Labshake search
Citations for Qiagen :
251 - 300 of 10000+ citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Cells were seeded in 6-well plates at a density of 1×106 cells/mL and transfected with 200 ng of plasmid using the Effectene transfection kit (Qiagen). Cells were harvested following 3 days of incubation.
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bioRxiv - Cell Biology 2021Quote: ... A total of 5 μl of purified RNA tagged with adapters containing a Unique Molecular Index (UMIs) was converted into cDNA NGS libraries using the QIAseqmiRNA Library Kit (QIAGEN). cDNA was amplified in 22 cycles of PCR and purified ...
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bioRxiv - Cell Biology 2021Quote: ... Then approximately 50 ng of gDNA was harvested from each colony using the QIAamp DNA Micro Kit (Qiagen, no. 56304). The gDNA was PCR amplified in a 50 µl reaction targeting the STADT barcode ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Reverse ttranscription (RT) for miRs was completed with 300 ng of total RNA using a miScript II RT Kit (Qiagen) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The DNA libraries for Illumina NGS were prepared from pooled amplicons by using a QIAseq FX DNA Library Kit (QIAGEN) and were then analyzed by using the iSeq 100 System (Illumina) ...
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bioRxiv - Pathology 2022Quote: ... and 500 ng to 1 μg of DNA was treated with bisulfite using an EpiTect DNA bisulfite kit (QIAGEN, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2021Quote: ... The band corresponding to 257 base pairs was cut out and DNA (NGS library) was extracted using a QIAquick Gel Extraction Kit (Qiagen) according to standard manufacturer protocols ...
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bioRxiv - Genetics 2020Quote: cDNA was synthesized from 250 ng of total RNA per 20 μl reactions using the RT2 First-Strand cDNA kit and random hexamers (Qiagen). PCR for the full-length dACE2 was performed using primers and conditions listed in Table S4 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... we treated 500 ng of RNA with DNase (Perfecta; QuantaBio) then performed cDNA reverse transcription using the QuantiTect Reverse Transcription Kit (Qiagen). We then ran real-time qPCR reactions with SYBR Green detection chemistry using the following reaction mix ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from a pool of 20 mechanically homogenized embryos at 96 hpf for each sample (n=4) using TRItidy G (AppliChem) and 500 ng of cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... The cDNA libraries (1 ng) were tagmented with the prepared single-loaded Tn5 transposase and afterwards cleaned using MinElute PCR kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed on 10 ng cDNA aliquots in technical triplicates per biological replicate (three minimum) using QuantitTect SYBR Green PCR kit (Qiagen). Amplification was normalized to SDHA (succinate dehydrogenase complex flavoprotein subunit A) ...
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bioRxiv - Microbiology 2023Quote: ... The DNA libraries for Illumina NGS were prepared from pooled amplicons by using a QIAseq FX DNA Library Kit (QIAGEN) and were then analysed by using iSeq 100 System (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... 50% confluent HEK 293T cells were transfected with 6000 ng GFP-tagged RhCMV gH-CD4 TM plasmid using Effectene Transfection Reagent Kit (Qiagen) next day ...
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bioRxiv - Molecular Biology 2023Quote: Quantitative RT-PCR was performed using 30 ng of total RNA and QuantiTecT sYBR Green RT-PCR kit (Qiagen, 204243) according to the supplier’s protocol ...
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bioRxiv - Physiology 2023Quote: ... were normalized at 10 ng/µL and the reverse transcription of RNA was done following the manufacturer instructions (miRCURY LNA RT Kit, Qiagen). We added 0.5µl of controls UniSp6 and cel-mir-39-3p to the samples as internal reference to check the efficiency of the reverse transcription and PCR amplification ...
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bioRxiv - Microbiology 2023Quote: A total of 100 ng total RNA was used for library preparation via the QIAseq miRNA Library Kit (#331502, Qiagen) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: Libraries were prepared using Kapa stranded mRNA Hyper Prep sample preparation kits from 500 ng of total RNA isolated using RNeasy columns (Qiagen). The finished dsDNA libraries were quantified by Qubit fluorometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription of 100 ng total RNA from each sample to cDNA was accomplished with the QuantiTect Reverse Transcription Kit (Qiagen) at 42°C ...
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bioRxiv - Genomics 2024Quote: ... Poly(A)RNA (500 ng) from each independent transfection was reverse transcribed to cDNA using QuantiTech Reverse Transcription kit (Qiagen) using a reporter-specific first strand primer (HJ7211 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 75 ng of rRNA was reverse transcribed and amplified with 8 PCR cycles using the OneStep RT-PCR kit (Qiagen) and primers MS2_quant_F and MS2_quant_R (Supplemental Table 5) ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: 400-1000 ng isolated tRNAs were digested in 3 μl aliquot with 20 ng RNase A (QIAGEN) in 10 mM NH4OAc pH 7 or 20 unit RNase T1 in 10 mM NH4OAc pH 5.3 at 37 °C for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: ... as well as pPAx (550 ng) and pMD2 (330 ng) constructs using PolyFect™ transfer reagent (Qiagen). For transduction ...
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bioRxiv - Neuroscience 2021Quote: ... Gene network analyses of RNA-seq data were performed using Ingenuity Pathway Analysis (IPA, Qiagen).
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bioRxiv - Genomics 2021Quote: For the annotations we generated a PacBio Iso-Seq RNA transcriptome: RNA was extracted (Qiagen) and pooled from a mix of males and females from all stages including embryos ...
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bioRxiv - Genetics 2020Quote: ... ChIPs were incubated with RNAse followed by Proteinase K and DNA purified using QIAquick (Qiagen).
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bioRxiv - Genetics 2024Quote: ... ChIP-DNA was treated with 250 μL 1X TE containing 100 μg RNase A (Qiagen) and 5.0 μL of 20 mg/mL Proteinase K (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and 500 ng of purified DNA was subjected to bisulfite treatment using EpiTect Plus DNA Bisulfite Kit (QIAGEN, Cat. No. 59124) according to the manufacturer’s handbook ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA yield was measured by spectrometry (NanoDrop ND-1000; peQLab) and 200 ng/μl were used for reverse transcription with QuantiTect®Reverse-Transcription Kit (Qiagen). We used primer pairs for immune system-related and metabolism-related genes (16 ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized from 500 ng of total RNA using the qScript™ cDNA Synthesis Kit (Quanta/Qiagen, Cat No. 95047). Gene expression analysis was performed using PerfeCTa SYBR Green FastMix (Quanta ...
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bioRxiv - Neuroscience 2019Quote: ... 0.2 ng of the pCMV-TPBG-sport6 vector was transfected using the Effectene transfection reagent kit (Qiagen; Venlo, Netherlands; Cat# 301525), and expression was assessed by immunostaining approximately 24 hours after transfection.
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bioRxiv - Genetics 2021Quote: ... incubated overnight at 37°C in L-broth containing 50 ng/μL ampicillin and DNA isolated using the QIAprep Spin Miniprep kit (Qiagen 27104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... EGF (final concentration 20 ng/ml) was added for indicated times and RNA was extracted using an RNeasy plus kit (Qiagen, 74134) with DNase treatment according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR analysis was performed using 15 ng of cDNA to a final volume of 15 μL reaction with Rotor-Gene SYBR® Green PCR Kit (Qiagen), in a Rotor Gene-Q (R ...
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bioRxiv - Physiology 2021Quote: Approximately 100 ng/µL of total DNA was extracted from 20 mg tissue (QiAMP DNA mini kit Qiagen, Valencia, CA, USA). DNA from bone marrow was extracted from the bone marrow suspension and centrifuged for 10 min at 2000 g ...
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA were reverse transcribed using the QIAGEN OneStep RT-PCR Kit according to the manufacturer’s information (Qiagen, Hilden, Germany). The sequences of primers were as follows ...
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bioRxiv - Pathology 2020Quote: ... Five hundred ng of RNA was reverse transcribed to cDNA using a reverse transcription RT2 First Strand Kit (Qiagen, Hilden, Germany) that is recommended to use in combination with RT2 Profiler PCR Arrays and we followed manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 5 ng of each PCR product was pooled into one tube for purification using the QIAquick PCR purification kit (Qiagen #28106) and eluted into 30 µL Nuclease Free Water ...
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bioRxiv - Cancer Biology 2021Quote: ... Genotyping cell lines and mouse tissue was performed as above with 20 ng of genomic DNA isolated with a Qiagen DNeasy Blood and Tissue DNA Extraction Kit (Qiagen, #69504). All genotyping PCR reactions were performed using 0.4 U (0.08 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... Bisulfite conversion was performed on 100 ng of genomic DNA using the EpiTect Fast DNA Bisulfite kit from Qiagen (Cat.# 59824) and DNA libraries were constructed using the NUGEN Ovation RRBS Methyl-seq System (Cat.# 0353 ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA thus obtained was diluted to 2 ng/µl and specific target miRNAs were amplified by qPCR using the miRCURY LNA SYBR Green PCR kit (Qiagen, 339346). Expression of all targets was normalised against the expression of two reference genes (SNORD48 and U6 ...
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bioRxiv - Cell Biology 2023Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... 250 ng Random Hexamers (Qiagen, Courtaboeuf, France), 4 μl 5× First Strand Buffer ...
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bioRxiv - Genomics 2021Quote: ... identified in RNA-Seq was further validated by a RT2-Profiler PCR array (Qiagen, Hilden, Germany) as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted total RNA-Seq using the Qiagen RNeasy Universal Plus Mini protocol (Qiagen, Hilden, Germany) with DNase I treatment according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... EDGE tests for analysis of RNA-Seq datasets were performed with CLC Genomics Workbench (Qiagen Informatics) as described earlier (Günther et al. ...