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Citations for Qiagen :
401 - 450 of 1341 citations for Mouse Anti Crimean Congo Hemorrhagic Fever Virus Gn Protein JE12 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The protein was isolated using a Ni-NTA column (Qiagen). 25 μg of isolated protein was used to immunize mice by subcutaneous injection with complete Freund’s adjuvant and boosted three times with incomplete Freund’s adjuvant to generate PfATP4 antibody.
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by cation exchange chromatography using Source 15S Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were affinity-purified with Ni-NTA Agarose Resin (Qiagen), followed by affinity-purification with amylose resin (New England Biolabs) ...
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bioRxiv - Bioengineering 2020Quote: ... the membrane was incubated with freshly prepared primary antibody solution (1:1,000 dilution for anti-Alix [ab117600, Abcam], anti-Tsg101 [ab30871, Abcam], anti-Calnexin [ab22595, Abcam], anti-His [34660, Qiagen] ...
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bioRxiv - Immunology 2020Quote: ... Mouse and Human IFN I RT2 Profiler PCR Arrays (Qiagen) were performed and relative expression determined using the ∆∆CT method and normalized for 5 housekeeping genes according to manufacturer’s guidance.
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with AllStars Mouse Negative Control siRNA (Qiagen) or siKras (siKRAS_234 as described by Yuan et al.59 ...
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bioRxiv - Developmental Biology 2021Quote: ... Total mouse RNA was isolated using RNeasy mini kit (Qiagen) and reverse transcribed to cDNA using an oligo (dT ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen mouse liver were first homogenized using TissueLyser II (Qiagen) and then lysed in 1X Laemmli buffer ...
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bioRxiv - Immunology 2022Quote: ... or from frozen mouse BMDMs using RNeasy Mini kit (Qiagen). Reverse transcription (RT ...
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bioRxiv - Developmental Biology 2023Quote: ... RT² Profiler™ PCR Array Mouse Neurogenesis (330231, Qiagen, Germany) was performed according to the manufacturer’s instructions on LightCycler 96 (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Physiology 2023Quote: ... RT2 Profiler PCR Array for Mouse Fibrosis (PAMM-120Z, Qiagen) and Qiagen GeneGlobe Analysis was used to evaluate fibrosis between normal saline and experimental groups at each time point.
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bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Initial screens were performed using Nucleix and Protein Complex suites (Qiagen) in a sitting-drop setup ...
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bioRxiv - Microbiology 2021Quote: ... the proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... E2 and polyPRM5 proteins were purified using Ni NTA-agarose (Qiagen) followed by cation exchange (Source15S ...
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bioRxiv - Cancer Biology 2020Quote: ... The bacterial His-tagged proteins were purified under denaturing conditions (Qiagen) and refolded by dialysis as described previously (22) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were immobilized on a glutathione agarose gravity flow column (Qiagen) washed 5 times with PBS solution and eluted in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2020Quote: GRF5 protein sequences were aligned using CLC DNA Workbench 8.0 (Qiagen) with the following settings ...
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bioRxiv - Immunology 2021Quote: ... The proteins were captured using Ni-NTA (Qiagen, Germantown, MD, USA), washed in EQ buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Fusion proteins were purified from clarified lysate by Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen) and the histidine tag was subsequently removed by thrombin digestion (10 U/mg of protein) ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins in the supernatants were purified using Ni-NTA beads (Qiagen) according to (62) ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by FPLC using a Ni-NTA column (Qiagen), PBE-94 column (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA was extracted with DNA/RNA/Protein Mini Kit (Qiagen). The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was purified from culture supernatant with Ni-NTA (Qiagen), biotinylated with in-house purified BirA enzyme ...
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bioRxiv - Microbiology 2019Quote: ... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tagged protein was purified using Ni-NTA resin (Qiagen) following the manufacturer’s instructions and was used for rabbit immunization ...
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bioRxiv - Genomics 2021Quote: ... The proteins were bound to a Ni-NTA sepharose beads (Qiagen), extensively washed with binding buffer and eluted in buffer containing 50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2021Quote: ... and proteins were purified with Ni-NTA resin (Qiagen, Cat# 151010181) and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... The solubilized protein was then purified using Ni-NTA agarose (Qiagen). The purity was confirmed (>98% ...
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bioRxiv - Microbiology 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen), the eluted fractions containing S protein were pooled ...
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bioRxiv - Microbiology 2021Quote: ... cruzi proteins attached to addressable Liquichip Ni-NTA beads (Qiagen, CA) and T ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion protein was affinity purified using Ni-TNA agarose (Qiagen). Details are provided in the Supplementary Information methods section.
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bioRxiv - Microbiology 2022Quote: ... Protein was purified under native conditions according to the QIAexpressionist (Qiagen) protocol ...
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bioRxiv - Molecular Biology 2022Quote: The His-tagged protein was purified by Ni-NTA agarose (Qiagen). Eluted proteins from beads were then applied to the NGC chromatography system (Bio–Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Protein was purified from sonicated lysates using Ni-NTA resin (Qiagen) and gravity flow ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris gels (Novex, Qiagen) using MOPS running buffer (Novex ...
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bioRxiv - Developmental Biology 2023Quote: ... After the protein digestion with Proteinase K (QIAGEN, Cat. No. #158920) and RNA digestion with RNase A (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified by affinity chromatography using Ni-NTA resin (Qiagen) and a gravity column (Biorad) ...
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...