Labshake search
Citations for Qiagen :
1 - 50 of 231 citations for Integrin Alpha M CD11b ITGAM Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2021Quote: ... CD11b+ cells were collected in RNA lysis buffer (Qiagen) and processed for real time quantitative PCR analysis.
-
bioRxiv - Neuroscience 2024Quote: ... the enriched CD11b-positive cells were lysed using RLT buffer plus (Qiagen) supplemented with 40 μM DTT ...
-
bioRxiv - Neuroscience 2020Quote: ... and isolated microglia/macrophage (CD11b+ cells) using RNeasy plus Mini kit (Qiagen, 74134). cDNA synthesis was performed using QuantiTect Reverse Transcription Kit (Qiagen ...
-
bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from CD11b-positive cells using the RNeasy Micro Kit (Qiagen). Libraries were prepared using Smart-Seq2 64 and modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Microbiology 2020Quote: ... 0.1 M Tris (pH 7.0) (QIAGEN), 30% PEG 3000 (QIAGEN ...
-
bioRxiv - Neuroscience 2022Quote: ... CD11b+ CD45+ cells from one animal (2 retinas) were sorted directly into RLT buffer (QIAGEN 79216) and stored at -80°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
-
bioRxiv - Neuroscience 2020Quote: ... antibody previously bound to magnetic beads (Dynabeads M-280, Life Technologies. After reverse cross-linking and DNA purification (Qiagen Spin Column), ChIP-sequencing libraries were prepared using the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (E6240L ...
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from CD11b+ cells and LK cells using RNeasy plus mini kit (Qiagen 74134) [source?] ...
-
BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... 2 μL of 0.1 M Dithiothreitol (Qiagen), 1 μL of 10 mM each dNTP ...
-
bioRxiv - Neuroscience 2021Quote: ... CD11b+ magnetic sorted microglia cells or embryonic neural progenitor cells were homogenized in QIAzol Lysis Reagent (#79306, QIAGEN) either immediately after isolation or after 10 days of in vitro culturing ...
-
bioRxiv - Immunology 2022Quote: 5000-25000 P3/P4 LP macrophages from bone marrow chimeras 8 weeks following reconstitution were FACS-sorted (gated on: CD103-, Ly6G-, CD11b+, CD11cint, CD64+, Ly6C-, CD45.1+ or CD45.2+) directly into RLT buffer (Qiagen) on a FACS Aria II (BD Life Sciences ...
-
bioRxiv - Immunology 2022Quote: Total RNA from ~ 5 × 105 neutrophils (CD45+Lin-CD11b+Ly6G+) was isolated with the RNeasy micro kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Immunology 2023Quote: ... cytometry-sorted 7-AAD- CD45+CD11b+F4/80+ cells were lysed in 350µL RLT lysis buffer (Qiagen, 79216). At 4 days post injury ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23°C for 10 minutes at 14,000 x g ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23ºC for 10 minutes at 14,000 x g ...
-
bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
-
bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
-
bioRxiv - Neuroscience 2022Quote: ... CD11b+) were sorted with the Beckman Coulter MoFlo XDP and were collected in 350 µl RNA lysis buffer (Qiagen, 1053393), and stored at −80°C until further use ...
-
bioRxiv - Cancer Biology 2023Quote: ... epithelial cells (DAPI-EGFP+CD45-) and fibroblasts (DAPI-EGFP-CD45-CD11b-CD31-CD3-) were sorted and lysed in RLT buffer (Qiagen). Total RNA was prepared using RNeasy (Qiagen ...
-
bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
-
bioRxiv - Neuroscience 2021Quote: ... Lysis buffer was immediately added to CD11b+ cells for isolation of total RNA with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany); alternatively ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cloned plasmid was amplified in DH5-Alpha cells and purified with QIAfilter™ Plasmid Maxi Kit (Qiagen, catalogue number 12243). Plasmid concentration was measured using Qubit (Thermofisher ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with LZRS-vector constructs using Effectene transfection reagent (Qiagen, 301425) as follows ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.1 M Tris/Bicine pH 8.5 with 9 µL of JCSG+ (Qiagen) condition #29 containing 0.8 M sodium phosphate monobasic monohydrate ...
-
bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
-
bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
-
bioRxiv - Plant Biology 2020Quote: ... and ground for one minute at 20 m/s in a TissueLyser II (QIAGEN). Then ...
-
bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription for cDNA synthesis was performed using M-MuLV Reverse Transcriptase (Enzymatics, Qiagen) and RNAse Inhibitor (Biotechrabbit) ...
-
bioRxiv - Immunology 2023Quote: Total RNA was extracted from M-MDSCs using the miRNeasy Mini Kit (Qiagen, Germany). RNA libraries were prepared using TruSeq stranded mRNA sample prep kit (Illumina ...
-
bioRxiv - Cancer Biology 2021Quote: ... 4 PPKS/M and 4 PPKS/Y) using the AllPrep DNA/RNA Mini Kit (Qiagen). Indexed libraries were generates using the KAPA mRNA HyperPrep kit and sequenced on a NextSeq500 sequencer (NextSeq 500/550 High Output Kit v2.5 ...
-
bioRxiv - Bioengineering 2021Quote: RNA was extracted from ∼1 M cells using the QIAGEN RNeasy Mini Kit (QIAGEN 74104). A total of 36 samples were prepared ...
-
bioRxiv - Molecular Biology 2021Quote: ... STAT3 (M-003544-02) or non-targeting AllStars negative control siRNA (1027280) were purchased from Qiagen or Dharmacon ...
-
bioRxiv - Molecular Biology 2019Quote: ... with 0.2 M of IPTG at room temperature and was purified with Ni-NTA resin (Qiagen) with 50 mM sodium phosphate (pH 8.0) ...
-
bioRxiv - Cancer Biology 2021Quote: ... sections were incubated in 0.08 M KOH for 5 min and washed by Buffer EB (Qiagen). Then ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
-
bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
-
bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
-
bioRxiv - Genomics 2021Quote: ... One section was added to a MACs M tube (Miltenyi Biotech, U.K.) containing 1ml Qiazol (Qiagen, U.K.) and dissociated on a GentleMACs (Miltenyi Biotech ...
-
bioRxiv - Microbiology 2023Quote: ... followed by rigorous shaking for 40s at 5.5 m/s in a Tissuelyser II (Qiagen, Hilden, Germany). Serial dilutions of these samples were then plated on agar-solidified culture media (Fig ...
-
bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
-
bioRxiv - Plant Biology 2023Quote: ... with 0.2% (m/v) arabinose and the recombinant protein was affinity purified using Ni-NTA agarose (Qiagen). For GST-CPK3 ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
-
bioRxiv - Genomics 2019Quote: ... The partially decalcified nodule was then immersed in 1 mL of a digestion buffer with final concentrations of 0.45 M EDTA and 0.25 mg/mL Proteinase K (Qiagen) and rotated at 37°C overnight ...
-
bioRxiv - Neuroscience 2020Quote: Plcg2 knockdown was achieved in M-MØP cells using an antisense LNA GapmeR produced against mouse Plcg2 (Qiagen). Cells were grown to 80% confluence (∼2.4 × 105 cells per well ...
-
bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
-
bioRxiv - Plant Biology 2020Quote: ... Magnetic beads (Dynabeads M-270 Carboxylic Acid) were activated and coated with ad hoc designed LNA probes (Qiagen), harboring an −NH2 group ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
-
bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...