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Citations for Qiagen :
201 - 232 of 232 citations for Influenza Virus Hemagglutinin HA H5N1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... whereupon DNA was subsequently purified from 200 μl of the lysate using the QIAsymphony DSP Virus/Pathogen extraction kit (Qiagen Cat# 937036) on the QIAsymphony automated platform ...
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bioRxiv - Molecular Biology 2023Quote: Total viral genomic DNA was extracted from 100 μL stored plasma samples with the QIAamp MinElute Virus Spin Kit (Qiagen, Hilden, Germany), according to manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: Virus RNA from in vitro or in vivo experiments was isolated using the QIAamp® Vira RNA Mini Kit (Qiagen, Germantown, MD) or the MagMAX-96 AI/ND viral RNA isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... pMK33/NLS-Cas13d-NLS-HA-T2A-GFP was transfected into S2R+-NPT005 cells (DGRC # 229) using Effectene (Qiagen) according to manufacturer’s protocol and selected in 200 ng/mL Hygromycin B (Calbiochem ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were co-transfected with 0.6 µg HA-ACKR3-FlAsH-CFP and 1.2 µg K44A dynamin per dish using the Effectene reagent (Qiagen) and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen, Redwood City, CA) and SARS-CoV-CDC RUO primers and probes (Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids were extracted at CNM using either QIAamp MinElute Virus Spin (DNA) or QIAamp Viral RNA Mini kits (Qiagen, Germantown, MD, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... or Renilla-HA-NOD2 (750 ng/well) and His-FLAG-CAD (250 ng/well) using Effectene Transfection Reagent (Qiagen). The following day ...
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bioRxiv - Microbiology 2022Quote: ... Headless HA stalk proteins were expressed in 293F cells and purified using nickel-nitrilotriacetic acid agarose (no. 1018244, Qiagen) in 5-ml polypropylene columns (no ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were equalized and His6-HA-SUMO1-conjugates were enriched on nickel-nitrilotriacetic acid (NiNTA) agarose beads (Qiagen, #L30210) as described in15 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the full-length HA was amplified using gene-specific primers and the OneStep RT-PCR kit (Qiagen, #210212). The resulting PCR product was sequenced by Sanger sequencing (Genewiz) ...
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bioRxiv - Cancer Biology 2024Quote: ... A DNase treatment of RNA has been added before RNA Cleanup using the RNase-Free DNase set from Qiagen following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Neuroscience 2020Quote: ... The transfection of Wls-shRNA and pLCN-Wnt3a-HA and controls was performed by using HiPerFect transfection reagent (#301704, Qiagen) according to the manual ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: For SYBR Green and Taqman PCR Serial dilutions of known standards were made using synthetic miR-122 (syn has-miR-122-5p, 219600, Qiagen). The dilutions were prepared in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: ... IPs has been re-suspended in 1x beads volume of TE (10mM Tris pH8, 1mM EDTA) +0.5ng/ul RNaseA (Qiagen 158922) and 0.05U/ul of RNaseT1 (Sigma R1003 ...
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bioRxiv - Developmental Biology 2020Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 [60] and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... we transfected S2 cells in each well of a 6-well plate with 1 µg each of HA/GFP-tagged constructs using Effectene (Qiagen). After incubation for 3 days ...
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bioRxiv - Biochemistry 2021Quote: pAC5.1 vectors with CLIP-CRY and TIM-SNAP-HA were delivered into s2 cells using Effectene reagent (Cat# 301425, Qiagen) or TransIT-Insect Transfection Reagent (Cat# MIR6100 ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells in 6-well plates were transfected with 2 μg pIRES2-eGFP or Trim-HA-NUP153 derivatives using Effectene (Qiagen). At 24 h post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... using a forward primer that has a unique index and the reverse primer has another unique index for dual indexing (QIAseq miRNA 96 Index Kit IL UDI, #331645, Qiagen). Successful library production ...
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bioRxiv - Immunology 2023Quote: Total RNA has been extracted from cell lysates by using the RNeasy Plus Mini Kit (cat. 74136, Qiagen, Hilden, Germany). The total cDNA was obtained by reverse transcription using random primers from Invitrogen (ref ...
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bioRxiv - Developmental Biology 2023Quote: ... at a density of 1-2×106 cells/ml and transfected with pRmHA3-GAL4 (Klueg et al., 2002) and HA-tagged p10UAST-Robo or untagged pUAST-Slit plasmids using Effectene transfection reagent (Qiagen). GAL4 expression was induced with 0.5 mM CuSO4 for 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on fibronectin-treated coverslips and transfected with the K14-HA-Fzd-tdTomato constructs using the Effectene transfection reagent (Qiagen). Cells were switched to high Calcium media (1.5mM Ca2+ ...
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bioRxiv - Developmental Biology 2023Quote: Immunoprecipitation against HA-tagged exosomes was performed using Abcam Immunoprecipitation protocol on pan-exosomes isolated using the exoEasy kit (Qiagen). Protein G beads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the extracted and DNase treated RNA has been ribodepleted with the QIAseq Fast Select rRNA Plant Kit (Qiagen Hilden, Germany). Double stranded cDNAs were synthesized with random hexamer and the Maxima H Minus Kit (Thermo scientific ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid was extracted from 200 μl of each sample using the QIAamp MiniElute™ Virus Spin Kit nucleic acid (for DNA and RNA) according to the manufacturer (QIAgen Canada, Toronto, ON, Canada). DNA samples were stored at −80°C and assayed in duplicate by qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... BON1/TGL/pQ and BON1/TGL/HA-Bcl-xL) grown on 6-cm dishes were used to isolate mRNA using RNeasy Plus Mini kit (Qiagen, 74136) containing gDNA Eliminator spin columns ...
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bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
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bioRxiv - Cell Biology 2021Quote: ... The linearized vector DNA of pPB-CAG-mTpd52 ORF-HA was purified using a QIAquick Gel extraction kit (Qiagen, Chatsworth, CA, USA) and adjusted to a final concentration of 3 ng/μl in Tris-EDTA buffer for pronucleus injection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Quality of the final library has been evaluated through Qubit 4 and fragments sizes were checked by migration using QIAxcel Advanced Instrument (QIAgen, Hilden, Germany). Fragments ready for sequencing sized between 280bp and 320bp ...