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Citations for Qiagen :
101 - 150 of 10000+ citations for Mouse 60S ribosomal protein L22 like 1 RPL22L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... primers with an annealing temperature of 60 C for 45 seconds and PCR products purified via the QIA Quick PCR Purification Kit (Qiagen). DNA concentration was measured on the Nanodrop ...
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bioRxiv - Molecular Biology 2023Quote: ... on a Retsch MM400 mixer mill for 60 seconds at 30 Hz and DNA was extracted using the DNeasy Plant Mini kit (Qiagen). DNA was eluted in 100 µL of elution buffer AE (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... Each adhesive cap at an density of approximately 13–60 cells was independently processed for DNA extraction using a QIAamp DNA micro kit (Qiagen). This approach allowed enough DNA input material to prepare sequencing libraries and detect variants unique to each zygote ...
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bioRxiv - Genomics 2019Quote: ... genomic DNA and protein using AllPrep DNA/RNA/Protein Mini Kit according to the manufacturer’s instructions (Qiagen). Stable and Dox-inducible expression cell line was generated by transfecting the indicated PiggyBac vectors in the presence of hyperactive transposase plasmid (hyPBase ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA and proteins were recovered by using the Allprep DNA/RNA/Protein Mini Kit (Qiagen 80004). The efficiency of Cre-induced recombination in Appflox/flox mice was verified by PCR with primers F ...
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bioRxiv - Neuroscience 2020Quote: ... Protein and RNA extraction was done using Qiagen AllPrep DNA/RNA/Protein isolation kit (Qiagen Inc, USA). RNA purity was confirmed with 260/280 OD ratio using Nanodrop reader (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... some samples were lysed for total protein extraction using Qproteome Mammalian Protein Prep Kit (Qiagen, Hilden, Germany). Western blot was done using capillary electrophoresis based system Wes™ (ProteinSimple ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... RNA was extracted using the Allprep RNA/Protein Kit (Qiagen). The quality and quantity of RNA were assessed using the Experion RNA Analysis Kit (BioRad ...
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bioRxiv - Cancer Biology 2020Quote: ... or the AllPrep DNA/RNA/Protein mini kit (Qiagen #80004) and quantified using 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: Protein phosphorylation assay was performed using PhosphoProtein Purification Kit (Qiagen), exactly as described by manufacture’s guideline ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and extracted using AllPrep DNA/RNA/Protein Mini kit (Qiagen), as previously described102.
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
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bioRxiv - Cell Biology 2021Quote: ... and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed in triplicate.
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Total mouse RNA was isolated using RNeasy mini kit (Qiagen) and reverse transcribed to cDNA using an oligo (dT ...
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bioRxiv - Immunology 2022Quote: ... or from frozen mouse BMDMs using RNeasy Mini kit (Qiagen). Reverse transcription (RT ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA quality was assessed on a PicoRNA Bioanalyzer 2100 chip and underwent ribosomal RNA depletion using QIAseq FastSelect (Qiagen). Sequencing libraries were constructed using the NEBNext II directional RNA library kit for Illumina sequencing (NEB ...
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bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins from HGCC cells and GBM tissue were extracted with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Neuroscience 2019Quote: ... RNA and protein were extracted for ECM composition analysis using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was processed with the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from pools of 60 whole adult males and 15 whole virgin adult females using DNeasy Blood and Tissue kit (Qiagen, CA) and Promega DNA Clean and Prep Kit (Promega ...
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bioRxiv - Cell Biology 2020Quote: Isolated Chromatin was digested with 1% proteinase K for 2 hrs at 60 °C with 300 rpm and was subsequently purified by using QIAquick® Nucleotide Removal Kit (28306, Qiagen) according to the manufactory’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen, Cat #28206). Library preparation was performed on purified fragments using Nextera DNA Flex Library Prep Kit (Cat #20018704 ...
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bioRxiv - Immunology 2020Quote: ... A minimum of 60 ng bisulfite-treated (EpiTect; Qiagen) genomic DNA was used for qRT-PCR to quantify ∑ TSDR demethylation ...
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bioRxiv - Microbiology 2020Quote: ... 60 µl of Proteinase K (Qiagen, Valencia, California, USA), and two stainless-steel beads (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and eluted in 60 μL of buffer AVE (QIAGEN). RNA ligations were diluted tenfold and used for reverse transcription without extraction ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... 60 µl of 20 mg/ml Proteinase K (Qiagen) was added and the sample was incubated at 55 °C overnight ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... 60 µl of 20 mg/ml RNase A (Qiagen) was added and mixed ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA was extracted with DNA/RNA/Protein Mini Kit (Qiagen). The quality and quantity of RNA was assessed using the Agilent RNA 6000 Nano Kit Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... and AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen). Isolated DNA and RNA samples were quantified by Nanodrop (ThermoFischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mouse and viral DNA were isolated from mouse tissue using the Qiagen DNeasy Blood and Tissue Kit (Qiagen). iTAQ universal Syber Green supermix (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA and DNA was extracted from the same cells from neuropathologist annotated tumour regions (>60% cancer cells) using appropriate Qiagen kits (Qiagen, Sussex, UK). Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... region was amplified using fungal-specific primers (60): ITS1F (5’-CTTGGTCATTTAGAGGAAGTAA-3’) and ITS4 (5’-TCCTCCGCTTATTGATATGC-3’) and the HotStarTaq Plus Master Kit (Qiagen, Valencia, CA). Amplicons from different samples were pooled to equimolar concentrations and purified of short fragments using Agencourt Ampure beads (Agencourt Bioscience Corporation ...
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bioRxiv - Microbiology 2022Quote: Fecal samples were collected from the Duroc piglets at 60□±□8 days of age and microbial DNA was extracted with the DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany). Extracted DNA was sent to the University of Illinois Keck Center for paired-end (2□×□250 bp ...
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bioRxiv - Microbiology 2024Quote: ... DNA was precipitated with 100% ethanol and 3 M sodium acetate at -80°C for 60 minutes and then cleaned using the Qiagen QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA) per manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Total DNA was isolated from the dried feces (one fecal sample was ca. 60 mg) using a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and purified using a Geneclean Spin Kit (MP-Biomedicals ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lines and mouse tails using DNeasy Blood & Tissue kit (Qiagen). Exome libraries were prepared by the Advanced Sequencing Facility at the Crick and sequenced using an Illumina HiSeq 4000 using 100 base pair paired-end reads ...
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bioRxiv - Physiology 2020Quote: RNA was isolated from mouse liver using the RNeasy Kit (Qiagen). RNA degradation was monitored using a BioAnalyzer (Agilent) ...
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bioRxiv - Immunology 2020Quote: ... from mouse plasma using the QIAamp MinElute Virus Spin Kit (Qiagen) along with a DNaseI (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from mouse tissue using the RNAeasy kit (Qiagen). cDNA was then immediately generated using the high-capacity cDNA reverse transcription kit (Applied Biosystems).
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bioRxiv - Bioengineering 2023Quote: Mouse RNAs were purified using the Rneasy Plus Mini Kit (Qiagen) and subsequently treated with DNase ...
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bioRxiv - Biophysics 2022Quote: ... The resulting mixture was rotated at 4 °C for 60 min and then poured into a 1 mL polypropylene column (Qiagen). The resin was washed three times with 5 mL lysis/wash buffer and eluted in 0.25 mL fractions with Ni-NTA elution buffer (composition same as lysis/wash buffer ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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bioRxiv - Genetics 2019Quote: ... and mouse anti-Strep (Qiagen Cat# 34850, 1:500) with goat or donkey secondary antibodies from Jackson ImmunoResearch used 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse antibodies against the His-tag (1:1000, Qiagen), rabbit antibodies against the C-terminal region of the collagen VI α1 chain (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).