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Citations for Qiagen :
601 - 650 of 1142 citations for Kallikrein 3 PSA Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... while rodent and human samples were eluted in 100 µl of buffer AE (Qiagen, Hilden, Germany). Samples were stored at −20°C before PCR.
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from murine and human monocytes with the RNeasy Plus Micro Kit (Qiagen), and then 200 ng of RNA was reverse transcribed using the iScript RT Supermix (Bio-Rad) ...
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bioRxiv - Genomics 2022Quote: ... cfDNA was then extracted from human blood plasma using the QIAmp Circulating Nucleic Acids kit (Qiagen), eluted in 60-μl elution buffer (10 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from mouse or human whole blood using RNA blood mini kit (Qiagen). Isolated RNA was converted to complementary DNA (cDNA ...
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bioRxiv - Immunology 2020Quote: ... cDNA was added to the Human Toll-like receptor signaling pathway RT2 Profiler PCR array (Qiagen) and run on an iCycler MyiQ (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Targets were included if biochemically confirmed using human tissue or non-species methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen, Germantown, MD). 0.5 μg Donor B RNA was mixed with 4.5 μg Donor A RNA to create sample C for quantitation experiments ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from cultured human and mouse cells using an RNeasy Mini Kit (Qiagen) and included an on-column DNase treatment to eliminate contaminating genomic DNA ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 5×106 human PBMCs from Cohort II using AllPrep RNEasy kits (Qiagen) and the small RNA-containing column flow-through collected ...
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bioRxiv - Microbiology 2021Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Cancer Biology 2020Quote: ... human FLMs and adjacent normal tissue was performed using the AllPrep DNA/RNA Mini Kit (Qiagen). Whole exome sequencing was performed by Novogene using their standard protocols ...
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bioRxiv - Pathology 2022Quote: ... Total RNA of mouse and human intestinal tissue was isolated using the RNeasy Mini Kit (Qiagen) and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... and human induced astrocytes (>day 30 of differentiation) were harvested in RLTplus Lysis buffer (Qiagen, 1053393). Total RNA was isolated using the RNeasy micro/mini plus kit (Qiagen ...
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bioRxiv - Physiology 2023Quote: ... and grown to confluency and transfected with human Leptin constructs using Effectene kit (Cat# 301427, QIAGEN).
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bioRxiv - Cancer Biology 2023Quote: gDNA was isolated from human sarcoma models and controls using the QIAamp DNA Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
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bioRxiv - Genetics 2024Quote: ... and aligned to a human reference sequence GRCh38/hg38 by using CLC Genomics Workbench v20 (Qiagen). The TPM (transcript per million ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from human primary immune cells using the RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from human sarcoma models and controls using the RNeasy Mini Kit (Qiagen). Library preparation was performed by the Functional Genomics Laboratory (FGL) ...
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bioRxiv - Developmental Biology 2021Quote: ... het and KO mice (3 each) by RNeasy Micro Kit with DNase I (Qiagen). Quality control was done by Qubit RNA assay ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were harvested after 3 or 7 days post-transfection (RNEasy mini kit, Qiagen), whereby cDNA was made and amplified via qPCR with primers for GAPDH ...
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bioRxiv - Systems Biology 2020Quote: ... 3 mL of culture were mixed with 6 mL of RNAprotect bacteria reagent (Qiagen) and processed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Genetics 2021Quote: ... 3×105 U2OS cells were reverse transfected with 5 pmol of each siRNA (QIAGEN) using lipofectamine RNAiMAX (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: ... cells or tissues were lysed using a TissueLyser II (3 min, 30 Hz; Qiagen) in RIPA buffer (150 mM NaCl (Merck) ...
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bioRxiv - Plant Biology 2021Quote: Plant tissue was disrupted mechanically using 3 mm tungsten carbide beads (Qiagen, Hilden, Germany), and a TissueLyser II (Quiagen ...
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bioRxiv - Immunology 2019Quote: ... 3 times and then eluted with 350 uL of buffer RLT (Qiagen RNeasy kit). Input and immunoprecipitated RNA was purified on column (Qiagen RNeasy kit ...
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bioRxiv - Plant Biology 2022Quote: ... from Brachypodium Bd21-3 genomic DNA extracted using the DNeasy Plant Mini Kit (Qiagen) or specific plasmids using primers that contained Type IIS restriction sites and overhangs complementary to the respective entry module ...
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bioRxiv - Microbiology 2023Quote: ... contaminated DNA was digested by 3 times treatment with RNase-Free DNase Set (Qiagen), and the absence of DNA contamination in extracted RNA was confirmed by PCR.
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bioRxiv - Molecular Biology 2022Quote: RNA was extracted from six hundred testes of ≤3 days old using miRNeasy (QIAGEN). Less than 200 nt fragments were collected using miRelute column (QIAGEN) ...
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bioRxiv - Genetics 2023Quote: ... and a single 3 mm metal bead (Eldan, BL6693003000) using TissueLyzer LT (QIAGEN, #85600) with a dedicated adaptor (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... for 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Vero cells were seeded in 24-well plates and incubated for 24 hours to reach confluency ...
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bioRxiv - Cell Biology 2023Quote: ... pools of 3 x 103 MPP1-4 were sorted directly into RLT buffer (Qiagen) enriched with 1% of β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were sorted into 384 well plates each containing 3 μl Vapor-Lock (Qiagen) in all wells and eight different conditions of 0.3 μl lysis buffer consisting of 0.125μM OligodT30VN (5’-Biotin-ACGAGCATCAGCAGCATACGAT30VN-3’ ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... frozen fragments of human cortex were homogenized with a motorized hand pestle in RLT lysis buffer (Qiagen) and qPCR was performed as described in qPCR section.
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bioRxiv - Microbiology 2021Quote: Genomic DNA (gDNA) from human blood cells was isolated using the QIAamp Blood kit (QIAGEN, Hilden, Germany). To isolate gDNA from different mouse tissues we made use of the blackPREP Rodent Tail DNA Kit (Analytik Jena ...
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bioRxiv - Cancer Biology 2020Quote: Total genomic DNAs (containing human and viral genomes) were isolated using AllPrep DNA/RNA Mini Kit (Qiagen) from the NPC biopsy ...
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bioRxiv - Cancer Biology 2020Quote: DNA from human and murine PC cells were isolated using the DNeasy blood and tissue kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Genomic human DNA was isolated from whole blood using QIAamp DNA Blood Mini Kit from Qiagen (51106) and phosphodiester DNA from TIB Molbiol ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Genetics 2019Quote: RNA was extracted from PCYT1A-wild-type human fibroblasts using the RNeasy Mini Kit (Qiagen, Cat#74104), reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Human ATGL-targeting and cPLA2α-targeting siRNAs and the AllStars Negative Control siRNA were from Qiagen (Germany). T863 (DGAT1 inhibitor) ...
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bioRxiv - Developmental Biology 2022Quote: ... Reads from *.fastq files were mapped to the rat reference genome (Rattus norvegicus reference genome Rnor_6.0) or the human reference genome (Homo sapiens reference genome GRCh37) using CLC Genomics Workbench 20.0.4 (Qiagen). Only reads with <2 mismatches and minimum length and a similarity fraction of 0.8 were mapped to the reference genome ...
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bioRxiv - Cell Biology 2023Quote: Total RNA of human macrophages was isolated according to manufacturer’s instructions using the RNeasy extraction kit (Qiagen). mRNA was extracted using the Next Poly(A ...
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bioRxiv - Cell Biology 2023Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using the RNeasy extraction kit (Qiagen). Two µg of RNA each was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: Genomic DNA was purified from human peripheral blood mononuclear cells (PBMCs) using QIAamp kits (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.