Labshake search
Citations for Qiagen :
1 - 50 of 326 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Colonies were selected on LB+Amp agar plates and plasmid was isolated for downstream use (Qiagen Midiprep Kit). pUC57-HaloTag-ACTB-18xenv8-FL and PB510B-1 (System Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial biomass from strains grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Bacterial biomass from isolates grown on SKV agar plates was resuspended directly in 300 μl of PowerBead Solution (Qiagen) and vortexed to mix ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Colonies were washed from each agar plate with M9 and DNA was extracted using a DNeasy kit (Qiagen, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting lawns were scraped off the agar plates and the plasmid libraries were extracted using a QIAprep Spin Miniprep Kit (QIAGEN). The plasmid libraries were subsequently used for yeast transformation.
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bioRxiv - Bioengineering 2024Quote: ... kanamycin-positive colonies were selected from the agar plate and plasmids were extracted using the MiniPrep isolation kit (Qiagen, USA). The cloned construct was eventually confirmed using Sanger sequencing.
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bioRxiv - Biochemistry 2024Quote: ... Colonies were scooped from LB agar plates and ARES plasmid library was isolated following the QIAprep Spin Miniprep Kit (Qiagen) protocol.
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 14-day-old thalli grown on the agar plates using RNeasy Plant mini Kits (QIAGEN, Netherlands). Library preparation and sequencing were conducted by Novogene ...
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bioRxiv - Evolutionary Biology 2024Quote: ... individual colonies on the LB agar plate were readied for miniprep by following the standard protocol described by QIAprep Spin Miniprep Kit (Cat# 27104, Qiagen, Hilden, Germany) Plasmid DNA samples were sequenced following standard sequencing sample preparation and submission described by Eton Bioscience (San Diego ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pylori selective agar (in-house recipe) with the Genomic Tip 100/G (Qiagen, Hilden, Germany). Nextera XT libraries were generated and sequenced in three different runs on MiSeq 2×300bp paired (Illumina ...
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bioRxiv - Microbiology 2020Quote: Bacterial DNA was isolated from overnight cultures on Mueller-Hinton agar using the Genomic-tip kit (Qiagen) and paired-end sequenced with Illumina NextSeq ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted from 10-day-old whole seedlings grown on 1/2X MS media containing 1% sucrose and 0.8% agar (Plant RNeasy kit (Qiagen)) ...
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bioRxiv - Immunology 2020Quote: Ni-NTA plates (Qiagen) were loaded with 2 μg/mL of SARS-CoV-2 S protein in TBS for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... DNA extractions were carried out on ground root and shoot tissue and agar pellets using 96-well-format MoBio PowerSoil Kit (MOBIO Laboratories; Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... iniae QMA0248 grown for 18 h on Todd-Hewitt agar from a master seed stock (non-subcultured) with the Genomic Tip 20 kit (Qiagen). Pre-incubation for 2 h at 37°C of cells suspended in 500 μL 50 mM EDTA containing 200 units of mutanolysin and 2 mg lysozyme was found to improve cell lysis prior to following the manufacturers protocol for purification of high molecular weight DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted from 3 independent infections used to prepare replicates for soft agar and xenograft injection assay using the RNeasy Plus Mini Kit (Qiagen). High RNA quality in all samples was verified using the Bioanalyzer RNA Nano 600 assay (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial cells were harvested from the surface of the cheese agar by using a sterile razor blade and were then immediately placed into 3 mL of RNAProtect Bacteria Reagent (Qiagen) and frozen at -80C until RNA extraction ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (gDNA) was extracted from 24 h-marine agar cultures using the DNeasy Powersoil® Pro Kit (Qiagen, USA) with modifications to the cell lysis steps ...
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bioRxiv - Microbiology 2023Quote: ... The plates were then loaded onto a TissueLyser II plate shaker (Qiagen, Germantown, MD), firmly secured ...
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bioRxiv - Immunology 2022Quote: Ni-NTA HisSorb plates (Qiagen) were coated with 50ng/well of S1 proteins (all from Sino Biological ...
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bioRxiv - Microbiology 2021Quote: Ni-NTA HisSorb plates (Qiagen) were incubated with soluble gH/gL/gO complexes tagged with 6-His overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... using Powerbead Pro Plates (Qiagen) with 0.5mm and 0.1mm ceramic beads ...
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bioRxiv - Neuroscience 2024Quote: ... A handheld vacuum plate (Qiagen) was used to isolate the bead-amplicon by denaturing the dsDNA strand to obtain a single biotinylated strand ...
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bioRxiv - Microbiology 2022Quote: The isolates were recovered overnight on blood agar and genomic DNA was extracted from pure cultures using QIAamp DNA mini kit (Qiagen, Germany) following the manufacturers’ instruction ...
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bioRxiv - Microbiology 2021Quote: ... Isolates were cultured overnight on sheep blood agar and plasmid DNA was extracted using Qiagen Plasmid Midi kit (Qiagen Inc, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... on 96-well Ni-NTA plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and released in PyroMark Q24 plate (Qiagen) pre-loaded with 0.375 μM of sequencing primer ...
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bioRxiv - Immunology 2021Quote: ... cDNA was quantified using 96-well PCR array analysis on a PAMM-150ZA plate (Cytokines & Chemokines) and PAMM-016ZA plate (Type I Interferon Response) (both Qiagen). Quantitative real time-PCR (QRT-PCR ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube containing the two phases was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Synthetic Biology 2021Quote: ... The tube containing the two phases was then fitted in a 96-well storage plate and secured between the top and bottom plates of TissueLyser II (QIAGEN). The components were then mixed for 10 seconds at 15 Hz followed by 7 seconds at 17 Hz ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Physiology 2022Quote: ... Using the Neuronal ion channel plate (Qiagen, UK), 84 ion channels as well as housekeepers were measured in each sample ...
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bioRxiv - Genetics 2023Quote: ... plates were shaken on a TissueLyser II (Qiagen) at 900 rpm (15 rps ...
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bioRxiv - Synthetic Biology 2022Quote: ... and released into a PyroMark Q24 plate (Qiagen) pre-loaded with 0.3μM of sequencing primer ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Cytokine- or vehicle-stimulated primary CD1 astrocyte cultures in 6-well plates and neuron cultures in 24-well plates were lysed and their total RNA extracted using RNeasy Mini Kits (Qiagen 74104) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were plated in 10 cm plates at 5 million cells/plate and the next day transiently transfected with the indicated plasmids using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The plate was loaded with a QIAgility Robot (Qiagen) and run on 7500 Fast Real-Time PCR System (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Plates contained 5 µl of TCL lysis buffer (Qiagen) supplemented with 1%β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... EasyXtal 15-well Tool crystal plates (Qiagen, Manchester, UK) were set up manually for HAdV-D15 ...
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bioRxiv - Biophysics 2024Quote: ... the plate was covered with AirPore tape sheets (Qiagen), and the plate was shaken at 250 RPM at 37 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... or the DNeasy® 96 Blood & Tissue plate kit (Qiagen) according to supplier’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... and loaded on RT2 PCR profiler plates (Qiagen, PAHS-078Z). Results were analyzed using the GeneGlobe Data Analysis Center (Qiagen).
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bioRxiv - Genomics 2020Quote: ... transfected cells were lysed on plate in RLT buffer (Qiagen) and stored at -80°C ...
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bioRxiv - Genomics 2024Quote: ... and mRNA was captured with a Turbocapture plate (Qiagen 72251). Reverse transcription and linear amplification were conducted directly on immobilised mRNA ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μL of 0.1 M glycine-HCL buffer pH 2.7 ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCL buffer pH 2.7 ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum ...
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bioRxiv - Immunology 2020Quote: ... washed with PBS using a 96-well plate manifold base (Qiagen) connected to the vacuum and eluted into 96-well PCR plates using 86 μl of 0.1 M glycine-HCL buffer pH 2.7 ...