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Citations for Qiagen :
201 - 250 of 406 citations for L Phenylalanine N T Boc 13C9 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... DNA was isolated from sorted IFNγ+ and IFNγ− CD8+ T cells using the All-Prep DNA/RNA Micro Kit from Qiagen and sent to Adaptive Biotechnologies for survey-level TCRβ sequencing ...
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bioRxiv - Immunology 2021Quote: DNA was extracted from flow cytometry-sorted T cells using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and mean TPM value between uninjured tails and tail blastemas were compared with a two-tailed t-test using the CLC genomic workbench with default parameters (Qiagen). A transcript or HHGC was deemed differentially expressed when its fold-change is greater than 2 or less than −2 and the FDR adjusted P-value < 0.05 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were subsequently cloned into pGEM®-T Easy Vector plasmids in a JM109 High- Efficiency Competent Cells (Qiagen) strain of Escherichia coli ...
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bioRxiv - Immunology 2022Quote: ... mRNA was extracted from purified T-lymphocytes by silica-membrane spin columns through use of RNAeasy mini kit (#74104, Qiagen), then immediately converted to cDNA by High-Capacity cDNA Reverse Transcription Kit (#4374966 ...
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bioRxiv - Immunology 2019Quote: ... genomic DNA purified from CD8 T cells and bisulfite converted using the EpiTect Bisulfite Kit as per the manufacturer’s instructions (Qiagen, Inc.). Bisulfite-converted DNA was PCR amplified and cloned with the TOPO TA cloning kit (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Brain and DRG samples from up to four donors per species were homogenized in Tissue Protein Extraction Reagent (T-PER) supplemented with HALT protease inhibitor and benzonase endonuclease using a Tissue Lyser Mixer Mill Grinder (Qiagen), while serum and CSF were directly diluted in supplemented T-PER ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from freshly isolated T-cells on day 7 of treatment from spleens using RNeasy Kit (Qiagen). For each group ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from CD3+ T-cells using the Qiagen RNeasy Plus Mini Kit or EN AllPrep Mini Kit with on column DNaseI (Qiagen) digestion ...
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bioRxiv - Immunology 2020Quote: ... was isolated from previously sorted T cells and monocytes (CD19-CD3+/CD14+)30 using the QiaAmp DNA mini kit (Qiagen), and the concentration was measured on Nanodrop.
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the large stocks of recombinant T/F LTR-pGL3 reporter plasmids were generated using Plasmid Maxi kit (Qiagen, Valencia, CA) for immediate use or longer storage at -80 °C.
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from 1-2*106 sorted naïve (CD25− CD44lowCD62high) or effector (CD25− CD44highCD62low) CD4+ T-cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Immunology 2023Quote: Real-time quantitative PCR (RT-qPCR) was done as previously described [Zonneveld 2021] using the Human T cell Tolerance & Anergy RT2 Profiler PCR array (Qiagen). The relative expression levels of each gene were normalized using 4 reference genes (B2M ...
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bioRxiv - Cell Biology 2023Quote: ... significantly regulated human proteins (t-test, p-value ≤ 0.01; described above) were characterized by core analysis in IPA® software (version 68752261, QIAGEN Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... deep sequencing genomic DNA was isolated from PBMC or CD4+ and CD8+ sorted T cell populations following the DNeasy Blood and Tissue Kit (Qiagen) protocol ...
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bioRxiv - Immunology 2023Quote: ... Immune cells were FACS-sorted for APC-enriched samples or T cell-enriched samples and RNA was subsequently extracted (RNeasy Mini Kit, Qiagen). RNA was analyzed on an nCounter MAX Analysis System (Nanostring ...
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bioRxiv - Immunology 2023Quote: Genomic DNA from ex vivo isolated CD8+ T cell subsets or in vitro cultured cells was extracted using the DNeasy Blood & Tissue Kits (Qiagen) and subsequently bisulfite-converted by using the EZ DNA Methylation-Lightning Kit (Zymo Research ...
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bioRxiv - Pathology 2023Quote: ... RNA from CD4+ T cells (2×106 cells per condition) was extracted by using the RNeasy Plus Mini kit (QIAGEN). Extracted total RNA was quantified using the Qubit broad range RNA assay ...
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bioRxiv - Immunology 2024Quote: ... T-cell pellets were resuspended in 350 µL of RLT+ buffer from the Qiagen RNeasy plus mini kit (Qiagen, 74136) and RNA was extracted according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: TET2 knockout efficiency was confirmed by isolating genomic DNA from CAR T-cells at Day 7 using the dNeasy Blood & Tissue Kit (Qiagen). PCR of genomic DNA was performed with TET2 Forward Primer 5’-TCCCTGAGTCCCAGTCCATC-3’ and Reverse Primer 5’-TCAGGAATGGCCAGGTTCTG-3’ using MyTaq Red 2X Mix (Meridian Bioscience) ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from P15 liver tissue (n=4 biological replicates per strain) using QIAzol Lysis Reagent (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Bacterial genomic DNA was extracted from overnight (o/n) bacterial liquid cultures with DNeasy® Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-N-WASP ± MirA was loaded onto a column with 80 µL of previously equilibrated Ni-NTA resin (Qiagen) and incubated for 1 h at 4°C with agitation ...
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bioRxiv - Genomics 2021Quote: ... total RNA was isolated from patient blood samples (CD19+ presorted n=161) using the RNA RNeasy mini kit (Qiagen). RNA quantification was performed with a Qubit 2.0 Fluorometer ...
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bioRxiv - Cancer Biology 2022Quote: RNA from Nestin(N)/tv-a;Ink4a/Arf-/- neural progenitors was isolated using the RNeasy Kit (Qiagen, Catalog# 74104) and 1μg of total RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... viral RNA was extracted from AFs stocks using the QIAamp MinElute Virus Spin kit (cat. n° 57704, Qiagen, Germany), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... was transformed with an N-terminally His-tagged fusion protein of cytHPPK/DHPS (At1g69190) in the vector pQE30 (Qiagen). Overnight cultures grown at 30°C in LB broth supplemented with 35 µg/mL kanamycin and 100 µg/mL ampicillin were sub-cultured to an OD600 of 0.6 - 0.7 and induced overnight at 16°C with 100 mM isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... of vehicle and Acyl-GIP vehicle treated LDLR−/− mice (n = 5) using Qiazol according to the manufacturer’s instructions (Qiazol Lysis Reagent, QIAGEN). The quality of the RNA was determined with the Agilent 2100 BioAnalyzer (RNA 6000 Nano Kit ...
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bioRxiv - Microbiology 2022Quote: ... N-terminal His-tagged SlPR1 and SlChi3 proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA extraction of gill tissue (N = 16 individuals per population) was performed using the DNeasy Blood & Tissue Kit (Qiagen). Further purification of the extracted DNA was done with NucleoSpin® gDNA Clean-up (Macherey-Nagel) ...
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bioRxiv - Microbiology 2019Quote: Initial N-gene amplicon analysis was performed with 0.2 μl Round 2 product using the QIAxcel (Qiagen, Hilden, Germany). For positive reactions ...
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bioRxiv - Cancer Biology 2021Quote: ... Those PDTOs that underwent FOLFOX treatment (n=14) had RNA extracted using the Qiagen RNAeasy micro kit (Qiagen, # 74004).
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bioRxiv - Microbiology 2020Quote: Following 72 h post treatment with a sublethal concentration of compound 33 (3.13 µM), adult male worms (n = 20 worms, three biological replicates) were homogenized with a TissueLyser (Qiagen) and total histones extracted using the EpiQuikTM Total Histone Extraction kit (OP-0006 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected brains (n = 9 brains per experimental cohort) by RNeasy Mini Kit (Qiagen, 74104), with on-column removal of genomic DNA (Qiagen ...
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bioRxiv - Immunology 2023Quote: RNAseq was performed on total PBMCs from n=36 individuals in our cohort using the AllPrep kit as per manufacturer’s instructions (Qiagen). RNA libraries ...
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bioRxiv - Genomics 2019Quote: RNA from PDX-derived T-ALL samples was extracted using a RNeasy Mini kit according to manufacturer’s instructions (cat 74106, Qiagen, Hombrechtikon, Switzerland), and cDNA was generated using High Capacity cDNA Reverse Transcription Kit (Applied BioSystems ...
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bioRxiv - Physiology 2019Quote: ... Methylation status of the individual CpG sites was identified as an artificial C/T SNP using QCpG software (Pyrosequencing, Qiagen, UK). Then % methylation of each CpG site was then calculated as a proportion of the methylated alleles divided by the sum of all methylated and unmethylated alleles ...
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bioRxiv - Immunology 2020Quote: ... Up to 8 colonies were selected for each TCRα and TCRβ sequence for each of the KRASG12V/D-specific T cell clones and DNA was isolated using a QIAprep Spin Miniprep Kit (QIAGEN, 27104) and sequenced using the M13F and M13R primers ...
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bioRxiv - Immunology 2020Quote: ... and HLA-C sequencing for each T cell clone and the control sample and DNA was isolated using a QIAprep Spin Miniprep Kit (QIAGEN, 27104). Sanger sequencing was performed using the M13F and M13R primers (Genewiz) ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from a tumor-reactive T cell culture using a Qiagen RNeasy Micro kit (Qiagen GmbH, Hilden, Germany). 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: Total genomic DNA from the sorted CD8+ T cells in the indicated groups were extracted using QIAamp DNA Micro Kit (Qiagen, 56304), eluted in a final volume of 50µL buffer AE and submitted for amplification and sequencing of the TCRβ region ...
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bioRxiv - Developmental Biology 2021Quote: RNA was extracted from appropriately staged embryos or whole larvae (n=10 /sample) using the RNeasy Plus Universal mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from heparinised blood of SI and SH mice (n=3 per housing group) using the RNeasy Protect Animal Blood Kit (Qiagen). Extracted RNA was hybridised at UCL genomics following standard Affymetrix protocols ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from blood (n=6 for each housing group) using the RNeasy Protect Animal Blood Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... 12 WT littermates) and two-month-old mice (adult, n = 4 per group) were lysed and homogenized in QIAzol Lysis Reagent (Qiagen). Total RNA purification was performed with the miRNeasy Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: Whole blood samples from 10 healthy volunteers (n = 5 females and n = 5 males) aged from 25 to 37 were collected into PAX gene RNA blood tubes (Qiagen). Total RNA samples were isolated using QIAcube automation with the PAXgene Blood miRNA Kit (Qiagen ...