Labshake search
Citations for Qiagen :
1 - 50 of 3324 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
-
bioRxiv - Microbiology 2023Quote: ... subtilis 3610 genome (CP020102) (98) using CLC Genomics Workbench software (Qiagen). The enrichment at ribosomal RNA locations were eliminated and the number of reads mapped to each base pair in the genome was exported into a .csv file ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μg/mL RNase A (Qiagen PI buffer). Bacteria were then lysed by adding 300 μL of buffer containing 200 mM NaOH ...
-
bioRxiv - Biochemistry 2023Quote: ... 100 μg/mL RNase A (Qiagen PI buffer). Bacteria were then lysed by adding 300 μL of buffer containing 200 mM NaOH ...
-
bioRxiv - Microbiology 2020Quote: ... it was diluted in 50 μL of AE buffer (Qiagen, Valencia, CA). Prior the library preparation ...
-
bioRxiv - Developmental Biology 2024Quote: ... We used barcoded adaptors for indexing the libraries (Qiagen, 98 TruSeq v2 kit barcoded adaptors). We sequenced single-ended 50 bp strategy using a HiSeq Illumina 2500.
-
bioRxiv - Cancer Biology 2024Quote: ... The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 μg/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 μg/mL ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
-
bioRxiv - Physiology 2020Quote: ... 0.1% SDS and 50 mM Tris) in a tissue lyser (TissueLyser II, Qiagen, Vancouver, CA) then sonicated for 10 minutes in a Q500 sonicator (QSonica ...
-
bioRxiv - Cancer Biology 2024Quote: ... Viable (PI-) single cells were sorted into 2.5µL of RLT PLUS buffer (Qiagen) containing 2.5U SUPERas-In (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... and diluted 1:50 in TE-Buffer (Qiagen, #1018499) to a final concentration of 1.75e12 vg/ml (kindly donated by I ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA from liver tissues (50-100 mg) were prepared using Qiagen RNeasy kit (Qiagen, Valencia, CA) according to the manufacture’s protocol and cDNA was synthesized as published [24] ...
-
bioRxiv - Neuroscience 2023Quote: ... FACS-sorted cells (≈ 98% tdTomato+ cells) were harvested in RLT and RNA extracted with RNeasy Micro kit (Cat. # Qiagen). Around 100 000 tdTomato+ cells were sorted from 10 electroporated-brains for each sample to sequence ...
-
bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted by incubation with 6 ml of lysis buffer (50 mM Tris pH 8.0, 50 mM EDTA, 1% SDS, 0.1 mg/ml proteinase K (Qiagen, 19131)) at 55°C for 16 hours ...
-
bioRxiv - Bioengineering 2024Quote: ... 1 mL of 50% Ni-NTA agarose (Qiagen, Toronto, Ontario, Canada) was equilibrated using 10 bed volumes of wash buffer (same formulation as lysis buffer) ...
-
bioRxiv - Genetics 2020Quote: ... suspended in 1 mL of (v/v) 50% ACN and homogenized in a bead mill (50 Hz, 5 min; TissueLyser LT, Qiagen, Germany) using two 5 mm tungsten balls ...
-
bioRxiv - Microbiology 2022Quote: ... each sample was eluted in 40.5 µL elution buffer that was prepared by mixing 98 µL Buffer EB (Qiagen, CN 19086), 1 µL 10% Tween 20 (Teknova ...
-
bioRxiv - Neuroscience 2023Quote: ... samples obtained by FACS together with 100 ng carrier RNA (PolyA- and PolyA+ mixed in 98:2 ratio) were lysed by Buffer RLT Plus (Qiagen, USA). Then we used PolyA mRNA Magnetic Isolation Module (NEBNext ...
-
bioRxiv - Cell Biology 2020Quote: ... was carried out after treatment with 50 μg ml−1 RNase (Qiagen) for 20 min at 37°C ...
-
bioRxiv - Plant Biology 2021Quote: ... were set up with a CAS-1200N robotic liquid handling system (Corbett Research) and run for 50 cycles on a Rotor-Gene Q (Qiagen). Two technical replicates and three biological replicates were performed for each sample ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 µL of RNase inhibitor and 98 µL nuclease-free water) and the eluted RNA was then concentrated using the RNeasy MinElute Cleanup kit (Qiagen, ref. 74204). The immunoprecipitated RNA was then reverse transcribed and used for qPCR ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... or Junctional Adhesion Molecule 1 (JAM1) using Polyfect (Qiagen, CA, USA) and selected in 1000 µg/ml G418 for stable transfectants ...
-
bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated from 50 to 300 µl nematode pellets using the Blood and Tissue DNA isolation kit (cat# 69506, QIAGEN, Valencia, CA) following established protocols (Cook et al ...
-
bioRxiv - Neuroscience 2024Quote: Microbial sequencing was performed as previously described in.21,22 Microbial DNA was extracted from 50∼100 mg fecal material using the Qiagen DNeasy PowerSoil Pro Kit and the automated QIAcube Connect robot (Qiagen, Carlsbad CA) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: NHEKs were transfected at 50-70 % confluency with 1 µL HiPerfect transfection reagent (Qiagen) and 5 nM of either AhR-specific “SilencerSelect” siRNA (s1199 ...
-
bioRxiv - Microbiology 2020Quote: ... were homogenized 7 min at 50 pulses s-1 with the Tissuelyser LT (QIAGEN) prior to incubation in lysis buffer for 1 h at 60°C for increasing cell lysis ...
-
bioRxiv - Cancer Biology 2022Quote: ... 50 mg tumor tissue was mechanically homogenized in 1 ml Qiazol (Qiagen, Hilden, Germany), whereas cells were lysed in 1 ml Qiazol by resuspending ...
-
bioRxiv - Microbiology 2023Quote: TG was quantified in snap-frozen liver tissue stored at −80 □C until cryo-grinding in liquid nitrogen and 50 ±5 mg tissue added 0.9 mL of a 2:1 chloroform:methanol solution and homogenized 1 min at 50 os/sec using a TissueLyser LT (Qiagen) with beads ...
-
bioRxiv - Microbiology 2021Quote: ... qPCR reactions contained 4 μl of cDNA (1:50 dilution) and 200 nM of each primer (supplementary table 1) and QuantiTect SYBR Green PCR master mix (Qiagen) or SensiFAST SYBR Hi-ROX kit (Bioline) ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by three rounds of washes with sterile PBS + PI for 45 mins, then subjected to one round of incubation with DNase I (4716728001, MilliporeSigma) + RNase A (19101, Qiagen) + PI for 72 hours ...
-
bioRxiv - Genetics 2024Quote: Total RNA was isolated from the center of the second leaf of Pst-non-inoculated PI 487260 seedling at two-leave stage using the RNeasy plant mini kit (Qiagen). A non-size-selected PacBio Iso-Seq library was prepared and sequenced on a Sequel II system (PacBio ...
-
bioRxiv - Genomics 2020Quote: ... Total RNA was extracted from 200 mg of ground roots and 50 mg of ground leaves or culms using an RNeasy Plant Mini Kit (Qiagen, Valencia, CA, United States). We quantified the RNA and verified its integrity in a 2100 BioAnalyzer using a Eukaryote Total RNA Nano Assay (Agilent Technologies) ...
-
bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
-
bioRxiv - Microbiology 2020Quote: Total RNA extraction was performed using a squash buffer (10 mM Tris base, 1 mM EDTA, 50 mM NaCl) supplemented with 1:8 part Proteinase K (Qiagen, 15mg/ml). Mosquito abdomens with the midguts and heads with thoraces were individually collected in 50 μl of squash buffer at 7 and 14 days ...
-
The mitochondrial genome of the red icefish (Channichthys rugosus) casts doubt on its species statusbioRxiv - Zoology 2022Quote: ... 50 μL Buffer AE (Qiagen) were placed directly onto the silica membrane ...
-
bioRxiv - Developmental Biology 2023Quote: ... 50 μg/ml DNase (Qiagen) and 100 μg/ml heparin (Sigma) ...
-
bioRxiv - Cell Biology 2024Quote: RNA was isolated from RPE-1 cells using the RNeasy mini kit (Qiagen, CA) and cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... along with 8 prickleless parental accession individuals (S. macrocarpon PI 441914) was extracted from young leaf tissue using a DNeasy Plant Pro Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions for high-polysaccharide content plant tissue ...
-
bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was mixed with 1 ml of 50% Ni2+-NTA His-bind resin (Qiagen, Hilden, Germany), incubated for 1 hr at 4°C with mild rotatory shaking ...
-
bioRxiv - Developmental Biology 2022Quote: ... cells were transfected with 500ng plasmid DNA and a 1:50 Effectene transfection reagent ratio (Qiagen #301425).
-
bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
-
bioRxiv - Plant Biology 2020Quote: ... MCD360-1 and the F1 progenies using the DNeasy Plant Mini Kit (Qiagen, Valencia, CA). Equal amounts of DNA were pooled from 30 responsive as well as 30 non-responsive progenies for the INF1 recognition phenotype and 29 responsive and 30 non-responsive individuals for the SCR74 response phenotype ...
-
bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
-
bioRxiv - Immunology 2023Quote: ... 25 nM and 50 nM of synthetic hsa-miR-30b or control mimics (50 nM) (Qiagen). At 36 h post-transfection ...
-
bioRxiv - Cancer Biology 2020Quote: ... were purchased from Qiagen (Qiagen, Valencia, CA) and qPCR was performed using the Qiagen miSCRIPT SYBR® Green PCR kit (Qiagen ...
-
bioRxiv - Microbiology 2022Quote: ... 50 μl proteinase K solution (Qiagen), and 50 μl 1M dithiothreitol (DTT) ...
-
bioRxiv - Biochemistry 2020Quote: ... 50 μl Ni-NTA agarose (Qiagen) and 50 μl Neutravidin resin (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... GapmeRs (Qiagen, Hilden, Germany; 50 nM) were transfected using Lipofectamine 2000 (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...