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Citations for Qiagen :
101 - 150 of 851 citations for Lassa Fever Virus GP1 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RNA was isolated from 200 μL sample using the QIAamp MinElute Virus spin kit (Qiagen, Frederick, MD). For the qRT-PCR assay ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The RNA from the escaped virus was isolated using a QIAamp viral RNA mini kit (Qiagen, #52904), and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: Total virus-RNAs were extracted from SK.N.SH infected cells at MOI 0.5 using RNeasy minikit (Qiagen, #74104). ISRE reporter assay was performed in STING37 cells plated in 24 wells plate (2×105 cells/well ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from the concentrated virus with the QIAamp Viral RNA Mini Kit (Qiagen, 52904). The sgRNA sequences found in the genomic DNA and viral RNA samples were amplified by PCR (Agilent ...
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bioRxiv - Genomics 2022Quote: Viral RNA was extracted from 200 μl of clinical sample using QIAamp 96 Virus QIAcube HT (QIAGEN) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... Viral RNA (vRNA) was isolated from blood plasma using the Qiagen QIAmp UltraSense Virus Kit (Qiagen #53704) in accordance with manufacturer’s instructions for 0.5 mL of plasma ...
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bioRxiv - Microbiology 2023Quote: ... and cleared supernatants stored at −80°C until virus titration/ spin through a QIA shredder column (Qiagen). Viral titers were expressed as TCID50 units per gram source tissue.
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bioRxiv - Microbiology 2021Quote: ... Isolates were cultured overnight on sheep blood agar and plasmid DNA was extracted using Qiagen Plasmid Midi kit (Qiagen Inc, Germantown, MD) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acids were extracted using QIAamp 96 virus Qiacube HT kit on QIAxtractor Automated extraction (Qiagen, US) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: The virus RNA was extracted from clinical samples using the QIAamp viral RNA mini kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: Viral RNA from cell culture supernatants was isolated as the manufacturer’s instructions using QIAamp MinElute Virus kit (Qiagen). Cells were suspended in TRI Reagent (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: Load Viral RNA was isolated from plasma and cerebral spinal fluid (CSF) using the QuantiTech Virus Kit (Qiagen). Viral loads were quantified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was extracted from 200 µl of the inactivated supernatant using the EZ1 Virus Mini Kit (Qiagen, Germany). As described previously ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from nasopharyngeal swab samples on the QiaSymphony platform using the Virus Pathogen Mini Kit (Qiagen). Libraries were prepared using the Illumina DNA Flex library preparation kit and sequenced on an Illumina MiSeq (V2 ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from 140 µl of virus stock (SARS-CoV-2 VOC Delta, GISAID ID: EPI_ISL_15250227) using a QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: RNA from virus stocks was extracted with the QIAamp Viral RNA Mini Kit (Cat# 52904, QIAGEN, Hilden, Germany), and one-step cDNA synthesis was then performed with SuperScript IV RT Viral cDNA (Cat# 12594025 ...
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bioRxiv - Immunology 2022Quote: ... 200 μL of nasal swab sample was extracted with the QIAamp 96 Virus QIAcube HT kit (Qiagen, Germany) on the QIAcube HT System (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was extracted from the virus stocks using the QIAamp viral RNA mini kit (Qiagen, Cat#74l36) and viral genomes were sequenced as described before [31].
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bioRxiv - Microbiology 2023Quote: Virus isolates and respiratory specimens were extracted using the QIAgen Viral RNA Mini-Kit (QIAgen, Inc., Valencia, CA). Input sample volume was 140 μl ...
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bioRxiv - Microbiology 2020Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Epidemiology 2019Quote: Total RNA was extracted from SFTS virus IgM positive samples using a QIAamp viral RNA mini Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Viral RNA (vRNA) was isolated from virus-infected lung homogenate supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN). Influenza genome copies were quantified from RNA isolations by reverse transcription and quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction of viral nucleic acids using a commercial kit (QIAamp MinElute virus spin kit, Qiagen, Venlo, Netherlands). A portion (2.5 µl ...
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bioRxiv - Microbiology 2021Quote: ... the resulting filtrate was used for viral DNA extraction using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Nucleic acid was first extracted from each blood sample using QIAamp MinElute Virus Spin kits (Qiagen, Mississauga, Ontario, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Purified ZIKV RNA and HSV-2 DNA were extracted using QIAamp MinElute Virus Spin Kit (Qiagen, catalog no. 57704), and 50ng purified viral RNA/DNA was incubated with the recombinant protein at different concentrations for 90min at 37°C in the presence of SUPERase In™ RNase inhibitor (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently extracted using the Qiagen EZ1 Advance XL extractor with the EZ1 Virus Mini Kit v2.0 (Qiagen, Germany). For the eastern Australian RSV cases ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10,000 TCID50 WT and NS1R38A virus containing inoculum using the QIAamp Viral RNA mini kit (Qiagen), according to the manufactures protocol ...
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bioRxiv - Immunology 2021Quote: ... viral RNA was extracted from AFs stocks using the QIAamp MinElute Virus Spin kit (cat. n° 57704, Qiagen, Germany), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fold changes of N transcript expression with each treatment were calculated corcenning to the Virus control using REST 2009 program (v2.0.13, Qiagen, GbmH) [26] ...
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bioRxiv - Microbiology 2022Quote: ... Sample DNA was extracted from the viral enriched filtrate using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) and was subjected to whole genome amplification (WGA ...
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bioRxiv - Microbiology 2022Quote: ... followed by total nucleic acid extraction of 400 ul of pretreated stool using the EZ1 Virus Mini Kit v2.0 (Qiagen). Extracts were eluted in 60 ul volume.
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bioRxiv - Immunology 2022Quote: Serum viral RNA was extracted from 200 μl using the QiaAmp Min Elute Virus Spin kit (Qiagen, Cat# 57704) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were further processed on the QIAsymphony platform using the QS DSP Virus/Pathogen Midi Kit (Qiagen, Hilden, GER) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2022Quote: Viral genomic RNA was purified from 280 μL virus-containing media using the QIAamp Viral RNA Mini Kit (Qiagen) as per the manufacturer’s instructions and eluted in 60 μL nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: We extracted influenza virus genomic RNA from the clarified allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA from one aliquot per condition per virus isolate and negative control was immediately extracted with the QIAamp Viral RNA Mini Kit (Qiagen) and stored at −80°C until further processing ...
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bioRxiv - Microbiology 2021Quote: Total RNA of each isolate was extracted from frozen virus stock using QIAamp Viral RNA Mini Kit (Qiagen, Germantown, MD) following the manufacturers spin protocol ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the working stock of each reporter virus and treated with DNase using the DNase Max kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: 200µL of lung clarified homogenate or infectious cell supernatant (virus stock) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 70 μL of supernatant using QIAamp 96 virus QIAcube HT kit on the QIAcube HT System (Qiagen). Each RNA sample was analyzed by one-step RT-qPCR with two SYBR Green assays ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from supernatants of swab material using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). Autopsy tissues were collected from lung ...
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bioRxiv - Microbiology 2019Quote: For viral genome copy measurements RNA was extracted from 2 µl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from supernatants of hamster nasal wash samples using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Viral RNA extraction was performed on an EZ1 Advanced XL device using the EZ1 virus mini kit V2.0 according to the manufacturer’s recommendations (Qiagen, Courtaboeuf, France). The qPCR was run on a Lightcycler® 480 thermocycler (Roche diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...