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Citations for Qiagen :
551 - 600 of 1879 citations for P N Nonylphenol Diethoxylate Unlabeled 500 Ug Ml In Acetonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells suspended in lysis buffer were transferred to a 2 mL screw cap tube that contained 0.5 mL of 0.1 mm disruption beads for bacteria and 0.7 mL QIAzol™ Lysis Reagent (Qiagen; 79306). Cells were lysed via mechanical disruption using a bead beater for four rounds of 30 s each ...
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bioRxiv - Systems Biology 2023Quote: ... A culture volume equal to 3 mL of OD = 1 was added to 6 mL RNAprotect Bacteria Reagent (Qiagen), vortexed ...
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bioRxiv - Cell Biology 2020Quote: ... RNA (500 ng) was reverse transcribed to produce cDNA libraries using the QuantiTect Reverse Transcriptase Kit from Qiagen (205311). cDNA was quantified by Qubit analysis with the 1× dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of each native library were treated by sodium bisulfite using the EpiTect Bisulfite kit (Qiagen, Valencia, CA) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA (500 ng) was extracted from overnight cultures using the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) and mechanically fragmented for 40 s using a Covaris M220 (Covaris ...
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bioRxiv - Cell Biology 2021Quote: ... 200 to 500 ng of RNA were used to prepare cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, 205311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... when 500 µl of recipient culture was incubated with 2.4 µg of VFS donor DNA (prepared with the Qiagen Blood and Tissue kit ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of total RNA was used for establishing the cDNA library with the miScript II RT Kit (Qiagen). qRT-PCR was performed with the SYBR Green I Master kit (Roche Applied Science ...
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bioRxiv - Immunology 2022Quote: Bacterial genomic DNA was isolated using the Blood and Cell Culture DNA kit and 500/G genomic tips (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR products of the expected ∼500 bp were purified from the gel using a QIAquick Gel Extraction kit (Qiagen) and sequenced in both directions by Eurofins Genomics (Toronto ...
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bioRxiv - Genetics 2019Quote: ... 500 mg of whole flies were then used for DNA extraction with Blood Cell and Culture Midi Kit (Qiagen) (Chakraborty et al ...
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bioRxiv - Physiology 2021Quote: ... The hemolymph was diluted in 500 μL 90% (v/v) methanol, homogenized using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Genetics 2020Quote: ... DamID was performed on 500 ng gDNA extracted from the animals using DNeasy Blood and Tissue Kit (Qiagen #69504). Two replicates for each stage and cell type were processed ...
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bioRxiv - Physiology 2020Quote: ... then washed with PBS and crushed in 500 μL of LB medium with a Tissue Lyser (Qiagen ref. 85600) at 50Hz for 5 minutes ...
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bioRxiv - Immunology 2021Quote: Liver tissue (50-70 mg) was homogenized in 500 μl of 0.1 % NP-40 in PBS using a bead mill Tissue Lyser (Qiagen) and centrifuged for 2 min at 3000 x g to remove the insoluble material ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was extracted from a pool of 500 MN per mouse with the microRNeasy kit according to manufacturer’s instruction (Qiagen). RNA was quantified with a Qubit (fluorimeter from Life Technologies ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from the sediment samples (∼500 mg wet weight) with the PowerSoil DNA bead tubes (Qiagen, USA), using the FastPrep-24™ Classic (MP Biomedicals ...
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bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and RNA was isolated using the RNeasy kit (Qiagen), as instructed by the manufacturer’s manual ...
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bioRxiv - Microbiology 2022Quote: ... homogenized in 500 μl PBS by bead beating (3 mm steel ball, 25 Hz for 2.5 min in a TissueLyser (Qiagen)) ...
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bioRxiv - Immunology 2023Quote: ... and plasmid DNA from 200-500 µl of the cell suspension was purified using QIAprep Spin Miniprep Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and PCR products were purified and concentrated to 500 ng/uL using a MinElute PCR product purification kit (Qiagen). Strains containing pKD46 were grown at 30 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 50 mg of tissue was homogenized in 500 µl Sucrose Buffer (250 mM sucrose, 20 mM imidazole, pH 7.0) using a TissueLyser II (Qiagen). Tissues from 3-4 mice were pooled together ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed once with PBS to remove residual media and then lysed with 500 µL RLT buffer (Qiagen) with 5 µL of β-mercaptoethanol (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... A total of 500 ng of RNA was used to synthesize cDNA using the QuantiTech reverse transcription kit (Qiagen). The cDNA samples were then diluted 1:10 and used as a template to perform RT-qPCR ...
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bioRxiv - Immunology 2019Quote: ... longitudinal muscle/myenteric plexus preparations were homogenized in a 2 ml eppendorf containing 1 ml Trizol and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Immunology 2019Quote: ... mucosal scrapings and feces were homogenized in a 2 ml eppendorf containing 1 ml of DNA extraction buffer and a single 5 mm steel bead in a TissueLyzer (Qiagen) for 3 min at 30 Hz ...
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bioRxiv - Microbiology 2021Quote: ... 1 g yeast extract in 750 ml of 30 kDa filtered seawater and 250 ml of Milli-Q water) using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
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bioRxiv - Microbiology 2020Quote: ... and the cycle-threshold CT translated to bacterial load (estimated CFU per mL (eCFU/mL) using a standard curve on a Rotor gene Q 5plex platform (Qiagen). The cut-off for TB-MBLA positivity is a 30 CT value that corresponds to 1.0 log10 eCFU/mL ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Transcripts were stabilized by mixing 3 mL of cell cultures at the mid-log phase with 6 mL of RNAprotect Bacteria Reagent (Qiagen). Samples were immediately vortexed for 5 sec ...
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bioRxiv - Microbiology 2022Quote: ... from the top A horizon were immediately collected for DNA extractions in 15 mL tubes containing 5 mL Lifeguard Soil Preservation Solution (QIAGEN) using chilled soil-processing trays ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was transferred to a 15 ml falcon tube and 1 ml of a 50% slurry consisting of NiNTA agarose beads (Qiagen) in resuspension buffer was added per 10 ml of lysate ...
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bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
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bioRxiv - Microbiology 2021Quote: ... Mosquito bodies and legs and wings were put into a 2 ml round-bottom tubes containing 250 ml of PBS and a steel ball (Qiagen). Samples were homogenized using a TissueLyser (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... in 96 flat bottom plates in the presence or absence of PMA (200ng/ml) and ionomycin (300ng/ml) were lysed in 100Sµl lysis buffer containing TCL buffer (Qiagen, 1031576) containing 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Biochemistry 2021Quote: ... and early stationary phase (OD = 1.0-1.3).1 mL sample of each culture was directly transferred to 2 mL RNAprotect cell reagent (Qiagen, Hilden, Germany). The samples were vortexed 5 sec ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with ammonium sulfate as in Niu et al.49.The precipitate was dissolved in 30 ml buffer C and loaded onto a 5 ml column of Ni-NTA-agarose (Qiagen) pre-equilibrated in buffer C ...
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bioRxiv - Molecular Biology 2023Quote: ... fungal DNA was extracted independently from 1 mL of crude conidial suspensions (between 108 and 109 spores mL-1) using the DNeasy 96 Plant Kit (Qiagen) following the manufacturer’s instructions (Gautier et al. ...
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bioRxiv - Microbiology 2023Quote: ... Pellets were washed in 2 mL cold 10 mM NaCl + 4 mL cold RNAprotect Bacteria Reagent (Qiagen Cat. No. 76506) and repelletted at 4,255 x g ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with 0.1 mg/mL RNaseA (Qiagen) for 2h at 37°C ...
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bioRxiv - Genomics 2020Quote: ... using 10X (2.5 ml) of PB buffer (Qiagen) following the manufacturers’ instructions with the only change being a 10 minute incubation at 37 degrees prior to the final elution spin and eluted in 100 μl of EB buffer (QIAGEN) ...
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bioRxiv - Microbiology 2019Quote: ... 1 ml RLT buffer (Qiagen RNA Isolation Kit) + 10 μl β-mercaptoethanol was added and vortexed and a phenol chloroform extraction followed by an ethanol precipitation was carried out ...
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bioRxiv - Cancer Biology 2020Quote: ... and 100 μg/mL of RNase A (Qiagen). Cell death was assessed using flow cytometric analysis of Annexin V and propidium iodide staining according to the manufacturer’s instructions (eBioscience) ...
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bioRxiv - Biochemistry 2020Quote: ... 3-4 mL of Ni-NTA resins (Qiagen) were applied on the gravity column ...
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bioRxiv - Plant Biology 2021Quote: ... followed by proteinase K treatment (200ug/ml; Qiagen) at 50°C overnight ...