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Citations for Qiagen :
1 - 50 of 349 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: RNA was isolated from hBSMC microtissues that were either untreated or treated with TGF-β1 and IL-13 for 7 days prior to RNA isolation using the RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Cancer Biology 2020Quote: ... (13) and the manufacturer’s recommendations (Qiagen, 339450). In brief ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells and MEFs were transiently transfected with PolyFect (Qiagen) and Lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfection of HEK293 cells was performed using Attractene transfection reagent (Qiagen) by the fast-forward transfection approach following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or EGFP-Lin52 fusions into HEK293-GP cells with Effectene (Qiagen) for transduction as described previously (58).
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bioRxiv - Cell Biology 2021Quote: ... HEK293 or U20S cells with the RNeasy Mini-kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop 8000 spectrophotometer (Thermo-Fisher) ...
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bioRxiv - Biochemistry 2021Quote: Messenger RNA was extracted from HEK293 cells using an RNeasy Mini kit (Qiagen) according to the manufacturer’s instructions and used as the template to synthesise complementary DNA (cDNA ...
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bioRxiv - Biophysics 2024Quote: The HEK293 stable cell lines were generate by a transfection with Effectene (Qiagen) and 1µg plasmid (pmCherry-N1-hERG-WT and -A561V ...
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bioRxiv - Immunology 2020Quote: Total RNA of IFN-α2 treated HEK293 cells was purified using RNeasy columns (Qiagen) with on-column DNase I digestion ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were transfected with hTRPM3α2-GFP or its mutants using the Effectene reagent (Qiagen). Cells were loaded with 1 μM fura-2 AM (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339 ...
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bioRxiv - Immunology 2019Quote: ... IL-23p19 primers were purchased from QIAGEN and the sequence for osteopontin primers are ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757 ...
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bioRxiv - Cell Biology 2019Quote: ... by retrotranscription of RNAs from HeLa and HEK293-T cells using the QuantiTect Reverse Transcription kit (Qiagen) followed by PCR-mediated amplification and plasmid insertion with the in-Fusion cloning kit (Clontech) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was extracted from the parental and knockout HEK293 cells using QIAamp DNA mini kit (QIAGEN) and PCR amplified with primers located ~500-600 bp from the sgRNA target site ...
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bioRxiv - Genetics 2023Quote: The extraction of total RNA from HEK293 stable cell lines was isolated by RNeasy mini kit (QIAGEN), and cDNA was reversed with Maxima First Strand cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthetized from 13 ng total RNA with QuantiTect Reverse Transciption Kit (Qiagen 205313). Quantitative PCR reactions were carried out in triplicate with SYBR Green I Master Mix (Roche S-7563 ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from HEK293 cells using the QIAshredder and RNeasy Mini kit (Qiagen, 79654 and 74104, respectively). Briefly ...
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bioRxiv - Cell Biology 2024Quote: HEK293 cells were seeded in 15 cm plates and transfected after 24h according to manufacturer protocols (Effectene, Qiagen) using 5 µg of plasmid DNA ...
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bioRxiv - Microbiology 2019Quote: For qPCR measurements DNA was extracted from the 13 transformants using DNeasy Plant Mini Kit (Qiagen). qPCR primers were designed manually with the aid of SnapGene Viewer software ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA from mouse tissues and cultured HEK293 cells were extracted using DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD). Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research ...
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bioRxiv - Genetics 2022Quote: ... human SETD2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CRY2 siRNA (Qiagen # 1027416 ...
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bioRxiv - Genetics 2022Quote: ... human CPSF6 siRNA (Qiagen # 1027416 ...
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Coloring inside the lines: genomic architecture and evolution of a widespread color pattern in frogsbioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted from the skin of 13 individuals using a RNeasy mini kit (Qiagen, Valencia, CA). For eight individuals ...
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bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
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bioRxiv - Genomics 2022Quote: DNA was extracted from 13 mg of muscle tissue using a DNeasy Blood and Tissue kit (Qiagen), quantified using Qubit dsDNA HS Assay kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... organs from adult mice (9-13 weeks old) were harvested and homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by phenol–chloroform extraction according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from 13 ml of the culture using a DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer’s instructions for Gram-negative bacteria ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Physiology 2021Quote: RNA was isolated from the three HEK293 cell lines after 48 h in culture using the RNeasy Protect Mini Kit (catalog #74124, Qiagen). After reverse transcription (Super-Script II reverse transcriptase ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293 cells were transfected with plasmids (WT mGlu1, the mGlu1 mutants, or the control vector) using SuperFect transfection reagent (Qiagen) or Viafect (promega ...
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bioRxiv - Cell Biology 2021Quote: Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 5×106 HEK293 cells were used to isolate RNA with the All Prep RNA/DNA Mini Kit (Qiagen; 80204). cDNA was generated using 1μg of RNA with oligo(dT ...
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bioRxiv - Cell Biology 2024Quote: ... into 4 million HEK293-GP cells in 300 µl Buffer EC with 16 µl Enhancer and 60 µl Effectene Transfection Reagent (Qiagen 301425) (Morgenstern and Land ...
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bioRxiv - Cell Biology 2020Quote: Human DUOX1: GPH1004464(-)02A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: Human DUOX2: GPH1018346(-)08A (Qiagen)
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bioRxiv - Cell Biology 2020Quote: ... Human RPLP0 (primers from QIAGEN) was used as reference gene for cDNA from Caco-2 samples ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715 ...
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bioRxiv - Cancer Biology 2023Quote: ... with Quantitect human primers (Qiagen) for genes ID1 ...
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bioRxiv - Genomics 2019Quote: Bisulfite-pyrosequencing was performed to validate DNA methylation data from the Illumina 450K array for two CpG sites using specific primers (Supplementary Table 13) designed using PyroMark Assay Design software 2.0 (Qiagen). DNA methylation was quantified from intensities captured in the PyroMark Q24 pyrosequencer (Qiagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The samples were centrifuged and the supernatant mixed with 13 mL of a modified PB buffer (12.6 mL PB buffer (Qiagen), 6.5 μL Tween-20 ...
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bioRxiv - Microbiology 2019Quote: Vesicular RNA was obtained as previously described by our group (13, 34) with the mirCURYTM RNA isolation kit (Qiagen), used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Total genomic DNA was extracted from 13 faecal samples using the Qiagen QIAamp Mini Stool Kit (Qiagen, Hilden, Germany) as per the manufacture’s protocol with some modifications ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Binding buffer 13 times larger in volume was added to the concentrated supernatant and DNA was purified with MinElute columns (Qiagen) following the manufacturer’s instructions with the exception of a 15-minute incubation at 37 °C during the elution step ...
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bioRxiv - Immunology 2021Quote: ... Twenty consecutive 10 μm sections were placed on glass slides for subsequent IHC (sections 1-2, 7-8, 13-14, & 19-20) or placed in 600 μl buffer RLT (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... and larvae (12 CRISPR-treated, 20 water-injected and 13 uninjected) selected for sequencing by using DNeasy Blood & Tissue Kit (QIAGEN) Purity and concentration of DNA samples were measured with a NanoDrop spectrophotometer (Thermo Fisher Scientific).