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Citations for Qiagen :
651 - 700 of 10000+ citations for Human Transcription factor HIVEP2 HIVEP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Total RNAs were obtained from human UREC or mouse kidneys using RNeasy Mini Kit (Qiagen) and reverse transcribed using SuperScript II Reverse Transcriptase (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from human organoids using RNeasy Mini Kit with DNase treatment (QIAGEN), and synthesis of cDNA was conducted with High-Capacity cDNA Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: Total RNA was extracted from human or murine cells using the RNeasy Mini Kit (Qiagen), and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit ...
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bioRxiv - Immunology 2020Quote: ... total mRNA was isolated from mouse and human B cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Physiology 2020Quote: ... RNA from human islets (∼150 for each donor) was extracted with RNeasy Mini Kit (Qiagen) and was reverse transcribed using the High Capacity Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was extracted from human tissue and cells using the miRNeasy Mini kit (Qiagen) or TRIzol reagent (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... For the RT² Profiler™ PCR Array Human Epithelial to Mesenchymal Transition kit (EMT) (Qiagen), RNA integrity of all the samples was tested by using the Agilent RNA 6000 Nano kit (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from sorted infected primary human hepatocytes using miRNeasy Micro Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from human primary cells using AllPrep RNA/RNA/miRNA universal kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from pigskin and human skin swabs using a DNeasy PowerSoil kit (Qiagen). Procedural extraction control blanks (swabs with sterile water ...
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bioRxiv - Cell Biology 2023Quote: ... and human islets was isolated using RNeasy Mini or Micro kits (Qiagen, Valencia, CA, USA). Reverse transcription was performed using a High Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from sampled human brains using miRNeasy Mini Kit (Qiagen, CA, USA). The tissue samples were homogenized in QIAzol (Qiagen ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: RNA isolation and reverse transcription for murine samples from the GRN-FTD proof of concept study was completed using the QIAsymphony RNA kit (Qiagen). Absolute quantification of transgene copies per microgram of tissue was completed using a standard curve of in vitro transcribed WPRE RNA and specific primers and probes targeting WPRE (WPRE v2 ...
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bioRxiv - Physiology 2020Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Neuroscience 2019Quote: 1μg of RNA was used for cDNA synthesis prepared from the Quantitect Reverse Transcription Kit according to the manufactures’ protocol (Qiagen). Quantitative PCR was then performed on a RotorGeneQ (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was quantified using the Nano-Drop 8000 (Thermo Fischer) followed by cDNA synthesis using QuantiTect Reverse Transcription kit (Qiagen). The primers for PCR with reverse transcription reactions are listed in Supplemental Table 2 ...
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bioRxiv - Molecular Biology 2019Quote: Reverse transcription (RT) of total RNA to cDNA was performed in 50 μL reaction volumes using the miRCURY RT kit (Qiagen) and 10 μL total RNA as input ...
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bioRxiv - Microbiology 2019Quote: ... 500 ng of RNA were reverse transcribed in a final volume of 20 µl using QuantiTect Reverse Transcription Kit (Qiagen). Real-time PCR was performed using SYBR Green PowerUp (ThermoScientific ...
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bioRxiv - Microbiology 2021Quote: Five nanograms of mRNA were analyzed by quantitative reverse transcription-PCR (qRT-PCR) using the QuantiFast SYBR green RT-PCR kit (Qiagen). qRT-PCR-based quantification of IL-6 ...
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bioRxiv - Bioengineering 2021Quote: ... 600 ng of total RNA was used for complementary DNA (cDNA) synthesis using a QuantiTect Reverse Transcription Kit from Qiagen following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from isolated brains as described before42 and using the QuantiTect Reverse Transcription Kit (QIAGEN Cat. No. 205311) 200ng of RNA was transcribed into cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... specific to the DNA sequence (or cDNA sequence from reverse transcribed clone G2 RNA samples (QuantiTect Reverse Transcription Kit, Qiagen)) for PCR (PCR SuperMix High Fidelity ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 0.8 µg of RNA was used for cDNA synthesis according to manufacturer’s instructions of the QuantiTect® Reverse Transcription Kit (Qiagen). 1 µl of cDNA was used for semi-quantitative reverse-transcriptase polymerase chain reaction ...
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bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was retro-transcribed into complementary DNA (cDNA) by using the QuantiTect Reverse Transcription Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen) on an iCycler instrument (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... RNA sample was treated with DNase I to remove genomic DNA and retro transcribed into cDNA using Quantitect Reverse Transcription Kit (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 μg were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Microbiology 2019Quote: RNA was harvested from late-log phase Mtb and the 3’ end of Mcr11 was mapped using the miScript Reverse Transcription Kit (Qiagen) (88 ...
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bioRxiv - Immunology 2020Quote: ... mRNAs recovered from microdissected samples or vaginal biopsies (about 1 mm3) were reverse-transcribed with the QuantiTect Rev Transcription Kit (Qiagen), used according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... for various immune genes were used to amplify cDNA collected from lungs using QuantiNova reverse transcription and SYBR Green I PCR kits (Qiagen). The ΔΔCq method was used with normalization within sample on GAPDH (ΔCq ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed on three biological replicates of each in planta time point and two biological replicates for the mycelia timepoints with the QuantiTech Reverse Transcription Kit (Qiagen). In planta timepoints ...
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bioRxiv - Immunology 2021Quote: ... A total of 1-2 μg of RNA was used to synthesize the first single-strand cDNA using QuantiTect Reverse Transcription kit (Qiagen). For RT-PCR amplification ...
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bioRxiv - Immunology 2021Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesised from every 1 μg of total mRNA in 20 μL volume with QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacture protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5 µg RNA was converted into cDNA using the Quantitect® Reverse Transcription Kit (Cat. No. 205311, Qiagen, Limburg, Netherlands). To ensure the removal of genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA concentration and purity were measured by a spectrophotometer and 2 ug were used to synthesize cDNA by QuantiTect Reverse Transcription kit (Qiagen). Real-time amplification was performed in a Mastercycler EP Realplex (Eppendorf ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were run on 1 μg of RNA per sample to afford cDNA using the QuantiTect Reverse Transcription kit (Qiagen). RT-qPCR was performed on an Applied Biosciences 7500 Real-Time PCR system following the QuantiTect SYBR Green PCR kit protocols (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... ML336 resistant mutation emerging in nsP2 at passage 10 was confirmed by purification and reverse transcription of viral RNA from cell supernatants using RNeasy Mini Kit (Qiagen) and SuperScript IV First-Strand Synthesis kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized from total input RNA and oligo(dT)-isolated RNA with the QuantiTect Reverse Transcription Kit (205311, Qiagen). All qRT-PCRs were performed with the SYBR green method ...
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bioRxiv - Developmental Biology 2020Quote: ... (underlined region is the T7 promoter for In vitro transcription) These primers amplified exonic region of Dyrk1b and the product was cleaned using PCR purification kit (Qiagen)and cloned into pGEM®-T Easy Vector Systems (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from a pool of 20 mechanically homogenized embryos at 96 hpf for each sample (n=4) using TRItidy G (AppliChem) and 500 ng of cDNA was synthesized using QuantiTect Reverse Transcription Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Equal amounts of total RNA from each samples were then reverse-transcribed into cDNA using QuantiTect Reverse Transcription Kit (QIAGEN). qRT-PCR was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... genomic DNA was eliminated with a gDNA Wipeout Buffer and subsequently reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). The diluted cDNA was prepared with SYBR Green (Roche) ...
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bioRxiv - Plant Biology 2022Quote: ... DNase treatment to remove contamination of genomic DNA and first-strand cDNA synthesis were performed using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time RT-PCR (qPCR ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) in technical triplicate using the QuantiNova SYBR Green PCR kit (Qiagen). mRNA levels were normalized to the geometric mean of RPL11 ...
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bioRxiv - Physiology 2023Quote: ... were normalized at 10 ng/µL and the reverse transcription of RNA was done following the manufacturer instructions (miRCURY LNA RT Kit, Qiagen). We added 0.5µl of controls UniSp6 and cel-mir-39-3p to the samples as internal reference to check the efficiency of the reverse transcription and PCR amplification ...
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bioRxiv - Cancer Biology 2022Quote: ... reverse transcription of RNA extracted from OECM-1 cells transfected with miRNA mimics and SC control was carried out using the Quantitect Reverse Transcription Kit (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared from 2μg total RNA from each sample under the instruction of QuantiTech reverse transcription Kit (Qiagen, 205311). Samples for QPCR were then prepared under the instruction of ONEGreen fast qPCR kit (Ozyme ...
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bioRxiv - Cell Biology 2023Quote: ... gDNA wipeout buffer was used to remove Genomic DNA for 2 minutes at 42°C and cDNA was synthetized with the QuantiTect Reverse Transcription Kit (Qiagen). qPCR was performed with PowerUp SYBR Green Master Mix using the StepOnePlus Real-Time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: cDNA was produced from 1 μg of total RNA (final cDNA concentration 5 ng/ml) using QuantiTech Reverse Transcription kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...