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Citations for Qiagen :
451 - 500 of 533 citations for Penicillin G since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... were transferred into 0.2-mL PCR tubes and genome amplified using REPLI-g Advanced DNA Single Cell Kit (Qiagen Hilden, Germany). For the whole culture approach ...
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bioRxiv - Microbiology 2020Quote: ... bacterial cultures were centrifuged for 10 minutes at 4,300 x g and the bacterial cell pellet resuspended in the lysis buffer (RLT) from the RNeasy kit (Qiagen, Germantown, MD). Bacterial cells were then homogenized with sterile 0.1 mm glass beads (Biospec Products Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... Lysates were clarified by centrifugation at 30,000 g for 30 min and loaded on a Ni-NTA Superflow column (Qiagen, Netherlands, 30761). After washing with each 20 column volumes of lysis and wash buffer (50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: DNA extraction was performed by mixing 0.2 g of each feces sample with 500 μL of G2 buffer (QIAGEN, Hilden, Germany) in an Eppendorf tube (Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Frozen muscle was pulverized in liquid nitrogen and genomic DNA was extracted using a Genomic Tip 100/G kit (Qiagen, USA). DNA quantity was measured using a NanoDrop ND-8000 spectrophotometer and a Qubit dsDNA BR Assay kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μg of RNA per sample was reverse transcribed using a complementary DNA (cDNA) synthesis kit (QuantiTect reverse transcription kit, Qiagen, Germany). Quantitative PCR (qPCR ...
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bioRxiv - Genomics 2022Quote: ... We extracted genomic DNA with standard CTAB method and purified it with QIAGEN Genomic-tip 20/g (Qiagen KK, Tokyo, Japan).
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bioRxiv - Genetics 2022Quote: ... The nuclei were sorted into individual tubes and subject to single nucleus whole genome amplification (snWGA) using the REPLI-g® UltraFast Mini kit (Qiagen). The snWGA was performed by a multiple displacement amplification as in this previous publication (85) ...
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bioRxiv - Microbiology 2023Quote: Samples were thawed on ice and 6-10 aliquots of approximately 0.25 g each were subjected to DNA extraction using the PowerLyzer® PowerSoil® DNA Isolation kit (QIAGEN) following the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2023Quote: DNA from ten (~0.25 g each) pieces of store-bought chicken breast was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2023Quote: A portion of the total cvcDNA preparation was subjected to rolling circle amplification (RCA) by using a Repli-G mini kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysate was clarified by centrifugation at 30,000 ×g for 30 min and the supernatant was loaded onto a column packed with Ni-NTA agarose (Qiagen, Hilden, Germany). The column was sequentially washed with buffer I (40 mM Tris-HCl ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from ∼0.25 g of sponge tissue in a laminar flow hood using the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) using the optimized procedure of Marotz et al ...
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bioRxiv - Microbiology 2023Quote: ... and FH samples containing leaf and root fragments were macerated in liquid nitrogen and 0.25 g of the macerated material was used for DNA extraction with the DNeasy Power Soil kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The capsules were then suspended in Buffer D2 and subjected to multiple displacement amplification (MDA) using REPLI-g Single Cell Kit (QIAGEN, Germany). Following MDA treatment at 30°C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial genomic DNA was isolated from pure culture using QIAGEN Genomic-tip 100/G following the manufacturer’s instruction of (Qiagen, Valencia, CA). The Seqwell plexWell LP384 Library Preparation Kit and Native Barcoding Kit 24 V14 (SQK-NBD112.24 ...
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bioRxiv - Systems Biology 2023Quote: ... The spheroblasted cells were collected in 50 mL Falcon tubes and spun for 5 min at 2,500 g and re-suspended in 7.5 mL of home-made buffer P1 (according to the manufacturers recipe, QIAGEN, Düren, Germany). 7.5 mL of home-made buffer P2 (according to the manufacturers recipe ...
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bioRxiv - Cancer Biology 2024Quote: PC9 and CRUK0764 cells were immediately centrifuged at 300 × g for 4 min to remove supernatant and add 350 µl RLT buffer (Qiagen, 79216) containing 1% β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from each 2 g of bulk and rhizosphere soils of the pepper plants using a RNeasy PowerSoil Total RNA Kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... were centrifuged at 4,000 × g for 15 min and viral RNA was extracted from each supernatant using the QIAamp Viral RNA minikit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... injected embryos developed to blastocyst stage in culture were collected in 1.5 ul water individually and the whole genome was replicated using a REPLI-g® Mini Kit (Qiagen, Germantown, MD) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: DNA extractions were performed using ∼10 g of sediment with the PowerMax Soil DNA Isolation Kit (MO BIO Laboratories, Inc.; Qiagen, Hilden, Germany). To increase the DNA yield ...
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bioRxiv - Neuroscience 2020Quote: ... 500 GFP-positive and 500 GFP-negative nuclei were sorted directly into 3uL of REPLI-g Advanced Storage buffer (Qiagen Cat#: 150365) in a PCR tube strip (Genessee Cat# ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of strains IMX2300 and IMX2300-1 was isolated with a Blood & Cell Culture DNA Kit with 100/G Genomics-tips (QIAGEN, Hilden, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Microbiology 2020Quote: ... single Achromatium cells were obtained as previously described (Ionescu et al. 2017) and amplified using the Repli-G kit (Qiagen, Hilden, Germany) following the manufacturer instructions ...
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bioRxiv - Zoology 2021Quote: ... High-molecular-weight genomic DNA was prepared from the kidney of one male shrew using a Genomic-tip 20/G (QIAGEN, Germantown, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... High molecular weight DNA was extracted from 40mg frozen liver from both species using Qiagen Genomic Tip 100/G kit (Qiagen, Hilden, Germany). DNA integrity was assessed visually by separating fragments on a 0.5% TAE agarose gel which revealed a predominant band of high molecular weight DNA >45kb ...
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bioRxiv - Genetics 2022Quote: ... and extracted and amplified from vitrified blastocysts by thawing in phosphate-buffered saline supplemented with 5% bovine serum albumin and processing with REPLI-g whole genome amplification methods (Qiagen, Cat. #150343). Informed consent was obtained from all participants (custodians of the blastocysts ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was extracted from the blood (N. scutatus) or tail clip (P. textilis) using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with proteinase K (NEB ...
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bioRxiv - Microbiology 2021Quote: ... total DNAs from these samples (20 g each) were extracted using the Qiagen DNeasy® PowerSoil® Pro Kit (Qiagen, Hilden, Germany) and the integrity of DNA was evaluated by gel electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pelleted at 500 g for 5 min and total RNA was extracted using RNeasy Mini Kit (QIAgen, Cat No. 74104), including DNAse digestion step (QIAgen ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from pelleted nuclei with the Qiagen Genomic tips 20/G and HMW DNA extraction buffer set (Qiagen no. 19060) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 1µg/ml Aprotinin, 1mM PMSF) using 5mm Stainless Beads and TissueLyser II (frequency: 30/sec, duration 2 × 30 sec) (Qiagen, Hilden, Germany). IL-23 was measured using either a mouse IL-23 ELISA (R&D ...
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bioRxiv - Microbiology 2020Quote: ... Two PowerBead preparations of 0.5 g were used along with the PowerSoil Pro DNA Isolation kit (MoBio Labs/QIAGEN, Carlsbad, CA, USA) according to the instructions ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Developmental Biology 2021Quote: ... from entire blastocysts (sibling day-8) was whole-genome amplified by multiple displacement amplification (MDA) with a REPLI-g Single Cell Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions with full or half reaction volumes for the fast 3-h protocol ...
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bioRxiv - Microbiology 2021Quote: Soil genomic DNA was extracted from 0.25 g of thawed soil rhizosphere and bulk samples using the DNeasy Power Soil Kit (Qiagen, Germantown, MD, USA). Amount and purity of DNA were assessed using a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... The total genomic DNA was extracted from 0.25 g of soil using the Power Soil DNA Isolation Kit (Qiagen, Carlsbad, CA, USA) in the Microbial Ecology and Biotechnology Laboratory (LEMBiotec ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Genomics 2021Quote: A half-loopful overnight grown bacterial culture was used to extract the genomic DNA using the QIAGEN Genomic-tip 100/G (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... A total of 44 single-sperm samples were selected to undergo whole genome amplification (WGA) with the REPLI-g Single Cell Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... total DNA extraction was performed on this suspension of freshly grown bacterial colonies using the Genomic-tip 500/G kit (Qiagen, Hilden, Germany). Plasmids were electrophoresed in a 0.5% SeaKem Gold agarose with a Tris-acetate buffer ...
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bioRxiv - Microbiology 2022Quote: ... 2 g of soil was immediately transferred into pre-weighed sterile tubes containing 3 ml of LifeGuard soil preservation solution (Qiagen, Toronto, CA) to stabilize the RNA ...
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bioRxiv - Neuroscience 2022Quote: Total forebrain RNA (10 μg) was isolated from three pairs of littermate matched AtrxFoxG1Cre and control P17 mice using the RNeasy Mini kit (Qiagen Cat# 74104). cRNA was generated and hybridized to an Affymetrix Mouse Genome 430 2.0 Array at the London Regional Genomics Center (London ...
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were thawed and DNA was extracted using the phenol-chloroform protocol detailed above without the liquid nitrogen grinding step (CCS156, CCS165, CCS166 and CCS176) or using the Qiagen Genomic-tip 20/g kit (Qiagen, Venlo, Netherlands) according to manufacturer instruction ...
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bioRxiv - Microbiology 2022Quote: The resulting lysate was then loaded onto a Qiagen Genomic-tip 20/G column and purification was performed following manufacturers recommendations (Qiagen, Hildern, Germany). The Genomic-tip 20/G column was equilibrated with 1 mL of Buffer QBT equilibration buffer (750 mM NaCl ...
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bioRxiv - Genomics 2023Quote: Genomic DNA for the Pacific Bioscience Single Molecule Real-Time (SMRT) sequencing was prepared from 2 mL of fresh blood using the genomic-tip 100/G kit (Qiagen, Hilden, Germany). This was performed with additional RNase (Astral Scientific ...
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bioRxiv - Microbiology 2023Quote: DNA extraction from 0.25 g rhizosphere soil (frozen at −80°C) was performed using the DNeasy PowerSoil Pro kit (QIAGEN GmbH, Hilden, Germany) according to manual ...