified and subsequently cloned in vector pEGFPC1 with a constitutive promoter ...
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bioRxiv - Immunology 2020Quote: ... The RNA was extracted from the cardiac tissues using Allprep Kits (Qiagen, Düsseldorf, Germany), then the RNA sequencing was carried out using the TrueSeq RNA Sample Prep Kit (Illumina ...
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bioRxiv - Genetics 2023Quote: ... total cardiac myocyte RNA was extracted using a miRNeasy Mini Kit (Qiagen, cat # 217006) and treated with DNase I (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Total RNA from PGC fibroblast and DF-1 cells were extracted using an RNeasy mini kit (Qiagen), following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... while macrophages and synovial fibroblasts were sorted based on positivity for Far Red (macrophages) or CellTrace™ Violet (synovial fibroblasts) into RLT buffer (Qiagen) containing 1% β-mercaptoethanol using FACS-ARIA III ...
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bioRxiv - Physiology 2020Quote: Total RNA was isolated from cardiac or liver tissue using an RNeasy® kit (Qiagen) and first-strand cDNA synthesis performed (2 µg total RNA ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from skeletal and cardiac muscle using the miRNeasy kit (217004, Qiagen). Quadriceps muscle and the apex of the heart were used for total RNA ...
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bioRxiv - Cell Biology 2023Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using the RNeasy extraction kit (Qiagen). Two µg of RNA each was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... DNA samples were extracted from PKS skin fibroblasts and normal control skin fibroblasts using the Puregene DNA isolation kit (Qiagen, Valencia, CA) with modified protocol ...
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bioRxiv - Genomics 2020Quote: ... fuscus immortalized skin fibroblasts (Qiagen RNEasy Mini Kit #74104) and converted to cDNA (Maxima First Strand cDNA synthesis kit with dsDNAse #K1672) ...
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bioRxiv - Immunology 2023Quote: ... Cultured fibroblasts were prepared in TCL buffer (Qiagen, 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... Human cell motility RT2 profiler PCR Array (Qiagen) for control-MPC and Givi-MPC was performed ...
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bioRxiv - Bioengineering 2023Quote: Total RNA from cardiac tissue biopsies (2.5 mm) were extracted following the RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was collected from treated human or murine primary spinal cord astrocytes using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from ctrl and HD-iNs converted in T25 flasks (200,000 fibroblasts cells plated for conversion per sample) using DNeasy Blood & Tissue Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... cancer-associated fibroblasts (CAFs) and C666-1 cells using RNeasy Mini kit per the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA integrity number (RIN ...
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bioRxiv - Developmental Biology 2019Quote: RNA from fibroblasts was isolated using RNeasy Mini Kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Fibroblasts were transfected with siRNA using HiperFect transfection reagent (Qiagen). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: Cardiac RNA from male fetuses was extracted using a Qiazol/miRNeasy mini kit protocol (Qiagen, Hilden, Germany). Library preparation for mRNA sequencing followed manufacturer protocol of TruSeq RNA Library Preparation kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... a cancer associated fibroblast (CAF) cell line (pCAF2) expressing TGF-β responsive SMAD2/3/4 RE-Luciferase (Qiagen, #CLS-017L) was created ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized keratinocytes (tKC) or fibroblasts (BJ) were treated for 24 hr then the cells were lysed in RLT buffer(Qiagen) and total RNA purified using the Biomek FxP and the RNAdvance 2.0 Tissue Isolation kit (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... epithelial cells (DAPI-EGFP+CD45-) and fibroblasts (DAPI-EGFP-CD45-CD11b-CD31-CD3-) were sorted and lysed in RLT buffer (Qiagen). Total RNA was prepared using RNeasy (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA from control and SLC25A46 knock-out fibroblast and HeLa cell lines were extracted and purified by using the RNeasy Mini kit (QIAGEN). Three independent biological replicates were collected ...