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Citations for Qiagen :
501 - 550 of 1999 citations for OX7 Anti Thy 1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...
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bioRxiv - Biophysics 2022Quote: ... The YB-1 (1-180) protein fragment was purified following the manufacturer’s recommendations (Qiagen). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Genomics 2023Quote: ... slides were treated with 1 mg/ml RNase A (1 mg/ml, Qiagen, 19101) in 2x SSC for at least 45 min at 37°C and then dehydrated in a 70% ...
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bioRxiv - Molecular Biology 2021Quote: ... 1× QuantiNova RT Mix (Qiagen), 1× QuantiNova SYBR Green RT-PCR Master mix ...
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bioRxiv - Microbiology 2021Quote: ... Strep 1:1000 (Qiagen, 34850).
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 μM oligo-dT18 (Qiagen) and 10 μM random hexamers (Promega).
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bioRxiv - Epidemiology 2019Quote: ... version 1 (Qiagen, Hilden, Germany) with the automated instrument QIAsimphony (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl of DNase (Qiagen) was added ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of QIAzol (Qiagen) was added to each pellet suspension before being transferred to Lysing Matrix B tubes (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 U Hotstar Taq (Qiagen), 1X Hotstar Taq buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 1% SDS (Qiagen P2 buffer). Neutralize lysis buffer by adding 300 μL of 4.2 M Guanidine HCl ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μM TSO (Qiagen). The master mix was dispensed using the MANTIS liquid dispenser followed by mixing for 1 min at 1800 rpm on a plate shaker (Biosan) ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: Total cellular RNA was extracted from mouse placental tissue or HTR cells with an RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: LINE1 and SINEB1 probes were similarly generated except using mouse genomic DNA template extracted from E14 mESCs with DNeasy Blood & Tissue Kits (Qiagen 69504) for PCR ...
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bioRxiv - Cell Biology 2021Quote: Whole DNA was extracted from 15-25 mg of mouse tissue using the DNeasy Blood and Tissue Kit (QIAGEN, cat# 69504) according to the kit’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from mouse retinas using the RNeasy Mini and Micro Kits (Cat No: 74104, 74004, Qiagen, Hilden, Germany). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cell lines or kidney and liver of mouse using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). Approximately 250 ng of DNA was digested with BAMH1-HF (R3136 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected CD31+ BECs from the mouse brain using an RNA extraction kit (#74104, Qiagen, MD, USA). The extracted RNA was stored in RNAse-free water at -80°C until transfer to the Boston University School of Medicine Microarray Core Facility ...
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bioRxiv - Immunology 2020Quote: ... was extracted from two and ten week mouse heart samples stored in RNA Later with the RNeasy fibrous tissue kit (74704, Qiagen, UK). RNA was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). For Gabra2 ...
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bioRxiv - Immunology 2022Quote: ... the livers were perfused 45 hr post mosquito-bite challenge with 1× PBS and total RNA was extracted from the lower right caudate process of the caudate lobe of the liver from each mouse using miRNeasy kit (Qiagen: 217004). The complementary DNA (cDNA ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... hACE2 expression or SARS-CoV-2 nucleocapsid (N) gene in individual mouse organs was determined using QuantiNova SYBR Green PCR kit (Qiagen #208052) in combination of 500 nM of hACE2 gene specific primer set (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from mouse cortices (~0.25 mg per sample) and infected neuronal cultures (5w2d post transduction) using RNeasy kits (Qiagen, 73304&73404) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from patient tissue samples and flash frozen mouse xenograft tumor samples (n = 4) using the RNeasy kit (Qiagen; #74104) and converted to cDNA using the qScript cDNA Synthesis kit (Quantabio ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA from cultured mouse neuro 2a cells was extracted using AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Total RNA from cultured cells and mouse Achilles tendons were isolated with TRIzol reagent and RNeasy Mini Spin Column (Qiagen Sciences) and reversely transcribed into cDNAs using a SuperScript IV VILO Master Mix (Life Technologies).22,30 The relative abundances of genes of interest were determined by TaqMan PCR using primers and probes purchased from Applied Biosystems TaqMan Gene Expression Assays ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was performed using Real-time PCR for RT2 Profile PCR Array Mouse Cytokine and Chemokines using RT2 SYBR® Green Mastermixes (Qiagen). Using the RT2 qPCR Array Data Analysis spreadsheet ...
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bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: 50 mg of mouse spleens were homogenized in 500 μl of the same lysis buffer using the TissueLyser LT (Qiagen, #85600) (50 sec for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Bioengineering 2020Quote: ... Anti-Penta-His Alexa Fluor 488 conjugate was from Qiagen.
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bioRxiv - Cell Biology 2021Quote: ... were functionalized with biotinylated anti-5His antibodies (Qiagen, Valencia CA) and stored with continuous rotation at 4°C in BRB80 (80 mM PIPES ...
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: anti-STrEP-Tag (#34850, Qiagen), monoclonal anti-β-actin antibody (A5441 ...
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bioRxiv - Immunology 2023Quote: ... followed by magnetic depletion using goat anti-rat beads (QIAGEN). For adoptive transfer experiments ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The aqueous phase was collected and mixed at a 1:1 ratio with 70% ethanol (Qiagen). RNA was isolated from this mixture using the RNeasy MinElute Kit (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA of FocnCong:1-1 was isolated using CTAB and 100/G genomic tips (QIAGEN) as described in the 1000 Fungal genomes project (http://1000.fungalgenomes.org) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...