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Citations for Qiagen :
101 - 150 of 767 citations for Macrophage metalloelastase MMP12 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies (α-His HRP conjugated (Qiagen 34460) or α -VSV-G (Millipore sigma V4888-200UG) ...
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bioRxiv - Neuroscience 2024Quote: ... 6 µl Hi-Perfect transfection reagent (Qiagen, 301707), and 1 µl of the desired (20 µM ...
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bioRxiv - Neuroscience 2024Quote: ... 7.5 µl Hi-Perfect transfection reagent (Qiagen, 301707), 1.25 µl siRNA stock (20 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 µl Hi-PerFect Transfection Reagent (Qiagen; #301705) and 60 µl of siRNA mixture (1 µM ...
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bioRxiv - Biophysics 2024Quote: ... or anti-His6X (His HRP conjugate by Qiagen) antibodies at a 1:1,000 dilution ...
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bioRxiv - Biochemistry 2021Quote: Messenger RNA was extracted from HEK293 cells using an RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and used as the template to synthesise complementary DNA (cDNA ...
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bioRxiv - Biophysics 2024Quote: The HEK293 stable cell lines were generate by a transfection with Effectene (Qiagen) and 1µg plasmid (pmCherry-N1-hERG-WT and -A561V ...
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bioRxiv - Neuroscience 2020Quote: ... and isolated microglia/macrophage (CD11b+ cells) using RNeasy plus Mini kit (Qiagen, 74134). cDNA synthesis was performed using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... macrophages were washed with PBS and RNA was isolated using RNeasy kit (Qiagen). For transcriptomic analysis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The concentration of the resulting His-tagged ß-CATENIN-1 fragments was quantified via quantitative Western blotting with a Penta-His antibody (Qiagen) and a recombinant His-tagged protein as external standard.
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Cancer Biology 2019Quote: ... His-tagged proteins were purified on NiNTA beads (Qiagen). Purified proteins were eluted with 500 mM NaCl ...
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bioRxiv - Genetics 2019Quote: ... or anti-tetra His (0.1 μg/ml Qiagen 34670) were used as primary antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His is a peroxidase-conjugated antibody (Qiagen). The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... PABPC1-His was bound to Ni-NTA resin (Qiagen), washed by 1x PBS (10 mM imidazole added ...
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bioRxiv - Microbiology 2020Quote: ... The anti-His antibody conjugated to horseradish peroxidase (Qiagen) was used at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2021Quote: ... 1:1,000 dilution of α-His-HRP antiserum (Qiagen), or 1:1,000 dilution of α-OmpA antiserum (Antibody Research Corporation ...
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bioRxiv - Plant Biology 2022Quote: ... and NoFa-ICL was the Penta-His antibody (Qiagen) at 1:4000 in TBST ...
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bioRxiv - Immunology 2022Quote: ... then incubated with the primary antibody anti-His (Qiagen) at a concentration of 1:2500 in a solution of 3% non-fat milk in TBS-T overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Anti-Penta-His antibody was purchased from Qiagen (Germany).
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Immunology 2020Quote: Total RNA of IFN-α2 treated HEK293 cells was purified using RNeasy columns (Qiagen) with on-column DNase I digestion ...
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bioRxiv - Microbiology 2020Quote: ... 30 nM of total siRNA were transfected into macrophages using HiPerFect transfection reagent (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: RNA was directly extracted from macrophages using the RNeasy Plus Mini kit (Qiagen, 74034) and cDNA synthesized using SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... ∼1000 macrophages were sorted into 75µL of RLT buffer (Qiagen, containing 1% beta-mercaptoethanol), vortexed for 1 min and immediately frozen (–80°C) ...
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bioRxiv - Microbiology 2024Quote: Total RNAs from Leptospira species or macrophages were extracted using QIAzol lysis reagent (Qiagen) and purified with RNeasy columns (Qiagen) ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from hepatic macrophages using the RNeasy Plus Mini kit (Qiagen). Samples were sequenced on a Novaseq 6000 using a 150 PE flow cell at the University of Minnesota Genomics Center ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were transfected with hTRPM3α2-GFP or its mutants using the Effectene reagent (Qiagen). Cells were loaded with 1 μM fura-2 AM (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... His-tagged proteins were purified over Ni-NTA-agarose (Qiagen) with 3 wash steps at 30 mM imidazole and elution with 250 mM imidazole in denaturing buffer ...
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bioRxiv - Microbiology 2019Quote: ... A 10 nM solution of biotinylated penta-His antibody (Qiagen) was incubated for 10 min on the neutravidin-coated surface and excess unbound antibody removed (22 ...
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bioRxiv - Bioengineering 2020Quote: ... Anti-Penta-His Alexa Fluor 488 conjugate was from Qiagen.
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bioRxiv - Microbiology 2021Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (QIAGEN) and were eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Microbiology 2022Quote: ... and His-PutA was purified with Ni-column chromatography (Qiagen). The purified His-PutA protein (50 μg ...
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bioRxiv - Biochemistry 2021Quote: Penta-His HRP conjugate was purchased from QIAGEN (Hilden, Germany). Anti-BepA (Narita et al. ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified with Ni2+-NTA beads (Qiagen) and eluted with 300 mM imidazole in PBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were bound by Ni-NTA agarose (Qiagen) and eluted with 500 mM imidazole in HSB ...
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bioRxiv - Molecular Biology 2024Quote: ... His-tagged protein was bound to Ni-NTA beads (Qiagen). The beads were washed with 100 mL lysis buffer and protein was eluted with 40 mL elution buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: PerC macrophages obtained from WT or Sparc-/- mice were extracted using RNAeasy micro kit (Qiagen). After quality check and quantification by 2100 Bioanalyzer system (Agilent ...
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bioRxiv - Cell Biology 2024Quote: Total RNA of ECs and liver macrophages was isolated with RNeasy plus Mini Kit (Qiagen). Total RNA of the liver tissues was extracted with Trizol (Thermo) ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Sorted Lp and Ln macrophages were lysed with lysis buffer (RNeasy Mini Kit, Qiagen, Cat# 74104) and stored at -80°C ...
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from monocytes and macrophages using AllPrep DNA/RNA Mini Kit (Qiagen, cat# 80204) and treated with RNAse-free DNAse (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA (n=3/experimental group) was extracted from macrophages with RNeasy Mini Kit (Qiagen, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA from macrophages or primary tumors was extracted using an RNeasy Mini kit (#74104, Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Total RNA was extracted from peritoneal macrophages using the RNeasy mini kit (Qiagen, Valencia, CA, USA) and first-strand cDNA was synthesized from the total RNA (250 ng ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: Macrophages were lysed and total RNA was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands). Reverse transcription of cDNA was performed with the High-Capacity cDNA Reverse Transcription kit (Life Technologies ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from sorted CD11bhiF4/80hi peritoneal macrophages using the miRNeasy Mini Kit (QIAGEN). Purity of sorted macrophages were > 99% ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: RNA was isolated from macrophages using the Qiagen AllPrep RNA/DNA Micro Kit (Qiagen, Germantown, MD). The macrophage global pattern of gene expression was determined using RNASeq on the Illumina platform.8 Reads were quantified by kallisto using Gencode v24 as the reference genome ...