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Citations for Qiagen :
1 - 50 of 1040 citations for IL 10 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... and CHO-K1 cells respectively using a RNA isolation kit (RNeasy Mini Kit) from Qiagen. cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits ...
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bioRxiv - Immunology 2023Quote: ... colonic Foxp3(GFP)+IL-10(Thy1.1)+ and Foxp3(GFP)+IL-10(Thy1.1)- cells were sorted into Trizol and total RNA was isolated with miRNeasy Micro Kits (QIAGEN 217084) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... RNA samples from heart tissue and HEK and CHO cell pellets were purified using a RNeasy mini kit (Qiagen). RNA was reverse-transcribed to cDNA using an Applied Biosystems High-Capacity RNA-to-cDNA kit (Foster City ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Immunology 2019Quote: ... IL-23p19 primers were purchased from QIAGEN and the sequence for osteopontin primers are ...
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bioRxiv - Neuroscience 2022Quote: ... the mouse spinal cord sections were treated with 10 μg/mL proteinase K (QIAGEN, 19131) at 37°C for 5 min and then post-fixed in 4% PFA at RT for 10 min ...
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bioRxiv - Systems Biology 2020Quote: ... IL-8 ELISA was performed according to manufacturer instructions (Qiagen).
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10 µL of mouse sera using QIAamp® viral RNA Mini Kit (Qiagen) or about 20 mg of organs homogenised by Precellys Lysing Kit Hard tissue grinding (Bertin Technologies ...
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bioRxiv - Microbiology 2019Quote: DNA for 16S rRNA gene sequencing was extracted from 10-50 mg fecal pellet from each mouse using the DNeasy Powersoil HTP 96 Kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2020Quote: Total mRNA was extracted from dissected IL using the RNeasy Micro kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The tube indices from the QIAseq miRNA NGS 48 Index IL (Qiagen, 331595) were used for library preparation.
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bioRxiv - Molecular Biology 2024Quote: ... IL]) and homogenized using a stainless steel bead in TissueLyser II (Qiagen, Germany) for 2 min at 30 Hz ...
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bioRxiv - Biochemistry 2022Quote: ... IL −6 and TNFα mRNA were detected by validated QuantiTect primer assays 144 (Qiagen).
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The gene primer for il-1α was a QuantiTect Primer Assay (Cat. No. QT00113505; Qiagen). The sequences for the remaining gene primers can be found in Table 1 and were ordered through Integrated DNA Technologies and diluted to 0.13 μM to be used for PCR ...
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bioRxiv - Cell Biology 2022Quote: ... IL-22ra1fl/fl/Shh-Cre or WT controls using RNAeasy mini kit (Qiagen, cat: 74106). Isolated RNA was converted into cDNA using a iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Gene specific primers for IL-6 (Hs_IL6_1_SG QuantiTect Primer Assay) was sourced from QuantiTect (Qiagen) primers ...
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bioRxiv - Microbiology 2021Quote: ... IL-6 and β-actin cDNA were determined by QuantiFast SYBR Green PCR Master Mix (Qiagen, Hilden, Germany) at the conditions ...
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bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted from dissected male and female IL or FAC sorted cells using the RNeasy Micro kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent HFK cell populations +/- IL-1β treatment using the RNeasy mini kit (Qiagen). PolyA selection ...
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bioRxiv - Genetics 2019Quote: ... The 10 μl reaction mixes contained1 μl of 10× reaction buffer (Qiagen), 0.8 μl dNTP ...
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bioRxiv - Biochemistry 2023Quote: The coding sequence of mouse Donson was amplified from XpressRef Universal Total mouse RNA (QIAGEN, 338114) by RT-PCR (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and siCASK #10 (Qiagen cat# SI04437720 ...
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bioRxiv - Immunology 2019Quote: ... StrEP-Tag (mouse monoclonal, Qiagen, #34850). Peroxidase-conjugated secondary antibodies against rabbit IgG (#7074 ...
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bioRxiv - Microbiology 2020Quote: ... mouse β-actin (PPM02945B-200, Qiagen), or MHV-A59 N gene using RT2 SYBR Green qPCR Mastermix (330502 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-His (Qiagen, number 34660); Mouse anti-β-actin (Proteintech ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-Strep (Qiagen, Cat# 34850), and mouse anti-Histidine (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: The siRNA against mouse VCP (Qiagen, catalog # ...
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bioRxiv - Biochemistry 2022Quote: ... A mouse monoclonal His-tetra (Qiagen) antibody (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... using a forward primer that has a unique index and the reverse primer has another unique index for dual indexing (QIAseq miRNA 96 Index Kit IL UDI, #331645, Qiagen). Successful library production ...
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bioRxiv - Immunology 2022Quote: ... Mouse and viral DNA were isolated from mouse tissue using the Qiagen DNeasy Blood and Tissue Kit (Qiagen). iTAQ universal Syber Green supermix (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-His tag (1:2000; QIAGEN) and rabbit anti-GST (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-His from Qiagen (1:10,000), home-made rabbit anti-VASH1 ...
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bioRxiv - Physiology 2019Quote: ... as well as mouse Tbp (Quantitect, Qiagen) as a housekeeping gene.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... primary mouse anti-His antibody (QIAGEN, 34660), secondary goat anti-rabbit 800CW antibody (LI-COR ...
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bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Mouse anti-His (Qiagen, 34670; 1:200) and goat anti-mouse IgG-AF488 (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Mouse XpressRef Universal Total RNA (QIAgen, 338114) was reverse transcribed and used at a concentration of 250 ng cDNA per reaction as a positive control for each primer set ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... Clones were verified by sequencing (ACGT; Wheeling, IL) and grown for midi-scale production and purification using a Qiagen Plasmid Plus Midiprep kit (Qiagen 12943).
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bioRxiv - Bioengineering 2022Quote: RNA was isolated from hBSMC microtissues that were either untreated or treated with TGF-β1 and IL-13 for 7 days prior to RNA isolation using the RNeasy Mini Kit (Qiagen 74106). The RNA yield was measured using Agilent TapeStation (4200 TapeStation System ...
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bioRxiv - Genomics 2020Quote: ... 10 µl Proteinase K (Qiagen), and 70 µl Digestion buffer (20mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl RDD buffer (QIAGEN) and 4 μl DNase I (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... mRNA was prepared from 10 dissected brains or 10 testes using the RNeasy kit (Qiagen). cDNA was then generated using the QuantiTect Reverse Transcription kit (Qiagen).
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Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... 10 μg/ml aprotinin and 10 μg/ml leupeptin) and passed through QIAshredder columns (Qiagen). Obtained protein samples were normalised ...
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bioRxiv - Cancer Biology 2022Quote: MIEV-or PKCα-KR-transduced HSPCs were co-cultured on OP9 cells in the presence of IL-7 for 17-23 days (late co-culture) and total RNA was isolated using an RNeasy kit (Qiagen, Manchester, UK) from five independent co-cultures ...