Labshake search
Citations for Qiagen :
401 - 450 of 10000+ citations for Mouse Vasoactive intestinal polypeptide receptor 2 VIPR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2019Quote: ... rRNA depletion of the poly(A)-tailed RNA isolated from yeast cells and mouse tissues was subsequently performed using a GeneRead rRNA Depletion kit (Qiagen), according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2020Quote: ... 4 – 4.5 week-old neurons co-cultured with mouse astrocytes were lysed and total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...
-
bioRxiv - Genomics 2021Quote: ... For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted from sorted and unsorted mouse fecal-derived bacterial cells using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as per manufacturer instructions ...
-
The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: Total RNA from WT and ΔIg3-MuSK mouse soleus and TA was extracted using a RNeasy Fibrous Tissue mini kit (Qiagen) for non-stranded RNA library preparation and sequencing (Genewiz).
-
bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
-
bioRxiv - Microbiology 2022Quote: Bacterial RNA from mouse GI contents (approximately 250 mg) was extracted using RNeasy® PowerMicrobiome® kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: Microglia cultures from mouse pup spinal cords were lysed for total RNA extractions using RNeasy Plus Mini Kit (74134, Qiagen). cDNA generation was performed using High-Capacity cDNA Reverse Transcription Kit (4368814 ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA from the mouse whole hypothalamus and epididymal white adipose tissue were extracted using the RNeasy Universal Plus Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
-
bioRxiv - Cell Biology 2023Quote: ... Genomic DNA of E8.5-E9.5 mouse embryos and HFF-hiPS cells (used as a positive control) were extracted using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA was prepared from mouse and rat embryonic molar tooth RNA (extracted using RNeasy Plus Micro kit, Qiagen, Düsseldorf, Germany). cDNA constructs were inserted in TOPO II PCR-plasmids and using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: Genomic DNA from 24 months old CreER and nmrHAS2 mouse livers was purified using a DNeasy Blood & Tissue Kit (Qiagen). One hundred nanograms of purified genomic DNA were used for the methylation measurement ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA from mouse embryos were isolated using RNeasy plus micro kit with on-column DNase I digestion method (QIAGEN). cDNA libraries were produced by using an Ovation RNAseq amplification kit Ver ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cell-free DNA from cell culture supernatants and mouse plasma was manually isolated using the QIAamp MinElute ccfDNA Mini Kit (Qiagen), while cfDNA from patient plasma samples was isolated with QIAsymphony SP using the QIAsymphony DSP Circulating DNA Kit (Qiagen) ...
-
THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: qRT-PCR was performed on total RNA extracted from adult mouse tissues or organoid cultures using the RNeasy Mini Kit (Qiagen). A DNAse I treatment (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA from mouse cortical tissues and primary cortical neurons were isolated using QIAshredder and RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA from the human cell lines and mouse tumor samples was extracted using RNeasy Plus Mini Kits (Qiagen, 74134) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA from the developing mouse cerebral cortex and isolated GFP-positive cells were prepared using the RNeasy mini kit (Qiagen). cDNA was synthesized using SuperScript III reverse transcriptase (Thermo) ...
-
bioRxiv - Immunology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using QIAseq fast select multi-RNA removal kit for mouse RNA(Qiagen) and then RNAseq libraries were made using NEB Next Ultra II Directional RNA library prep kit for Illumina ...
-
bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
-
bioRxiv - Biophysics 2021Quote: ... and the bindingPCA plasmid containing the ligand GAB2 or CRIPT fused to DHFR1,2 (pGJJ006 and pGJJ066 respectively) were digested with the same enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN).
-
bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2020Quote: ... TRAF3 KO1 and TRAF3/p100 double KO cells from 2 experimental repeats was isolated using the RNeasy Mini Kit (Qiagen). RNA quality control was performed using a 2100 Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... The pellets were resuspended with OMV buffer and re-pelleted again by centrifugation at 200,000 g for 2 h at 4°C and lysed with Qiazol followed by RNA isolation with the miRNeasy kit (Qiagen) to obtain total RNA including the small RNA fraction ...
-
bioRxiv - Developmental Biology 2021Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
-
bioRxiv - Molecular Biology 2021Quote: Genomic DNA was extracted from fresh leaves of 2 to 3-week-old seedlings for all lines using a Qiagen MiniPrep Kit (Qiagen). Genotyping of all lines was performed using a custom lentil exome capture assay as described in Ogutcen et al ...
-
bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
-
bioRxiv - Genomics 2019Quote: Total RNAs from the knockdown (shCTCF#1 and shCTCF#2) and control (shLuc) cells were extracted using miRNeasy Micro Kit (QIAGEN). cDNA was synthesized by using oligo (dT)20 and SuperScript III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Genomics 2019Quote: ... Purified products were loaded onto a 2% E-gel and gel extracted using a QiaQuick Gel Extraction kit (Qiagen 28704). The molarity of the gel-extracted PCR product was quantified using KAPA library quant (KK4824 ...
-
bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
-
bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from 2 mL of overnight growth in BHI broth using the DNeasy Blood and Tissue kit (Qiagen). Genome sequencing of the two isolates was performed on a Miseq platform using Illumina technology ...
-
bioRxiv - Biochemistry 2019Quote: Genomic DNA was extracted from 2 g of frozen Annona muricata seed powder with the DNEasy Mericon Food Kit (QIAGEN) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
-
bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 nucleocapsid (N) gene in homogenized tissues were determined using QuantiNova SYBR Green PCR kit (Qiagen) along with 2019-nCoV RUO Kit (Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... Mitochondria were purified from approximately 2*106 cultured HeLa cells continuously expressing the mitochondrial matrix marker su9-mCherry using the Qproteome Mitochondria Isolation Kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... The elution supernatants were combined (a 20 μL elute sample was taken and combined with 20 μL 2 × SDS loading buffer) and RNAs were extracted using a miRNeasy kit (QIAGEN) according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2020Quote: ... Nested PCR was then performed with 2 μl of cDNA in 25 μl reactions with the HotStar Taq Plus Kit (QIAGEN), using 5’ leader sequence–specific and 3’ IgG-specific primers (64) ...
-
bioRxiv - Bioengineering 2021Quote: ... dsDNA product was run on a 2% agarose gel and extracted with the Qiaex II Gel Extraction Kit (Qiagen, 20021). dsDNA was transcribed to RNA using 5 reactions of the Durascribe transcription kit as described above ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were harvested 2 days post-transfection and genomic DNA (gDNA) was purified with the QIAamp DNA Blood Mini Kit (Qiagen). The genomic region surrounding the pegRNA target site was then amplified by PCR with the following primers ...
-
bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
-
bioRxiv - Neuroscience 2021Quote: ... (n=3 biological replicates) and TACs (hGFAP::GFP-, CD133-, EGFR+, CD24-) (n=2 biological replicates) using the miRNeasy kit (Qiagen). miRNAs were pre-amplified and profiled using TaqMan® Array Rodent MicroRNA A Cards v2.0 A as specified by the manufacturer at the Genome Technology Center of New York University Langone Medical Center ...
-
bioRxiv - Neuroscience 2021Quote: ... PCR products were run on a 2% agarose gel and gel bands were manually extracted and purified using QIAquick PCR & Gel Cleanup Kit (QIAGEN) according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted from SK-N-AS and SK-N-BE(2) xenograft tissue using the RNeasy Mini Kit (Qiagen) and quality control was performed with Agilent Tapestation according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Second PCR products were pooled and gel purified from a 2% agarose gel using the MinElute Gel Extraction kit (QIAGEN). Purified product concentration was measured using a Qubit (Thermo Scientific) ...