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Citations for Qiagen :
1 - 50 of 2774 citations for Microtubule Associated Protein 1 Light Chain 3 Gamma MAP1LC3C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... plasmids bearing chimeric antibody heavy and light chain genes were prepared (EndoFree Plasmid Mega prep Kit, QIAGEN) and transfected into Human Embryonic Kidney cells (HEK293F ...
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bioRxiv - Cell Biology 2022Quote: ... Purification of His6-light chain TeTx was done using Ni-NTA resin (Qiagen) in 50 mM Tris HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... and associated protein network by using Ingenuity® Pathway Analysis (IPA) software (QIAGEN Inc.), relying on IPA’s proprietary algorithm to evaluate and minimize sample source bias ...
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bioRxiv - Immunology 2020Quote: ... cDNA products were then used in nested PCR for heavy/light chain variable region amplification with HotStarTaq Plus DNA Polymerase (QIAGEN # 203643) and specific primer sets described previously 34,35 ...
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bioRxiv - Immunology 2019Quote: ... Input material (total cytoplasmic mRNA) and efficiently translated mRNA (heavy polysome-associated, >3 ribosomes) were extracted with QIAzol (Qiagen) and purified using RNeasy MinElute Cleanup Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... We then amplified heavy and light chain sequences from the WTA products utilizing pools of partially degenerate pools of V region specific primers (Qiagen HotStar Taq Plus). Heavy and light chain amplifications were carried out separately ...
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bioRxiv - Microbiology 2021Quote: ... and associated nucleic acid extraction (Qiagen, Germany) kits were used ...
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bioRxiv - Cancer Biology 2021Quote: ... a cancer associated fibroblast (CAF) cell line (pCAF2) expressing TGF-β responsive SMAD2/3/4 RE-Luciferase (Qiagen, #CLS-017L) was created ...
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bioRxiv - Microbiology 2021Quote: ... Cln1-His6 and its associated proteins were co-purified following the Ni-NTA Spin Kit procedure (Qiagen, Valencia, CA). Cell lysate from the CLN1-His6 strain was used as a negative control ...
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bioRxiv - Microbiology 2019Quote: ... and cell-associated HSV-1 DNA was isolated with the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Microbiology 2021Quote: ... and its associated RNase- Free DNase digestion set (QIAGEN), following the manufacturer’s protocol for mammalian cells ...
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bioRxiv - Physiology 2022Quote: Powdered heart tissues (25 mg) were homogenized in 0.25 mL microtubule stabilization buffer (Supp. Table 1) with a bead mill (Qiagen, TissueLyserII). Homogenates were centrifuged at 1,000×g for 5 mins at 37 ℃ ...
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bioRxiv - Genomics 2021Quote: ... Chromatin-associated RNA was isolated with RNeasy Mini Kit (QIAGEN 74104) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and analyzed using the PyroMark Q24 machine and associated software (Qiagen). Graphical representation and statistical analysis was performed with GraphPad Prism software ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin-associated RNA was extracted using Qiazol lysis reagent (Qiagen, 79306) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... cancer-associated fibroblasts (CAFs) and C666-1 cells using RNeasy Mini kit per the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA integrity number (RIN ...
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bioRxiv - Microbiology 2020Quote: ... adareanum-associated microbial cell preparations were extracted with Powerlyzer DNEasy extraction (Qiagen) following manufacturer's instructions starting at with the addition of the lysis solution ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Genomics 2022Quote: ... Age-associated regions were amplified using the PyroMark PCR Kit (Qiagen, Hilden, Germany) with manufactures instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Genomic DNAs associated with beads were recovered using a QIAquick PCR Purification Kit (Qiagen) and then used to quantify their amounts by qPCR.
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP10-1 (5’-UCGCUUUGGAUGGAAGUUCUA-3’, Qiagen), si-USP10-2 (5’-UACGUCAACACCCAUGAUAGA-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from leaf samples of the control and treated (chilling, high light and chilling plus high light) plants using the RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP36-1 (5’-CAAGAGCGUCUCGGACACCUA-3’; Qiagen, Microsynth), si-USP36-2 (5’-UCCGUAUAUGUCCCAGAAUAA-3’ ...
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bioRxiv - Microbiology 2023Quote: ... Polymerase chain reaction (PCR) was performed with the HotStarTaq Plus polymerase (Qiagen) with 5 min initial denaturation at 94°C ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of RGS(His)6-tagged 14-3-3 was performed by applying the anti-RGS(His)6 antibody (Qiagen) in combination with a secondary anti-mouse HRP antibody.
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bioRxiv - Microbiology 2021Quote: Recombinant protein was blotted with anti-His antibody using the same method: 1:2000 mouse anti-tetra-His IgG (Qiagen); 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Immunology 2021Quote: 1-3 × 105 PBMCs were lysed in QIAzol (Qiagen). Full-length cDNA was then synthesized using the SMARTer technology (Takara Bio) ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reaction (PCR) was performed using Taq DNA Polymerase (QIAGEN, Hilden, Germany). The fungal ITS-28S rRNA gene region was obtained using the ITS5 (White et al. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... QuantiTect Probe RT-PCR (reverse transcription polymerase chain reaction) Kit (QIAGEN, Hilden, Germany) was used to perform RRT-PCR reactions in the ABI 7300 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: Polymerase Chain Reactions (PCRs) were performed using Type-it Microsatellite PCR Kit (Qiagen) in 10 µl reactions ...
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bioRxiv - Neuroscience 2021Quote: ... then associated RNAs were lysed into RLT buffer and RNA was isolated using the RNAeasy Micro kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Gene expression changes associated with osteogenesis were assessed using an RT2 Profiler™ PCR Array (PAMM-026Z, Qiagen). A fold regulation threshold of 2 was used to determine up- or down-regulation of each gene in male and female PAE mice compared to control and data is presented as log2 fold change ...
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bioRxiv - Cancer Biology 2021Quote: Differential expression of cell cycle-associated genes was determined using the EMT RT2 profiler qPCR array (Qiagen, PAHS-020Z) and analyzed using the Qiagen online Data Analysis Tool ...
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bioRxiv - Neuroscience 2023Quote: ... and regulatory network interactions associated with differentially expressed genes were identified using QIAGEN Ingenuity Pathway Analysis (IPA) (QIAGEN Inc.). Genes with absolute fold change greater than 1.5 were included and molecules and relationships were filtered for “tissues/cell lines=amygdala ...
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bioRxiv - Physiology 2023Quote: ... and real-time polymerase chain reaction (PCR) was performed with Fast SYBR Green (QIAGEN). Gapdh was used as the housekeeping gene ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... Thermal cycling was performed on the Rotor-Gene Q real-time PCR cycler and data were analyzed with the associated software (Qiagen) using a cycle threshold of 0.03 ...
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bioRxiv - Molecular Biology 2020Quote: Functional annotation of genes that are associated with dynamic chromatin regions and/or differential expression with NSC activation was performed using Ingenuity Pathway Analysis (Qiagen Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... 350 µl of 100% ethanol was added to the tube (“TRAP” fraction: enriched in transcriptome associated to EGFP-tagged ribosomes) and then loaded onto a RNeasy MinElute column (Qiagen). RNA was isolated using RNeasy Mini Kit (#74104 ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA was extracted as described in the associated method below or by using the RNeasy RNA mini kit (Qiagen). Then ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
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bioRxiv - Molecular Biology 2021Quote: Polymerase chain reaction (PCR) products were gel purified using Qiagen QlAquick gel purification columns (Qiagen) according to the manufacturer’s instructions ...