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Citations for Qiagen :
51 - 100 of 1880 citations for Oxychlordane Unlabeled 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated with 100 μg/ml RNase A solution (Qiagen, 19101). 50 mg/liter Propidium Iodide solution (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... The following day samples were treated with 100 μg/mL RNAse A (Qiagen) for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% Triton X-100) with 50 µl protinase K (>600 mAU/ml, Qiagen) and incubated at 37°C for 30min ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were homogenized in a mixture of chloroform and methanol (2:1) using TissueLyser II (Qiagen) and dried in a Vacufuge (Eppendorf ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmids were transformed into JM109 chemically competent cells and grown overnight in 250 mL of LB broth with 100 µg/mL of ampicillin and purified using the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Synthetic Biology 2019Quote: ... grown over night in 5 mL LB and 100 μg mL-1 ampicillin and DNA were purified using a QIAprep Spin Miniprep Kit (QIAGEN). Sequencing was performed by GATC using the primers in SI Appendix Table S4 ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was extracted from a saturated culture of 100 ml under anaerobic condition (YPD) using the genomic tip-100 kit (Qiagen, Courtaboeuf, FRANCE). Paired-end Illumina sequencing libraries were prepared from sonicated genomic DNA according to manufacturer protocols (Genomic DNA Sample Preparation ...
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bioRxiv - Microbiology 2020Quote: ... 1 ug of RNA was reverse transcribed to cDNA with the QuantiTect reverse transcription kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 μL of lysis buffer (Triton X-100 0.4%, Qiagen Protease 0.1 AU/mL) were added to each well and samples were incubated at 56 °C for 10 minutes and 72 °C for 20 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.5% (v/v) Triton X-100) and 1 mg/ml Proteinase K (QIAGEN, Germany). The solution was incubated at 55°C for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... Following a 2-hour RNase A treatment (Qiagen, 100 mg/ml, 1:1,000 dilution) at 37°C ...
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Microbiology 2020Quote: ... and then with (2) 15 µl Proteinase K [>600 mA/ml] and 5 µl Rnase A [100 mg/ml] (Qiagen, Germany) for 5min at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Spheroblasts were collected at RT and 2,500 g for 5 min and then re-suspended in 1.6 mL per 100 mL culture homemade buffer P1 (according to the manufacturers protocol, QIAGEN, Hilden, Germany). 1.6 mL per 100 mL culture of homemade buffer P2 (according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was prepared via a modified partial phenol-methanol extraction protocol (RNeasy Micro Kit, Qiagen, USA). Purity and quality of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was resuspended in 30 μL of mQ water and incubated with 1 μL of RNase (10 mg/mL prepared from a stock of 100 mg/mL of RNase A from Qiagen, Darmstadt, Germany) at 37 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were purified from 100 ml of overnight culture using a plasmid maxiprep kit (Qiagen). Before transfection ...
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bioRxiv - Plant Biology 2022Quote: ... Afterwards the Genomic Tip 100 was washed twice with 7.5 mL QC Buffer (Qiagen, Germany) per wash ...
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bioRxiv - Genomics 2022Quote: ... to which 4 μL of RNase A (100 mg/mL) was added (Qiagen, Germantown MD). The cell lysate was applied to a DNeasy Mini spin column ...
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bioRxiv - Microbiology 2022Quote: ... Afterwards the pellet was resuspended in 1 ml of 100 % RNAlater/ RNAprotect Tissue Reagent (Qiagen) and kept on ice ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue–methanol mixture was subjected to bead-beating for 45 sec using a Tissuelyser cell disrupter (Qiagen). Extracts were centrifuged for 5 min at 5,000 rpm to pellet insoluble material and supernatants were transferred to clean tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μg/ml proteinase K) for DNA extraction using a QIAamp® DNA Mini Kit (Qiagen). DNA samples were used for bacterial profiling with high-throughput 16S rRNA amplicon sequencing and quantitative polymerase chain reactions.
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bioRxiv - Pathology 2022Quote: ... and 100 μg/ml cycloheximide) and resuspended in 350 μl of buffer RLT plus (Qiagen, 1053393) with β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 ml of PE buffer and 100 μl of EB buffer (MinElute PCR Purification Kit, QIAGEN).
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bioRxiv - Synthetic Biology 2022Quote: ... The pellet was resuspended in G2 Buffer with 20 µL RNase A (100 mg/ml) (Qiagen) and 125 µL RNase Cocktail (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Triton X-100 and 200 µg/ml proteinase K) or DNA Blood Mini kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Systems Biology 2019Quote: ... ∼20 mg frozen tissue was pulverised in chloroform-methanol (400 µl; 2:1 v/v) using a TissueLyser (Qiagen), then the mixture was sonicated for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-dried cells were extracted with 300 μL methanol using a mixer mill (MM 440, Qiagen, Retsch Haan, Germany) at a frequency of 30 s with three repetitions ...
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bioRxiv - Physiology 2021Quote: ... Feces samples were then homogenized in 200 μL 80% (v/v) methanol using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Physiology 2021Quote: ... The hemolymph was diluted in 500 μL 90% (v/v) methanol, homogenized using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Biochemistry 2023Quote: ... 5-6 mg of frozen tissue was homogenized in 250 μL ice-cold acidified methanol (0.1% HCl) and 250 μL water washed with hexanes in a bullet blender (TissueLyser II, Qiagen) with 5 mm stainless steel beads (Qiagen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 µL of samples was mixed with Triton X-100 solution and then incubated with 25 µL of Proteinase K (ProK; Qiagen, 19131; 100 µg/mL in PBS) at 37°C for 30 min prior to Tluc measurement.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed twice with PBS and then resuspended in Propidium Iodide (PI) staining solution (1x PBS supplemented with 50 µg/mL PI, Invitrogen; 100 µg/mL RNase A, Qiagen; and 2 mM MgCl2) and incubated for 30 min at room temperature in the dark) ...
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bioRxiv - Cancer Biology 2023Quote: ... Complementary DNA (cDNA) was synthesized from 1 ug of RNA using the QuantiTect reverse transcription kit (Qiagen, catalog #205311) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The plates were coated with 100 μL of 4 μg/mL murine anti-His mAb (Qiagen, #34660), and after blocking ...
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bioRxiv - Genomics 2022Quote: ... Samples were incubated overnight at 55°C before adding 2 µl of RNaseA (100 mg/ml, Qiagen) and incubated for 15 min at 45°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cotyledons from 100 plants were dissected and ground in 2 ml tubes using a Tissue Lyser (Qiagen) for 1 min 30 sec at 30 Hz before RNA extraction using the RNeasy micro kit (Qiagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 ug were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... after 1 in 4 dilution to each sample prior the extraction and then homogenised samples in 200 μL ice-cold 60% methanol (MeOH) containing 0.01% (w/v) butylated hydroxytoluene (BHT) by using TissueLyserII (Qiagen, Hilden, Germany) at 25 Hertz for one minute and incubating for 20 minutes in an ultrasonic bath (Soniclean Pty Ltd. ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from a 10 ml late-exponential phase culture of DDN032 in HS medium with 125 mM methanol and 100 µg/ml tetracycline as well as the escape mutant in HS medium with 125 mM methanol using a Qiagen blood and tissue kit per the manufacturer’s instructions (Qiagen, Hilden Germany). Library preparation and Illumina sequencing (150 bp paired end reads ...
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bioRxiv - Microbiology 2023Quote: ... cells were collected by centrifugation and quickly washed with ice-cold water to remove the methanol and resuspended in RNAlater solution (Qiagen, 76104) for at least 2 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... Groups of five flies were transferred to 2 mL tubes and were homogenized in 100μl PBS + 0.1% Triton X-100 using a TissueLyser LT (Qiagen). The samples were then spun down at 12,000 rpm and the supernatant transferred to fresh vials and the absorbance of each sample at 603 nm was measured using a NanoDrop ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 100 μg/mL of carbenicillin for 16 hr at 30°C followed by plasmid purification (Qiagen, 12991).
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bioRxiv - Microbiology 2023Quote: ... Pellets were lysed using 100 µg/mL lysostaphin and RNA was extracted with the RNeasy Plus Mini Kit (QIAGEN). Extracted RNA was treated with the Turbo DNA-free Kit (Ambion ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed with 1× PBS and treated with 100 μg/mL of RNase A (Qiagen, USA) followed by staining with 50 μg/mL of propidium iodide (Sigma-Aldrich ...