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Citations for Qiagen :
201 - 250 of 2117 citations for Mouse Anti Hepatitis C Virus Core Protein Antibody 1862 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Biochemistry 2019Quote: ... proteins were blotted onto PVDF membrane and examined with an RGS-His4-antibody (Qiagen) and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3 ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... located at the UCSF FACS core and subjected to RNA preperation with RNeasy Plus micro kit (Qiagen 74034). The quality of RNAs was evaluated with 2100 Bioanalyzer Instrument (Agilent Technologies) ...
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bioRxiv - Immunology 2020Quote: ... MuSK1A crystals were grown at 18 °C by vapor diffusion and after ≈ 4 days grew from drops consisting of 100 nL protein plus 100 nL of a reservoir solution consisting of 20% w/v PEG 6K (Precipitant) and 0.1 M BICINE 8.5 pH (Buffer) from the JCSG Core I (Qiagen) screen ...
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bioRxiv - Genetics 2019Quote: DNA was extracted from blood or cell lines using the Puregene Blood Core Kit B (Qiagen, Cat#158467). PCYT1A exons were PCR-amplified from SMD-CRD patient genomic DNA with Accuprime Taq polymerase (Invitrogen ...
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bioRxiv - Genetics 2022Quote: Genomic DNA from both sperm and saliva samples was extracted using the Puregene Core Kit A (QIAGEN, #1042601) according to the manufacturer’s instructions with minor modifications previously described in (Arbeithuber et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Screening plates were set up by a sitting-drop vapor diffusion method using JCSG Core I-IV (Qiagen), PEG Suite I-II (Qiagen) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from two batches of 50 adult females using the PureGene Core Kit A (Qiagen) and the two gDNA extracts were then pooled in equal proportion into a single sequencing library ...
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bioRxiv - Plant Biology 2022Quote: ... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2023Quote: ... The antibodies were anti-RGSHis (Qiagen, Cat. No. 34650) and peroxidase-conjugated anti-mouse antibody (Dako ...
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bioRxiv - Biochemistry 2022Quote: ... an anti GAPDH monoclonal antibody (SC-47724) or an anti RGS HIS6 HRP (QIAGEN). When necessary ...
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell debris were removed by centrifugation (35,000 g at 4°C for 20 min) and the N-terminal His-tagged proteins were purified from the supernatant using NiNTA agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... C-terminal His-tagged proteins were purified by gravity-flow chromatography on a nickel nitrilotriacetate (Ni-NTA) agarose resin (Qiagen) according to the manufacturer’s recommendations followed by an exclusion chromatography on a Superdex75 column connected to an ÄKTA Purifier™ (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA from these samples was obtained with the QIAamp Mini Elute Virus Spin Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 2 μl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was extracted from each sample using QIAgen DSP virus/pathogen Midi kits (QIAGEN) on a QIASymphonyXP laboratory automation instrument platform ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from 200 μL of specimen using the EZ1 DSP Virus kit (Qiagen) on the EZ1 Advanced XL instrument (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: Tissue homogenates for virus titration were generated using the Tissue Lyzer II (Qiagen, Gaithersburg, MD). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... RNA was extracted using QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). qRT-PCR was then performed using AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... viral RNA was extracted from virus stocks using the Qiagen Viral RNA Mini Kit (Qiagen) and was used as a template for multi-segment RT PCR as described previously (31) ...
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bioRxiv - Genomics 2022Quote: ... Viral nucleic acids were then isolated using QIAamp MinElute Virus Spin Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions with some modifications described (Cosentino et al. ...
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bioRxiv - Microbiology 2023Quote: ... Samples for analysis of virus attachment were directly collected by incubating in RLT buffer (Qiagen) for 10 min at room temperature ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and nucleic acid was extracted using EZ1 Advanced XL extraction with a Virus Card (QIAGEN). Six of the 20 FACS populations did not generate sufficient DNA and were not subsequently sequenced ...
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bioRxiv - Microbiology 2023Quote: ... DNA extraction was performed on the QIAsymphony using the DSP Virus/Pathogen Mini Kit (Qiagen). Library preparation performed using Nextera XT (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: Viral genomic RNA was isolated from concentrated virus stocks with viral RNA isolation kit (Qiagen). RNA was treated with Turbo DNase and inactivation beads (Ambion) ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen, Toronto, ON, Canada). Then ...
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bioRxiv - Biochemistry 2023Quote: ... and mouse anti-Phospho-Serine Q5 (catalogue number: 37430) was from Qiagen. Horseradish peroxidase-conjugated secondary antibodies against rabbit (catalogue number ...
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bioRxiv - Cell Biology 2019Quote: The decellularized Balb/c mouse pancreas scaffolds were washed in PBS and homogenized-lysed for 1 hr in Tissue homozilyser II (Qiagen). The ECM proteins were dissolved in lysis buffer containing Tris HCl 0.06M ...
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bioRxiv - Microbiology 2019Quote: ... Sequencing was performed by the University of Pittsburgh HSCRF Genomics Research Core and analyzed using CLC Genomics Workbench (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... The complex (12 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Microbiology 2020Quote: ... The complex (7.5 mg/ml) was screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our custom-designed robotic CrystalMation system (Rigaku ...
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bioRxiv - Cell Biology 2021Quote: ... Up-Stream Regulators enriched in differentially expressed genes (no logFC cutoff, FDR < 0.05) in direct and indirect interactions were investigated by performing Core Analysis using (QIAGEN).
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bioRxiv - Molecular Biology 2022Quote: ... The libraries were built with 10.25 μl of DNA as starting material and using the GeneRead DNA Library I Core Kit (Qiagen, half of the volumes suggested by the manufacturer instructions were used ...
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bioRxiv - Immunology 2022Quote: ... Sorted LECs were submitted to OHSU’s Gene Profiling Shared Resource Core for RNA Isolation using RNeasy Micro Kit (Qiagen); RNA quality was assessed using Bioanalyzer 2100 with RNA 6000 Pico chips ...
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bioRxiv - Cancer Biology 2019Quote: ... 2mm diameter and 3mm3 deep cores were obtained and DNA was extracted using the GeneRead DNA FFPE kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA was isolated from a culture grown on R2B using the Puregene Core kit A (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the pathways relevant to the significantly differentially expressed genes (FDR <= 0.05) were identified using the Core Analysis of the IPA software (QIAGEN Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from FFPE tumor cores using RNeasy FFPE kits according to the manufacturer’s protocol (QIAGEN, Germantown, MD). RNA-seq libraries were generated using TruSeq RNA Access Library Prep Kits (TruSeq RNA Exome kits ...
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bioRxiv - Immunology 2023Quote: ... The protein complexes were adjusted to 8.6–12 mg/ml and screened for crystallization using the 384 conditions of the JCSG Core Suite (Qiagen) on our robotic CrystalMation system (Rigaku ...
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...