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Citations for Qiagen :
51 - 100 of 10000+ citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted from the T cells using the DNeasy Blood and Tissue kit (Qiagen, 69504) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from FACS-sorted T cell populations using genomic DNA isolation kits (Qiagen). Purified genomic DNA was subjected to bisulfite treatment with the Qiagen EpiTect bisulfite kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from the T cells using the RNeasy Mini RNA Isolation Kit (Qiagen). TCR α- and β-chain genes were reverse transcribed and amplified from the RNA using the SMARTer RACE Kit (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated from unedited and edited T cells using the RNeasy Mini Kit (Qiagen) and quality was determined using a BioAnalyser (Agilent ...
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bioRxiv - Immunology 2023Quote: ... DNA was extracted from library-edited T cells using a DNeasy Blood & Tissue Kit (Qiagen, #69504) according to the manufacturer’s protocols with buffer AL ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from T cells using the RNeasy Mini kit (#74104, Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from B7.H3 CAR-T cells using the RNeasy Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from CD3+ T-cells using the Qiagen RNeasy Plus Mini Kit or EN AllPrep Mini Kit with on column DNaseI (Qiagen) digestion ...
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... RNA from CD4+ T cells (2×106 cells per condition) was extracted by using the RNeasy Plus Mini kit (QIAGEN). Extracted total RNA was quantified using the Qubit broad range RNA assay ...
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bioRxiv - Cell Biology 2019Quote: ... by retrotranscription of RNAs from HeLa and HEK293-T cells using the QuantiTect Reverse Transcription kit (Qiagen) followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech) ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was extracted from T cells using a DNeasy Blood & Tissue kit (Qiagen, Cat no. 69506). PCR amplification of the region of gDNA containing the CRISPR editing locus was performed using the SequalPrep Long PCR Kit with dNTPs (Invitrogen ...
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bioRxiv - Immunology 2021Quote: DNase I-treated total RNA was prepared from sorted T cells using RNeasy RNA isolation kit (Qiagen) and cDNA was synthesized using an iScript cDNA synthesis kit (Biorad) ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from in vitro activated CD4+ T cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98) with the DNeasy Blood & Tissue Kit (QIAGEN). Each HIV reaction contained 825 ng of gDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lines and mouse tails using DNeasy Blood & Tissue kit (Qiagen). Exome libraries were prepared by the Advanced Sequencing Facility at the Crick and sequenced using an Illumina HiSeq 4000 using 100 base pair paired-end reads ...
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bioRxiv - Genomics 2021Quote: Total RNA was collected from 2×106 CAR T cells with the RNEasy Plus Mini isolation kit (Qiagen). Library preparation and RNA-seq was performed by BGI America (Cambridge ...
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bioRxiv - Genetics 2023Quote: ... and DNA and RNA were extracted from CD4+ T cells using the AllPrep Universal Kit (Qiagen, Hilden, Germany). Cell-associated total HIV DNA and unspliced RNA were quantified by an in-house quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Immunology 2023Quote: ... These purified CAR-T cells were lysed for bulk RNA extraction using RNeasy Plus mini isolation kit (Qiagen). Library preparations were performed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: RNA isolation of platelet releasate treated MHEC5-T cells was performed using the RNeasy Mini Kit (#74106; Qiagen). The isolation was conducted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from 5 x 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from T-ALL cells collected purification using RNeasy Plus Mini or Micro Kit (Qiagen). cDNA were generated using SuperScript® VILOTM cDNA Synthesis Kit (ThermoFisher Scientific).Real-time PCR reactions were carried out using Power SYBR® Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reactions were performed using the QuantiFast SYBR Green PCR Kit (QIAGEN) and the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Transgenic animals were identified by polymerase chain reaction of tail DNA (DNeasy kit, Qiagen, Hilden, Germany) using primers for Cre (Schönig et al. ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from the cultured cells or ex vivo CD4+ T cell samples using DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
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bioRxiv - Cancer Biology 2019Quote: ... Pathways significantly affected in non-T cell-inflamed or non-T cell-inflamed-enriched mutated genes were identified by IPA® (QIAGEN Inc., Germany).
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bioRxiv - Immunology 2019Quote: ... mock-infected or HIV-infected CD4+ T cells was isolated using the RNeasy Mini Kit with QIAshredder columns (Qiagen). cDNA was produced using the SuperScript III First Strand Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... TCRαβ+CD4-CD45RClow/- T cells from Il34+/+ and Il34-/- rats were extracted using RNeasy Mini Kit (QIAGEN, Hilden, Germany) and protocol of 3’ DGE RNA sequencing was performed as previously described (16) ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from 5 million transfected Jurkat T-cells using the DNeasy Blood and Tissue kit (Qiagen) and quantified by Quant-iT™ PicoGreen™ dsDNA Assay kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from multiples of 4 million resting CD4+ T cells according to the manufacturer’s instructions (QIAamp DNA Mini and Blood Mini Kit, QIAGEN). Amplification of NFL genomes was performed by limiting dilution ...
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bioRxiv - Immunology 2020Quote: ... human sputum cells or mouse lung tissue using an RNeasy kit (Qiagen). 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from mouse cells using the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Cancer Biology 2019Quote: ... T cells were sorted directly into 250μl RNAprotect buffer (Qiagen), spun down for 1 minute at 2000 RPM ...
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bioRxiv - Immunology 2021Quote: ... we extracted total RNA from CD4+/CD8+/CD3+ T cells (from spleen or bone marrow) of Foxp3-GFP or C57BL/6 mice using RNeasy Micro Kit (Qiagen) and cleaned from excess DNA with DNAse 1 enzyme (Promega) ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from flow cytometry-sorted T cells using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from freshly isolated T-cells on day 7 of treatment from spleens using RNeasy Kit (Qiagen). For each group ...
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bioRxiv - Immunology 2020Quote: ... was isolated from previously sorted T cells and monocytes (CD19-CD3+/CD14+)30 using the QiaAmp DNA mini kit (Qiagen), and the concentration was measured on Nanodrop.
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Immunology 2023Quote: ... Immune cells were FACS-sorted for APC-enriched samples or T cell-enriched samples and RNA was subsequently extracted (RNeasy Mini Kit, Qiagen). RNA was analyzed on an nCounter MAX Analysis System (Nanostring ...
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bioRxiv - Immunology 2024Quote: ... T-cell pellets were resuspended in 350 µL of RLT+ buffer from the Qiagen RNeasy plus mini kit (Qiagen, 74136) and RNA was extracted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Cancer Biology 2019Quote: The cDNA of wild-type ecATCase and regulatory chain of ecATCase-holo were amplified by PCR (Qiagen Kit) using BL21(DE3 ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was isolated from mouse B cells using the DNeasy kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... + 5% beta-mercaptoethanol using a bead mill (Qiagen). Samples were heated at 95° for 5 minutes to denature proteins ...