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Citations for Qiagen :
251 - 300 of 905 citations for IL 10 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... combined with 1:10 (v/v) proteinase K (Qiagen) treatment ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of 10 mg/mL RNAse A (Qiagen, 19101 ...
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bioRxiv - Molecular Biology 2024Quote: 20 μL EB (10 mM TrisCl, pH 8.5, Qiagen) were added to the reaction ...
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bioRxiv - Cell Biology 2024Quote: ... and around 10 colonies were mini-prepped (Qiagen #27107) and sequenced using Sanger DNA sequencing (Source Genomics ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Neuroscience 2020Quote: ... HSB was removed and beads were re-suspended in 400 µL supplemented RLT buffer (10 µL β-mercaptoethanol/10 mL RLT Buffer) from the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany) and vortexed vigorously ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Cancer Biology 2019Quote: ... and human BON1 cell line [8] were isolated with RNeasy Plus Universal Kits (Qiagen Cat no. 73404, Germantown, MD) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and was run on miScript miRNA PCR Array Human Ovarian Cancer plates (Qiagen, MIHS-110ZE-4, 384 well plate). PCR plates were read the ABI PRISM 7900HT Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... miScript miRNA PCR array of Human Neurological Development and Disease was performed using miScript SYBR green PCR kit (Qiagen). The PCR array contains 84 miRNAs which are differentially expressed during neuronal development and are responsible for progression of neurological diseases.
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: The human VCP cDNA was reverse transcribed from total RNA by using Omniscript RT kit (Qiagen Japan, Tokyo, Japan) extracted from A549 cells (RIKEN Cell Bank ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Immunology 2021Quote: ... followed by amplification and quantification using RT2 Profiler Human Innate and Adaptive Immune Response 96-well Array (Qiagen, 330231) with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequenced reads were mapped with the hg38 human genome and sequence analysis was done by the CLC genomics workbench (12.0.3, Qiagen Bioinformatics). Additionally ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from human tissue and cyst fluid using the QIAamp Mini kit (Qiagen, Hilden, Germany). Extractions were performed per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Raw FASTQ reads were aligned to the GRCh38 human genome using CLC Genomics Workbench version 22.0.2 software (Qiagen, USA). Differential gene expression was determined using the built-in tool in CLC Genomics workbench ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from human tissue derived organoids and neutrophils using the RNeasy® Plus Mini Kit (Qiagen, #74104) with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... in the presence of RNase A (10 μg/ml, Qiagen). In order to analyse DNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... A 10 nM solution of biotinylated penta-His antibody (Qiagen) was incubated for 10 min on the neutravidin-coated surface and excess unbound antibody removed (22 ...
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bioRxiv - Biophysics 2019Quote: ... 10 mL pre-equilibrated nickel agarose slurry (Ni-NTA; QIAGEN) was added per litre of medium and incubated for either 1 h at room temperature (RT ...
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bioRxiv - Developmental Biology 2019Quote: RNA was extracted from 10 midguts (females) using RNeasy (Qiagen). One microgram of RNA was reverse transcribed using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas) ...
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bioRxiv - Genetics 2021Quote: ... 10 mM Imadizole was incubated with Ni-NTA beads (Qiagen) and the bound protein of interest was eluted using increasing concentrations of Imidazole ...
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bioRxiv - Genomics 2021Quote: ... 1.3 μL of 10 mg/mL RNase A (Qiagen #1007885) was added and incubated at 37 °C for 30 min to remove RNA ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of protease mix (100 μg protease (Qiagen, 19155), 1x GC reaction buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... treated for 10-30 minutes with Taq polymerase (Qiagen, 201203) to generate Adenine overhangs ...
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bioRxiv - Genomics 2021Quote: ... and 10 μL of 20 mg/mL proteinase K (Qiagen) and incubated at 60 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Buffer EB (10 mM Tris-Cl, pH 8.5, Qiagen, Germany) was used ...
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bioRxiv - Microbiology 2021Quote: ... Tris 10 mM) supplemented with 1% Proteinase K (Qiagen #19133) and 1% RNAse A/T1 (ThermoFisher #EN0551 ...
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bioRxiv - Neuroscience 2022Quote: ... 10 fly heads per condition were homogenized in Qiazol (Qiagen) and total RNA was isolated ...
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bioRxiv - Microbiology 2022Quote: ... and 1 μL of 10 mg/mL RNase A (Qiagen), then incubating at 37°C for 1.5 hr without shaking ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was immediately treated with 10 mL RNAprotect (Qiagen) and incubated at room temperature for 5 min ...
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bioRxiv - Genomics 2023Quote: ... After adding 10 µL RNase A (100 mg/mL, Qiagen) and 250 µL Proteinase K (20 mg/mL ...
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bioRxiv - Physiology 2023Quote: ... The first method was with CLC Genomics (Qiagen, version 10) based on previous methods (Benoit et al ...
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bioRxiv - Molecular Biology 2024Quote: ... 2x Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5), 1x rCutsmart Buffer.
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bioRxiv - Cell Biology 2020Quote: ... The cDNA (200 ng) was used for quantitative PCR with the Human Cellular Senescence RT2 Profiler™ PCR Array (Qiagen) containing 84 key genes involved in the initiation and progression of the biological process causing cells to lose the ability to divide ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Molecular Biology 2019Quote: Preliminary screening for the presence of serum exosomal miRNAs was determined using a miScript human miFinder PCR array (MIHS-001Z, Qiagen). RNA was converted to cDNA using miScript RT kit (218060) ...
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bioRxiv - Plant Biology 2019Quote: ... three replicates of 25 to 30 pooled egg cells were used for total RNA extraction according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (Qiagen). In brief ...
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bioRxiv - Genetics 2020Quote: ... Analyses of HTT CAG repeat size in both HttQ111 mice and in patient fibroblasts was performed by PCR using human-specific HTT primers and Taq PCR Core Kit with Q solution (Qiagen), as previously described (17 ...
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...