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Citations for Qiagen :
1 - 50 of 2287 citations for PEGSSDA 2 x 0.5 mL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% Triton X-100) with 50 µl protinase K (>600 mAU/ml, Qiagen) and incubated at 37°C for 30min ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.5% (v/v) Triton X-100) and 1 mg/ml Proteinase K (QIAGEN, Germany). The solution was incubated at 55°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Synthetic Biology 2021Quote: ... ∼2.5×108 cells (0.5 ml of OD600 0.5) were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and pelleted ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 mM of NaCl and 2 μL of RNase A (0.5 mg / mL) (Qiagen) were added to the collective eluates which were incubated at 65 °C overnight ...
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bioRxiv - Physiology 2022Quote: ... approximately 100 mg of adipose tissue and a 7 mm metal bead were placed in a 2 mL centrifuge tube containing 0.5 mL PBS buffer (pH 7.4) and homogenised in TissueLyser II (Qiagen) for 2 minutes at 25 Hz ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in lysis buffer (DPBS with 0.5% Triton X-100, 10 mM MgCl2, 5 mM CaCl2, 100 µg/mL RNAse A (Qiagen)) overnight at 37°C in a water bath to allow capsid maturation ...
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bioRxiv - Physiology 2020Quote: ... Groups of five flies were transferred to 2 mL tubes and were homogenized in 100μl PBS + 0.1% Triton X-100 using a TissueLyser LT (Qiagen). The samples were then spun down at 12,000 rpm and the supernatant transferred to fresh vials and the absorbance of each sample at 603 nm was measured using a NanoDrop ...
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bioRxiv - Microbiology 2024Quote: ... followed by 0.5 mg/mL proteinase K (Qiagen) incubation at 56°C for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of Ni-NTA agarose resin (Qiagen, 0.5 mL bed volume) was equilibrated in lysis buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... ~5×108 cells (1 ml of OD600 0.5) were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and pelleted ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 10 x 1 cm column with 2 mL Ni-NTA Superflow resin (Cat. #30230, Qiagen) that was pre-equilibrated with Buffer A (10 mM CAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... and finally 0.5 mL of Ni-NTA agarose (Qiagen) that had been washed 3 times with lysis buffer containing 20 mM imidazole ...
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bioRxiv - Immunology 2023Quote: ... immersed in 0.5 mL of RNA-later (Qiagen, Hilden, Germany) and stored at -80°C ...
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bioRxiv - Plant Biology 2020Quote: ... and finally eluted in 2 x 30 μL EB Buffer (Qiagen) at 60°C.
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 0.5 ml RNA Later (Qiagen, Germany), stored at 4°C overnight and then transferred to −80°C until time for RNA extraction ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant was applied to 0.5-1 mL Ni-NTA resin (Qiagen) in gravity columns pre-equilibrated in lysis buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 mL of cells were harvested with RNAprotect Bacteria Reagent (Qiagen) 3-4 h after induction ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM TCEP) containing RNase A (0.1 mg/ml, Qiagen), lysed by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM TCEP) containing RNase A (0.1 mg/ml, Qiagen), lysed by sonication ...
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bioRxiv - Molecular Biology 2024Quote: ... The clarified lysate was incubated with 0.5 mL NiNTA resin (QIAGEN) for 1 hr at 4°C on a rotary ...
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bioRxiv - Neuroscience 2020Quote: ... lumbar spinal cord or the glabrous skin of the footpad were harvested and homogenized in 0.1M HCl + 0.5% triton X-100 using glass beads and a TissueLyser II (Qiagen). Lysate was centrifuged at 18,000 xg and 4 °C for 40 – 60 min to pellet cell debris ...
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bioRxiv - Biophysics 2022Quote: ... were filled with ( 1 mL | 0.5 mL ) of bed Ni-NTA resin (Qiagen or Thermo Fisher), and equilibrated with three rinses of Wash buffer (at least 30 resin bed volumes ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of each culture was mixed with 1 mL of RNA Bacteria Protect Reagent (Qiagen) immediately after 2 mM ZnCl2 induction (t0 ...
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bioRxiv - Microbiology 2019Quote: ... containing 2 ml microcentrifuge tubes (QIAGEN) in which samples were placed ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg/mL RNase A (QIAgen), 1/200 (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mL Ni-NTA resin (Qiagen) was used for clarified cell lysate from 1 L bacterial culture ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 ml of culture was mixed with 1ml of RNA protect (Qiagen) immediately prior to metal addition (t0 ...
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bioRxiv - Plant Biology 2020Quote: ... The extracted DNA was eluted in 2 x 100 μL EB buffer (Qiagen), cleaned and concentrated with the Clean and Concentrator-5 kit (Zymo Research ...
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bioRxiv - Genetics 2021Quote: ... ThermoScientific catalog numbers A32955 and 78420)) using a bead mill with steel beads (2 x 2 min at 20 Hz; TissueLyser, Qiagen). Extracts were cleared by centrifugation (17,000 RCF ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 ml of the non-stressed culture was added to 2 ml of the RNAprotect Bacteria Reagent (Qiagen), vortexed and incubated for 5 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Nhp6 was pulled down with 0.5 ml packed Ni-NTA agarose beads (Qiagen) for 3 hours at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was filtered and loaded on two 0.5 ml Ni-NTA (Qiagen) columns (one column/L growth ...
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bioRxiv - Microbiology 2022Quote: RNA was isolated from 2 x 106 cells using RNeasy plus mini Kit (Qiagen). Reverse transcription was performed with either random decamers or HIV antisense-specific primers ...
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bioRxiv - Pathology 2022Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 3.2 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 30 r/s for 3 cycles ...
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bioRxiv - Pathology 2023Quote: ... with 2 x 5 mm stainless steel beads using a TissueLyser (Qiagen, Germantown, MD) for 3 minutes at 25 r/s for 2 cycles ...
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bioRxiv - Biophysics 2021Quote: ... incubated with 2 ml nickel resin (Qiagen) and washed with 40 mM imidazole in 30 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... incubated with 2 ml nickel resin (Qiagen) and washed with 40 bead volumes of 40 mM imidazole in 30 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 x 2 minute intervals at 25 Hz with the addition of one 7 mm stainless steel bead (Qiagen #69990). 200 uL of tissue lysate was used for KingFisher Flex (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cells suspended in lysis buffer were transferred to a 2 mL screw cap tube that contained 0.5 mL of 0.1 mm disruption beads for bacteria and 0.7 mL QIAzol™ Lysis Reagent (Qiagen; 79306). Cells were lysed via mechanical disruption using a bead beater for four rounds of 30 s each ...
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bioRxiv - Genetics 2022Quote: ... RNA was collected from 2 x 105 pelleted cells using the RNeasy mini kit (Qiagen). qPCR using primers specific for ERAP1 or TNFSF12 were used to validate knockdown relative to 18S rRNA.
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from 2 x 106 cells with the RNeasy Plus Mini kit (Qiagen) and reversely transcribed with SuperScript III (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from 1 – 2 x 108 cells using the RNeasy kit (Qiagen). DNA was removed using Turbo DNase (Applichem ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was gently shaken with 0.5 ml of Ni-NTA agarose suspension (Qiagen) for 1 h at 4 °C and transferred to a filter column where the liquid was drained ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was added to 0.5 mL (bed volume) Ni-NTA resin (Qiagen) equilibrated in wash/lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was incubated with Ni-NTA agarose beads (0.5 ml; Qiagen) for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots (0.5 ml) of macerated xylem chips in PowerBead tubes (DNeasy PowerSoil Kit, QIAGEN) were homogenized 7 min at 50 pulses s-1 with the Tissuelyser LT (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 ml of culture supernatant was incubated with nickel-nitrilotriacetic acid-agarose beads (QIAGEN). The beads were washed ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then mixed with 0.5 mL of Ni-NTA Agarose resin (Qiagen), equilibrated with 50 mM NaH2PO4 ...