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Citations for Qiagen :
551 - 600 of 1364 citations for Mouse Anti Hepatitis C Virus E1 Antibody 1879 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... 100ng of RNA was converted to cDNA for 1h at 37 °C using the Omniscript RT kit (Qiagen) and random primers (Promega) ...
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bioRxiv - Biophysics 2022Quote: ... The suspension was spun down at 16,000 r.c.f at 4 °C for 30 min and the supernatant was applied 5 ml Ni-NTA resin (Qiagen)/ L culture medium ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Granuloma homogenate was taken for RNA isolation using RNeasy Plus Kits and stored at −80°C (Qiagen, USA). Quantification of viral burden was done using TaqPath 1-step RT-qPCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... the pellet was stored at -80°C before RNA extraction using the Qiagen RNeasy Mini kit (Qiagen, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... for 30 min at 37°C and cleaned up with the RNeasy MiniElute cleanup kit (Qiagen, Hilden, Germany) following the manufacturers’ instructions ...
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bioRxiv - Immunology 2019Quote: ... and purified in buffer C (20 mM Tris, 100 mM NaCl, pH 7.5) by nickel-affinity chromatography (Qiagen) followed by size-exclusion chromatography on a Superdex 200 column in buffer D (20 mM HEPES ...
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bioRxiv - Pathology 2020Quote: ... diffracting crystals of P1_102-157 were obtained at 4°C from condition 7 of the Classic kit (Qiagen) containing 0.1 M tri-Sodium citrate pH 5,6 ...
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bioRxiv - Microbiology 2021Quote: ... for 30 minutes at 37°C followed by beadbeating with 50μg 0.1mm zirconium beads for 6 minutes on the Tissuelyzer II (Qiagen) prior to loading onto the Qiacube HT ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 0.6 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture (1 ml beads per 1 l expression culture was used in case of Drosophila BiP ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C for 1 hour and cleaned up with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 37 °C) with 50 μg RNA sample and purified using RNeasy Protect Mini Kit (catalog no. 74124, Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL2-Rα ectodomain was produced with C-terminal 6xHis tag and purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2022Quote: ... for 24 hours at -20°C prior to DNA/RNA extraction with AllPrep DNA/RNA Mini Kit (Qiagen). Cryopreserved patient ascites cells and PDX cancer spheroids were thawed and rinsed in serum free RPMI before RNA/DNA extraction ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was eluted from the column using 5 mL of prewarmed (50°C) QF Buffer (Qiagen, Germany). DNA was precipitated by adding 0.7 volumes of isopropanol ...
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bioRxiv - Neuroscience 2022Quote: ... Reaction was incubated at 37 °C for 30 min and purified using the QIAGEN MinElute Kit (28004 QIAGEN) according to the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... Tregs were pelleted and stored at -80°C in RTL lysis buffer (RNeasy micro kit, Qiagen, Hilden, Germany) with β-mercapto-ethanol (1:100) ...
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bioRxiv - Genetics 2022Quote: Liver samples were stored frozen at −80 °C before RNA extraction with the RNeasy kit (QIAgen, Crawley, UK) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... homogenized samples were stored at −20 °C prior to extraction using the DNeasy® PowerSoil® Kit (Qiagen) following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... at -20°C or immediately processed for RNA using the RNeasy Mini Kit as per manufacturer’s protocol (Qiagen). Real-time analyses were performed as previously described (Van Otterloo et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Genomics 2023Quote: ... or the buffy coat of the BALB/c strain using the QIAsymphony DNA Mini Kit (cat:931236, Qiagen). The extracted gDNA was sheared to 150 bp in size using a Covaris LE 220 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Immunology 2024Quote: ... 2 × 104 cells were sorted in triplicate per sample and stored at −80°C in RLT buffer (Qiagen). RNA was isolated using the Qiagen RNeasy Micro Kit ...
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bioRxiv - Biochemistry 2023Quote: ... for 45 min at 4°C and the supernatant was mixed with 3 mL Ni-NTA agarose (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated at 37 °C for 15 min and then quenched by adding RLT buffer (Qiagen). The RNA was then extracted using RNeasy kit (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was stored at −80°C until further clean-up using RNeasy Mini kit (Qiagen, catalog #74104) manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... One hour of tagmentation at 37°C was followed by DNA extraction using MinElute PCR Purification Kit (Qiagen). Extracted DNA was subjected to PCR amplification using unique primers sets (Nextera XT v2 Full set (N7-S5)) ...
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bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: Mouse posterior cortex was disrupted for RNA isolation using the TissueLyser (QIAGEN, Hilden, Germany) for 2 x 2 min at 20 Hz as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: Purified RNA was isolated from mouse skin using the RNeasy Plus Mini Kit (Qiagen) after pulverization of the frozen skin tissue and homogenization with the QIAshredder system (Qiagen) ...
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bioRxiv - Biochemistry 2019Quote: Fecal DNA from mouse colon was extracted by QIAamp PowerFecal DNA Kit (Qiagen, Germany) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from mouse hippocampal samples using a RNeasy Mini kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and from mouse tissue (liver, retina, RPE) using a DNeasy Blood & Tissue kit (Qiagen). Target sequences were PCR-amplified using 2X Taq PCR Smart mix (SolGent) ...
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bioRxiv - Physiology 2020Quote: Whole RNA was extracted from mouse orbital fat samples using RNeasy mini-kits (Qiagen). cDNA was prepared using Superscript III first-strand synthesis kit (ThermoFisher) ...
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bioRxiv - Pathology 2022Quote: Total RNAs were obtained from cells or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed with specific primers for mouse IFN-α11 (PPM03050B-200, Qiagen), mouse β-actin (PPM02945B-200 ...
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bioRxiv - Systems Biology 2022Quote: ... DNA was isolated from mouse tissues using the DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Total RNA from mouse lungs was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: Total RNA (100–200 islets/mouse) was extracted using the RNeasy Mini Kit (Qiagen). RNA quality and quantity was measured on the Agilent 2200 TapeStation System ...
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bioRxiv - Molecular Biology 2020Quote: Total mouse heart RNA was isolated with an RNeasy mini kit (Qiagen, Valencia, CA) or RNAqueous® Micro RNA isolation kit (Ambion ...
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bioRxiv - Immunology 2019Quote: ... RT2 Profiler™ PCR Array Mouse Autophagy was purchased from Qiagen (Catalog # PAMM-084Z). RNA extracted from the brain of pups born to infected dams were analyzed for the expression of autophagy-related genes following the manufacturer’s instruction and as previously described (13).
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from mouse MLL-AF9 cells by RNeasy Micro Kit (Qiagen), 72 hours after transduction of the cells ...
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bioRxiv - Molecular Biology 2022Quote: ... for mouse samples and cell lines or with miRNeasy Mini Kit (Qiagen, Hilden, Germany) for human tissue samples ...
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bioRxiv - Neuroscience 2023Quote: RNA from brain organoids and mouse tissue was extracted with RNeasy Mini Kit (Qiagen) for mRNA detection ...