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Citations for Qiagen :
451 - 500 of 615 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Fecal samples positive for the partial E gene of sarbecovirus were homogenized in TissueLyser II (Qiagen) using 0.1 mm glass beads (Tomy Seiko ...
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bioRxiv - Biochemistry 2020Quote: ... Released affinity tags containing Flag-EGFP were removed from the mixture by a Ni-NTA resin (QIAGEN). The non-absorbed fractions were concentrated and subjected to a size-exclusion column chromatograph using a Superose6 Increase column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were amplified in Escherichia coli DH5a and purified using HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Microbiology 2020Quote: ... coli C600 strain using an Endofree Maxi-Prep kit from Qiagen (QIAGEN, catalog number 12362) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... induced in BL21-CodonPlus(DE3)-RIL Escherichia coli and affinity purified with Ni2+ agarose (Qiagen). The purified His6Tam41 protein was used as an antigen in rabbits (Pacific Immunology) ...
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bioRxiv - Microbiology 2021Quote: ... coli using a QIAprep Spin Miniprep Kit according to manufacturer’s instructions (Qiagen, Germany, Hilden, Germany). The phage promoter eGFP expression cassette (pCMP1::eGFP ...
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bioRxiv - Molecular Biology 2022Quote: ... coli following standard procedures and purified by using the Qiagen Miniprep Kit (Qiagen, Maryland, USA). All sequences and constructs were checked by sequencing before further use ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... coli and then extracted and purified using a plasmid purification maxi kit (Qiagen, Valencia, CA). Purity was assessed by the ratio of absorbance at 260 and 280 nm ...
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bioRxiv - Cell Biology 2019Quote: ... coli BL21(DE3) and purified on Ni-NTA-agarose as described by the manufacturer (Qiagen). Before elution an additional washing step with ATP was performed.
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bioRxiv - Plant Biology 2020Quote: ... coli was used for cDNA synthesis employing QuantiTect® Reverse Transcriptase Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... coli lysates under native conditions by immobilised metal affinity chromatography using Ni-NTA agarose (Qiagen) essentially as described 12 using 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... coli DH5α cells and isolated through the QIAprep Spin Miniprep Kit (Qiagen; Cat. No. 27106). All the cloned plasmids (Supplementary Table S3 ...
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bioRxiv - Biochemistry 2022Quote: ... coli lysates under native conditions as described in the fifth edition of the QIAexpressionist (Qiagen). The lysates were loaded onto a 1 ml HisTrap HP column (GE healthcare ...
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bioRxiv - Immunology 2023Quote: ... The PCR fragments were ligated into an Escherichia coli expression vector (pQE30; Qiagen, Hilden, Germany) to create a protein construct with a six-tandem histidine residue (His-tag ...
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bioRxiv - Microbiology 2023Quote: ... coli-specific adaptations of the RNA extraction protocols in the RNAProtect Bacteria Reagent Handbook (QIAGEN). Following RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... coli following ampicillin selection using the QIAGEN Plasmid Purification Midi-Prep Kit (QIAGEN, Hilden, Germany) and reconstituted in sterile Tris-EDTA (TE ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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SARS-CoV-2 comprehensive receptor profiling: mechanistic insight to drive new therapeutic strategiesbioRxiv - Cell Biology 2021Quote: ... each pre-incubated at a 2:1 molar ratio with Penta His Alexa Fluor 647 Conjugate (Qiagen, UK). Hits (duplicate AF647 positive spots ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Microbiology 2020Quote: A 6.5 nM concentration of His-tagged 16055 SOSIP.v8.3 trimer in TBS was added to a 96-well Ni-NTA plate (Qiagen) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... His-SFP1 was purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen). Recombinant His-SFP1 was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Microbiology 2023Quote: ... The N-terminal His-tagged Sif protein (Lmo0946-His6) was expressed and purified using Ni-NTA resin (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged talin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a gel filtration column (Superdex 200 ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates of His-tagged vinculin constructs were purified by Ni-NTA-sepharose affinity chromatography (Ni2+-nitrilotriacetic acid, Qiagen), followed by a Q-Sepharose ion exchange column ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The agarose gel bound with the His-tagged recombinant protein was packed in the chromatography column (Qiagen, Germany), and the flow through was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... All fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using anti-His (Qiagen) and anti-strep (MilliporeSigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were washed with BRB80 and then were coated with mouse antibody against 6xHis-tag (1:50; Qiagen) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... IL2-Rα ectodomain was produced with C-terminal 6xHis tag and purified on Nickel-NTA spin columns (Qiagen) according to manufacturer instructions.
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Bioengineering 2020Quote: ... coli bacteria (as described in section 2.1) and extracted using a Plasmid Maxi kit (Qiagen, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli isolate using a modified version of the Qiagen DNeasy PowerLyzer microbial kit (Qiagen, Hilden, Germany). Using a 10μl loop ...
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bioRxiv - Plant Biology 2024Quote: ... coli under a T7 promoter and purified using Ni-NTA nickel affinity beads (Qiagen, Hilden Germany). Purified protein was dissolved in PBS solution and lyophilized ...
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bioRxiv - Biophysics 2021Quote: ... gliding motility assays were performed with specific immobilization of motors on the surface via penta-His antibodies (34660, Qiagen) as previously described[64] (Figure S4) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Molecular Biology 2022Quote: ... or Renilla-HA-NOD2 (750 ng/well) and His-FLAG-CAD (250 ng/well) using Effectene Transfection Reagent (Qiagen). The following day ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP ± MirA was loaded onto a column with 80 µL of previously equilibrated Ni-NTA resin (Qiagen) and incubated for 1 h at 4°C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Biochemistry 2021Quote: ... was transformed with an N-terminally His-tagged fusion protein of cytHPPK/DHPS (At1g69190) in the vector pQE30 (Qiagen). Overnight cultures grown at 30°C in LB broth supplemented with 35 µg/mL kanamycin and 100 µg/mL ampicillin were sub-cultured to an OD600 of 0.6 - 0.7 and induced overnight at 16°C with 100 mM isopropyl β-D-1-thiogalactopyranoside ...