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Citations for Qiagen :
51 - 100 of 247 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... were cotransfected with 0.35 μg of psPAX2 or pNL(AD8) and 0.45 μg of a reporter plasmid using Effectene transfection reagent (Qiagen, USA). The next day ...
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bioRxiv - Physiology 2019Quote: ... 1μM MG132 and protease inhibitors) using a TissueLyser II (Qiagen). After centrifugation at 13000 rpm for 15 min ...
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bioRxiv - Molecular Biology 2022Quote: ... was received from the External Quality Assurance Program Oversight Laboratory at Duke University,35 and extracted using QIAamp Viral RNA Mini Kits (Qiagen, DEU). The resulting viral RNA was quantified via quantitative real-time PCR ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from transgenic seedlings carrying 35S promoter-driven wild-type RAPTOR1B/ raptor1b or RAPTOR1BS916A/raptor1b and control seedlings using a Qiagen RNeasy kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Cells were lysed 72 hours post-transfection by directly adding 35 μl of β- mercaptoethanol-supplemented TCL buffer to wells and mRNA was extracted using TurboCapture 96 mRNA Kit (QIAGEN) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Immunology 2021Quote: ... Individuals were classified as Mtb-infected based on a positive QuantiFERON TB Gold In-tube assay (Qiagen; >0·35 IU/mL). All participants were healthy without signs or symptoms of active disease ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfections were carried out using just subconfluent cultures in 35 mm plates using DNA in the range of 0.3-2 µg/culture and the Polyfect reagent (Qiagen, Germantown, MD) and the manufacturer’s protocol (with 10 µl Polyfect reagent per 35 mm plate).
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bioRxiv - Cancer Biology 2022Quote: ... samples were homogenized using a tissue homogenizer (Brinkmann Instruments, Model PT 10/35, 110 Volts, 6 Amps, 60 Hz) for spleen and thymus while RLT (Qiagen, Hilden, Germany) and hand homogenization was used to isolate BM ...
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bioRxiv - Genetics 2022Quote: ... The pelleted worms were then purified from microbial contaminants using a 35% sucrose floatation.69 Genomic DNA was extracted from harvested worms using Genomic-tips (Qiagen Cat# 10223). The resulting high molecular weight genomic DNA was used to prepare sequencing libraries using the Illumina DNA Prep kit (Cat# 20018704 ...
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bioRxiv - Immunology 2024Quote: ... and colon (n = 35 each) and negative blank DNA extraction kit controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germantown, MD, USA), with minor modifications to the manufacturer’s protocol as previously described51–53 ...
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bioRxiv - Molecular Biology 2019Quote: ... the TEV protease was removed using Ni-NTA Agarose (QIAgen, 30210) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... and 1 μL of 1 AU/mL protease (Qiagen cat # 19157) to each well followed by a 60 min incubation at 55 °C ...
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bioRxiv - Genetics 2020Quote: ... supplemented with protease inhibitors and EDTA) volume using TissueLyzer equipment (Qiagen). Lysates were incubated on ice 20 minutes followed by centrifugation at 20,000xg for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed using Ni-NTA agarose (Qiagen). For FRET experiments ...
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bioRxiv - Cell Biology 2021Quote: ... SUMO-tag and protease were removed using Ni-NTA beads (Qiagen) and the protein of interest dialyzed in a sucrose buffer (250 mM sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Tween-20 in DEPC-treated TDW with protease (#19155, QIAGEN, 0.053 AU/ml] ...
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bioRxiv - Systems Biology 2023Quote: The tissues were cut into 30-35 mg pieces and homogenized using an automated homogenizer (Precelly®24; Bertin Technologies, France) with QIAzol® lysis reagent (Qiagen, USA). The RNA extraction was then performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... Proteases were precipitated using 200 ul of Protein Precipitation Solution (Qiagen, 158910) and centrifuged for 10 min at 12 000 g at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The recovered cells were unfixed by incubating with protease K (Qiagen, 19133) overnight at 56°C with shaking ...
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bioRxiv - Genomics 2019Quote: ... The embryos were treated with protease (1 µL of 25 µg/µL Qiagen Protease ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested with protease K in a concentration of 20 mg/ml (Qiagen). After several washing steps ...
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bioRxiv - Cell Biology 2021Quote: ... The His-tagged TEV protease was removed by incubation with Ni-NTA (Qiagen) overnight at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... which included the use of Qiagen Protease and carrier RNA (Qiagen Sciences, Maryland, MD). Samples were eluted in 75 μL of Buffer AVE (Qiagen Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... 15 μL of lysis buffer (Triton X-100 0.4%, Qiagen Protease 0.1 AU/mL) were added to each well and samples were incubated at 56 °C for 10 minutes and 72 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lysates were treated with 4 μl of Qiagen protease (QIAGEN, Hilden, Germany, #1017782,) at 56°C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Molecular Biology 2020Quote: ... and complete mini EDTA free protease inhibitor followed by mechanical homogenation by Tissue Ruptor (Qiagen). The samples were centrifuged at 13000 rpm for 15 mins and protein concentration calculated by BCA ...
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bioRxiv - Immunology 2022Quote: ... HRV3C protease was removed by incubating the elute with Ni-NTA resin (Qiagen, Düsseldorf, Germany). About 50 µl of elute containing released Birbeck granules (approximately 0.05 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1 % SDS) containing protease inhibitors and 0.1mM of 1,10- phenanthroline using a TissueLyser II (QIAGEN). The samples were then quantified ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with primary antibody (Penta·His Antibody, QIAGEN®, 1:2000 dilution) in 1% bovine serum albumin in PBST for 2h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and Halt Protease inhibitor (Thermo-Scientific) (1:100) for 1min at 30Hz using the TissuelyserLT (Qiagen). Homogenates were transferred to chilled Eppendorf tubes (−20°C ...
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bioRxiv - Microbiology 2019Quote: ... protease and phosphatase inhibitors) or RLT buffer supplemented with “- mercaptoethanol (RNeasy mini kit, Qiagen, Hilden, Germany) for western blot analysis and total RNA extraction ...
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bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Biochemistry 2023Quote: ... His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen) on gravity flow disposable plastic columns ...
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bioRxiv - Cell Biology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... After incubation with the primary antibody from QIAGEN (Penta-His Antibody, 1:1000 dilution) at RT for 1 h under shaking ...
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bioRxiv - Genetics 2022Quote: ... using biotinylated His5-antibody (Qiagen), as previously described (Asbury et al. ...
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bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5% deoxycholic acid with a protease inhibitor) and pre-cooled tissue homogenizer (TissueLyser LT, Qiagen GmbH, Hilden, Germany) for 2-4 min at 45 Hz ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... pH = 8) and genomic DNA was isolated as described previously53 with the following modifications: QIAGEN Protease ((#19157, QIAGEN) and PureLink RNAse A ((#12091021 ...
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bioRxiv - Cancer Biology 2020Quote: ... The samples were RNase-A and protease-K treated and finally eluted using the Qiagen PCR purification kit (Qiagen). The following antibodies were used (4 μg/sample) ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was homogenized in 150uL of RIPA buffer with protease and phosphatase inhibitors by bead homogenizer (Tissue LyserII, Qiagen) for 3 mins at a frequency of 30 hz/s and immediately cooled for 5 mins on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...