Labshake search
Citations for Qiagen :
551 - 581 of 581 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... RNA was prepared by phenol/chloroform preparations followed by ethanol precipitation and subsequent treatment with RNase-free DNase I (Qiagen). Photometric analyses of RNA samples all had A260/280 ratios higher than 2.1 ...
-
bioRxiv - Microbiology 2021Quote: ... virion RNA was purified using the RNeasy Mini Kit with on-column DNase digestion with RNase-Free DNase I (Qiagen). The copies/μL in the virion standard were estimated by triplicate measurements using the Abbott RealTime SARS-CoV-2 assay (Abbott m2000 Molecular Platform) ...
-
bioRxiv - Neuroscience 2022Quote: The isolation of total mRNA was performed with the RNeasy Micro Kit and treated with Rnase free Dnase I (Qiagen). 500ng were used to synthesize cDNA with the SuperScript III Reverse Transcriptase Synthesis Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... was used for total RNA extraction according to the manufacturer’s protocol which included a first DNase treatment using the On-column DNase I digestion kit (Qiagen, Switzerland). Extracted total RNA was subjected to a second DNase treatment using Promega RQ1 at 1 unit/μg of RNA ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by three rounds of washes with sterile PBS + PI for 45 mins, then subjected to one round of incubation with DNase I (4716728001, MilliporeSigma) + RNase A (19101, Qiagen) + PI for 72 hours ...
-
bioRxiv - Microbiology 2023Quote: ... Genomic DNA was digested on-column for 15 min at room temperature using the RNase-free DNase I Set (Qiagen). First-strand complementary DNA (cDNA ...
-
Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: 1 × 105 CD8+ CD44LO CD62LHI Qa2HI OT-I GFPLO and GFPHI cells from three biological replicates were sorted into RLT Lysis Buffer (Qiagen) containing 1% 2-mercaptoethanol ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
-
bioRxiv - Cell Biology 2023Quote: ... BioConcept) in TAE buffer (3-07F03-I, BioConcept) and products were extracted using the QIAquick Gel Extraction Kit (28706, Qiagen) and Sanger sequenced by Microsynth (Balgach ...
-
bioRxiv - Cancer Biology 2023Quote: Investigation of the cell cycle related genes in LoVo cell line on treatment with FA FOS I was performed as described above by using the synchronized and 24 h FA FOS I (198 µg/ml) treated cells profiled by using RT2 Prolifer PCR Array- Human Cell cycle from Qiagen. The data was analysed by using Gene globe platform and reactome pathway analysis.
-
bioRxiv - Pathology 2023Quote: ... After DNase I digestion and removing the ribosomal RNAs (rRNAs) from total RNA using the QIAseq FastSelect-rRNA Plant Kit probe (QIAGEN), several Adenine bases were tailed at the 3’ end of the remaining RNAs according to Liefting et al (47) ...
-
bioRxiv - Physiology 2024Quote: ... The resulting cRNA probe was digested with DNase I and purified with the RNeasy MinElute cleanup kit (Qiagen, Hilden, Germany).
-
bioRxiv - Plant Biology 2023Quote: ... patens RNA was extracted and rid of contaminant genomic DNA using RNeasy Plant Mini Kit and on-column DNAse I treatment (Qiagen), following supplier’s indications ...
-
bioRxiv - Cancer Biology 2023Quote: ... NHEJ reporter plasmid was digested with I-Sce1 for 6h and purified using a QIAEX II Gel Extraction Kit (QIAGEN). Exponentially growing cells were transfected using an Amaxa nucleofector with the U-023 program ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from batches of embryos, ICM or TE (n = 20) using PicoPur Arcturus (Excilone, France) with a DNase I (Qiagen, Germany) treatment as recommended by the supplier ...
-
bioRxiv - Cell Biology 2021Quote: ... treated with DNase I at 37°C for 30 min and finally cleaned with the miniElute kit (QIAGEN, Catalog Number: &28004). For next-generation sequencing ...
-
bioRxiv - Cancer Biology 2020Quote: ... The ligated products were size-selected (AMPure Beads, Agencourt, Beverley, MA) and amplified (GeneRead DNA I Amp Kit, Qiagen, Mississauga, ON); then the regions of interest were captured using biotinylated DNA baits (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... residual genomic DNA contamination was removed by an on-column DNAse digestion performed using the RNAse-free DNAse I kit (Qiagen #79254) following the manufacturer’s protocol at 37°C for 1 hr ...
-
bioRxiv - Plant Biology 2019Quote: ... and RNA was extracted following the manufacturer’s instructions using the RNase-free DNase I Set for on-column DNase treatment (Qiagen, Germantown, MD, USA).
-
bioRxiv - Genomics 2020Quote: ... The DNA-seq libraries were constructed from 500 ng of size selected DNA with the GeneRead DNA library I core kit (Qiagen, cat #180434) and GeneRead Adapter I set B (Qiagen ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Total RNA was extracted from frozen tissues using the RNeasy® Plus Mini kit with treatment of DNase I according to the manufacturer’s protocol (Qiagen, Germantown, MD). The integrity of RNA samples were examined by running on agarose gel and using bioanalyzer.
-
bioRxiv - Cancer Biology 2022Quote: ... Total RNA was treated with DNase I (Bangalore Genei, Bangalore, India) as described previously [28] and cleaned using RNeasy mini kit (Qiagen, Hilden, Germany). RNA was quantified by nano-spectrophotometer (Eppendorf ...
-
bioRxiv - Microbiology 2022Quote: ... Dual-index barcodes and adapters were added to amplified products through a second-round of PCR using the QIAseq 16S/ITS 96-Index I array (Qiagen, Hilden, Germany). Cycling conditions were ...
-
bioRxiv - Immunology 2022Quote: RNA from sorted Treg and Tconv cells was extracted using QIAzoland miRNeasy Micro Kit combined with recommended RNase-free DNase I treatment (all from Qiagen, Hilden, Germany). The quality of extracted RNA was assessed using Bioanalyzer and RNA 6000 Nano Kit (Agilent) ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were lysed using 1 mg/mL lysozyme dissolved in TE buffer and purified using RNeasy Mini Kit with on-column DNA digestion with DNase I (Qiagen, Germantown, MD) following manufacturers’ instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
-
bioRxiv - Plant Biology 2023Quote: ... was treated with RNase-free DNase I to remove residual genomic DNA and cleaned using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... DNA removal was performed by applying 80 μl of DNase I working solution (10 μL DNase stock + 70 μL 1x RDD buffer; RNase-Free DNase Set, Qiagen, Hilden, Germany) to the column and incubated for 15 min at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: DNA was extracted from each sample type using three different approaches: 1) Qiagen (QIAamp® DNA minikit or QIAamp® DNA Stool Mini Kit, Qiagen Inc., Germantown, MD, USA); 2 ...
-
bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
-
bioRxiv - Microbiology 2023Quote: ... Viral DNA was purified and extracted from 100 μL of DNase I-treated culture supernatant using the QIAamp DNA blood mini kit (QIAGEN GmbH, Hilden, Germany). To quantify viral DNA copies ...