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Citations for Qiagen :
601 - 650 of 10000+ citations for Oxytocin Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The template was run on a 1% agarose gel and extracted using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). DNA template concentration was determined by a Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from Achilles enthesis of 1-dpi pups or from tissue culture cells using the RNeasy Kit (Qiagen). Reverse transcription was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... protocol 1 in the RNAprotect Bacteria Reagent Handbook (Enzymatic lysis of bacteria) was followed using the RNeasy Plus Mini Kit (Qiagen) using TE buffer (10 mM Tris-HCl ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from individual pools of 1×104 to 2×104 double-sorted SLAM cells using an RNEasy Micro kit (Qiagen). RNA was quantified and quality checked using an Agilent Bioanalyzer 2100 (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... and 18 centimeters below seafloor (cmbsf) from station AC of Van Keulenfjorden (Supp. Table 1) using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... ~100 mg tissue was homogenized in 1 mL Qiazol and RNA was extracted using RNeasy Lipid Tissue Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was isolated from a suspension of 1 x 106 PBMCs from each individual in the study using a DNA isolation kit (Qiagen). Genotyping was then performed using the Infinium CoreExome-24 (v1.3 ...
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bioRxiv - Genetics 2019Quote: ... DNA was size fractionated on 1% agarose gels and 500-750 bp DNA fragments were gel extracted with the Qiaquick gel extraction kit (Qiagen). A first round of PCR was performed using primers GSP1 and P1 (First denaturation step ...
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bioRxiv - Genetics 2019Quote: ... for the PCR analysis of potential recombination events genomic DNA from 1×10^6 cells was extracted using DNeasy Blood & Tissue Kits (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
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bioRxiv - Cancer Biology 2020Quote: ... or 50 um sections (liver metastases) were cut and homogenized in 1 ml of AllPrep DNA/RNA Mini Kit (Qiagen) using a plastic pestle (Cole-Palmer #44468-23) ...
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bioRxiv - Bioengineering 2022Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto pools using the QIAsep 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation before sequencing on an Illumina MiSeq platform at the Duchossois Family Institute Microbiome Metagenomics Facility at the University of Chicago ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified by ethanol precipitation and 1% agarose gel purified using the QIAquick Gel Extraction Kit (Qiagen). Single-round E ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... At day 28 ASOs were added (final concentration of 0.2, 1 or 7.5 μM) and RNA was harvested (RNeasy mini kit [Qiagen, Cat#: 74106]) after 5 days of treatment ...
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bioRxiv - Genomics 2022Quote: ... RNA (3 replicates each of V or E2 treated samples from donor 1 and donor 2) was DNAse treated and cleaned up using the RNeasy Mini kit (Qiagen) or the RNA Clean and Concentrator 5 kit (Zymo ...
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bioRxiv - Microbiology 2022Quote: ... including either whole fecal pellets or 200-300 uL of contents diluted 1:10 (w/v) in sterile PBS using the MagAttract PowerMicrobiome Kit (Qiagen), and prepped DNA libraries as previously described (36) ...
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bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Total cellular RNA was extracted from 8-day-old wildtype or mutant seedlings grown on 1/2MS medium with RNeasy Mini Kit and QIAshredder (Qiagen). Three independently prepared samples with RNA integrity number greater than 8.0 from each line were sequenced and analyzed at The Centre for Applied Genomics at SickKids Hospital (Toronto ...
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bioRxiv - Microbiology 2022Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufactures protocol ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries (1 ng) were tagmented with the prepared single-loaded Tn5 transposase and afterwards cleaned using MinElute PCR kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 RNA in plasma or in the primary viral isolates was extracted using the QIAamp Viral RNA Mini Kit (Qiagen). cDNA was synthesized using the SuperScript III reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... villosum) (Table 1) were used in RT-PCR tests conducted in Slovenia using the OneStepTM RT-PCR kit (Qiagen, USA) as previously described (Rivarez et al ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteinase K 20mg/ml was added and incubated at 65 for 1 hour and DNA was purified with PCR purification Kit from Qiagen. Then ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were resolved on 0.5x Tris-acetate-EDTA (TAE) 1 % agarose gels and the corresponding bands were cut out and purified using the QIAquick Gel Extraction kit (Qiagen). Restriction enzymes and T4 DNA ligase were obtained from NEB and Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... TetON-plko constructs were digested using AgeI and EcoRI restriction enzyme and the 8.8 kb fragment was purified from a 1% agarose gel using the Qiagen Gel Extraction kit (Qiagen, 28704). Annealed shRNA oligos and digested TetON-pLKO constructs were mixed together with T4 DNA Ligase (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The digested product was loaded on a 1% agarose gel and purified from the gel with PCR and gel purification kit (# 28506 Qiagen). The insert was ligated into the vector with a ratio of 3:1 overnight at 16°C using T4 Ligase (# 300361 Bioconcept) ...
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bioRxiv - Cell Biology 2023Quote: ... Amplicons were resolved on 1% agarose gel by gel electrophoresis and purified using the QiaQuick Gel Extraction Kit (QIAGEN #28706). Purified PCR product was cloned into the TOPO TA Vector and transformed into OneShot TopTen Chemically Competent E ...
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bioRxiv - Molecular Biology 2023Quote: ... TbFUT1 cKO and TbFUT1 cKO F1-γL262P/WT cells grown under permissive and non-permissive conditions and RNA was extracted from 1 ×107 cells using RNeasy RNA extraction kit (Qiagen). RT-qPCRs were performed using Luna® Universal qPCR Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from enrichment material in technical duplicates (1 ml digestate or 0.25 g soil) using the PowerLyzer™ Soil DNA extraction kit (QIAGEN) with modified bead beating using a MP Biomedicals™ FastPrep®-24 (Thermo Fischer Scientific Inc ...
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bioRxiv - Microbiology 2023Quote: ... viral nucleic acids were extracted using the QIAamp Viral RNA Mini Kit (QIAGEN GmbH, QIAGEN Strasse 1, 40724 Hilden, Germany), which purifies RNA and DNA ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... reverse transcription of RNA extracted from OECM-1 cells transfected with miRNA mimics and SC control was carried out using the Quantitect Reverse Transcription Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from DLD-1 cells ectopically expressing EPHB1 mutants using the RNeasy Plus Mini Kit (Qiagen, Germany). First strand cDNA synthesis was conducted with the RevertAid H-minus First Strand cDNA synthesis kit (Thermo Fisher Scientific ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from frozen tissue samples using ceramic beads (Precellys homogenizer, 1× 20 s) in RLT buffer (RNeasy Mini kit, Qiagen) supplemented with 1%ß-mercaptoethanol ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Physiology 2023Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Illumina sequencing-compatible Unique Dual Index adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library quality control was performed using Qubit and TapeStation and sequenced on Illumina MiSeq platform to generate 2 × 250 bp reads.